Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10860 and BWI76_RS10865 are separated by 288 nucleotides BWI76_RS10865 and BWI76_RS10870 are separated by 145 nucleotides BWI76_RS10870 and BWI76_RS10875 are separated by 20 nucleotides BWI76_RS10875 and BWI76_RS10880 are separated by 114 nucleotides
BWI76_RS10860: BWI76_RS10860 - ACR family transporter, at 2,294,529 to 2,297,603
_RS10860
BWI76_RS10865: BWI76_RS10865 - tRNA-Ser, at 2,297,892 to 2,297,979
_RS10865
BWI76_RS10870: BWI76_RS10870 - nicotinamidase, at 2,298,125 to 2,298,802
_RS10870
BWI76_RS10875: BWI76_RS10875 - quercetin 2,3-dioxygenase, at 2,298,823 to 2,299,683
_RS10875
BWI76_RS10880: BWI76_RS10880 - LysR family transcriptional regulator, at 2,299,798 to 2,300,703
_RS10880
Position (kb)
2298
2299 Strain fitness (log2 ratio)
-1
0
1 at 2297.198 kb on + strand, within BWI76_RS10860 at 2297.455 kb on + strand at 2297.629 kb on - strand at 2298.029 kb on - strand at 2298.309 kb on - strand, within BWI76_RS10870 at 2298.319 kb on - strand, within BWI76_RS10870 at 2298.713 kb on - strand, within BWI76_RS10870 at 2298.951 kb on + strand, within BWI76_RS10875 at 2299.164 kb on - strand, within BWI76_RS10875 at 2299.447 kb on + strand, within BWI76_RS10875
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 2,297,198 + BWI76_RS10860 0.87 -0.3 2,297,455 + +1.5 2,297,629 - +0.3 2,298,029 - -0.2 2,298,309 - BWI76_RS10870 0.27 -0.2 2,298,319 - BWI76_RS10870 0.29 -1.4 2,298,713 - BWI76_RS10870 0.87 -0.6 2,298,951 + BWI76_RS10875 0.15 -0.4 2,299,164 - BWI76_RS10875 0.40 -1.0 2,299,447 + BWI76_RS10875 0.72 +0.6
Or see this region's nucleotide sequence