Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12070 and BWI76_RS12075 are separated by 284 nucleotides BWI76_RS12075 and BWI76_RS12080 are separated by 140 nucleotides BWI76_RS12080 and BWI76_RS12085 are separated by 2 nucleotides BWI76_RS12085 and BWI76_RS12090 are separated by 11 nucleotides
BWI76_RS12070: BWI76_RS12070 - hypothetical protein, at 2,537,367 to 2,537,561
_RS12070
BWI76_RS12075: BWI76_RS12075 - regulator, at 2,537,846 to 2,538,406
_RS12075
BWI76_RS12080: BWI76_RS12080 - hypothetical protein, at 2,538,547 to 2,538,777
_RS12080
BWI76_RS12085: BWI76_RS12085 - regulatory protein CII from bacteriophage origin, at 2,538,780 to 2,539,316
_RS12085
BWI76_RS12090: BWI76_RS12090 - HNH endonuclease, at 2,539,328 to 2,539,831
_RS12090
Position (kb)
2538
2539 Strain fitness (log2 ratio)
-1
0
1
2 at 2537.559 kb on - strand at 2537.608 kb on + strand at 2537.697 kb on + strand at 2538.634 kb on - strand, within BWI76_RS12080 at 2538.687 kb on + strand, within BWI76_RS12080 at 2538.703 kb on - strand, within BWI76_RS12080 at 2538.778 kb on - strand at 2538.860 kb on + strand, within BWI76_RS12085 at 2538.880 kb on - strand, within BWI76_RS12085 at 2538.900 kb on - strand, within BWI76_RS12085 at 2538.983 kb on + strand, within BWI76_RS12085 at 2538.983 kb on + strand, within BWI76_RS12085 at 2538.991 kb on - strand, within BWI76_RS12085 at 2539.183 kb on + strand, within BWI76_RS12085 at 2539.188 kb on + strand, within BWI76_RS12085 at 2539.258 kb on + strand, within BWI76_RS12085 at 2539.273 kb on - strand at 2539.278 kb on + strand at 2539.302 kb on - strand at 2539.339 kb on - strand at 2539.339 kb on - strand at 2539.500 kb on - strand, within BWI76_RS12090 at 2539.558 kb on + strand, within BWI76_RS12090 at 2539.566 kb on - strand, within BWI76_RS12090 at 2539.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 2,537,559 - -0.2 2,537,608 + -0.9 2,537,697 + +0.1 2,538,634 - BWI76_RS12080 0.38 -0.3 2,538,687 + BWI76_RS12080 0.61 -1.1 2,538,703 - BWI76_RS12080 0.68 -0.5 2,538,778 - -0.2 2,538,860 + BWI76_RS12085 0.15 +0.4 2,538,880 - BWI76_RS12085 0.19 +0.1 2,538,900 - BWI76_RS12085 0.22 -0.1 2,538,983 + BWI76_RS12085 0.38 +0.7 2,538,983 + BWI76_RS12085 0.38 +0.1 2,538,991 - BWI76_RS12085 0.39 -0.9 2,539,183 + BWI76_RS12085 0.75 +0.4 2,539,188 + BWI76_RS12085 0.76 +1.1 2,539,258 + BWI76_RS12085 0.89 +1.5 2,539,273 - -0.2 2,539,278 + +0.3 2,539,302 - +0.5 2,539,339 - +0.3 2,539,339 - +2.0 2,539,500 - BWI76_RS12090 0.34 -0.1 2,539,558 + BWI76_RS12090 0.46 +0.5 2,539,566 - BWI76_RS12090 0.47 +0.0 2,539,723 + +0.4
Or see this region's nucleotide sequence