Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00100 and BWI76_RS00105 are separated by 37 nucleotides BWI76_RS00105 and BWI76_RS00110 are separated by 48 nucleotides BWI76_RS00110 and BWI76_RS00115 are separated by 164 nucleotides BWI76_RS00115 and BWI76_RS00120 are separated by 41 nucleotides
BWI76_RS00100: BWI76_RS00100 - hypothetical protein, at 19,469 to 20,206
_RS00100
BWI76_RS00105: BWI76_RS00105 - NAD(P)H-dependent oxidoreductase, at 20,244 to 20,810
_RS00105
BWI76_RS00110: BWI76_RS00110 - adenine permease PurP, at 20,859 to 22,199
_RS00110
BWI76_RS00115: BWI76_RS00115 - 6-phosphogluconate phosphatase, at 22,364 to 23,029
_RS00115
BWI76_RS00120: BWI76_RS00120 - glucosamine-6-phosphate deaminase, at 23,071 to 23,808
_RS00120
Position (kb)
20
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1 at 19.967 kb on + strand, within BWI76_RS00100 at 20.030 kb on + strand, within BWI76_RS00100 at 20.038 kb on - strand, within BWI76_RS00100 at 20.053 kb on - strand, within BWI76_RS00100 at 20.123 kb on + strand, within BWI76_RS00100 at 20.244 kb on - strand at 20.281 kb on - strand at 20.314 kb on + strand, within BWI76_RS00105 at 20.322 kb on - strand, within BWI76_RS00105 at 20.350 kb on - strand, within BWI76_RS00105 at 20.436 kb on - strand, within BWI76_RS00105 at 20.467 kb on - strand, within BWI76_RS00105 at 20.495 kb on - strand, within BWI76_RS00105 at 20.502 kb on + strand, within BWI76_RS00105 at 20.588 kb on - strand, within BWI76_RS00105 at 20.715 kb on - strand, within BWI76_RS00105 at 20.806 kb on - strand at 20.835 kb on - strand at 20.856 kb on + strand at 20.856 kb on + strand at 20.864 kb on - strand at 20.929 kb on - strand at 20.929 kb on - strand at 20.989 kb on - strand at 21.080 kb on + strand, within BWI76_RS00110 at 21.359 kb on - strand, within BWI76_RS00110 at 21.459 kb on + strand, within BWI76_RS00110 at 21.538 kb on + strand, within BWI76_RS00110 at 21.578 kb on - strand, within BWI76_RS00110 at 21.737 kb on + strand, within BWI76_RS00110 at 21.957 kb on - strand, within BWI76_RS00110 at 22.121 kb on - strand at 22.125 kb on - strand at 22.130 kb on - strand at 22.416 kb on - strand at 22.506 kb on - strand, within BWI76_RS00115 at 22.559 kb on - strand, within BWI76_RS00115 at 22.675 kb on - strand at 22.686 kb on + strand, within BWI76_RS00115 at 22.694 kb on - strand, within BWI76_RS00115 at 22.854 kb on - strand, within BWI76_RS00115 at 22.890 kb on - strand, within BWI76_RS00115 at 23.039 kb on + strand at 23.076 kb on - strand at 23.081 kb on - strand at 23.133 kb on + strand at 23.170 kb on - strand, within BWI76_RS00120
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 19,967 + BWI76_RS00100 0.67 -0.3 20,030 + BWI76_RS00100 0.76 +0.7 20,038 - BWI76_RS00100 0.77 -0.1 20,053 - BWI76_RS00100 0.79 -0.6 20,123 + BWI76_RS00100 0.89 -1.2 20,244 - -0.3 20,281 - -0.5 20,314 + BWI76_RS00105 0.12 +0.3 20,322 - BWI76_RS00105 0.14 -0.5 20,350 - BWI76_RS00105 0.19 -0.9 20,436 - BWI76_RS00105 0.34 -0.3 20,467 - BWI76_RS00105 0.39 -0.4 20,495 - BWI76_RS00105 0.44 -0.6 20,502 + BWI76_RS00105 0.46 -0.2 20,588 - BWI76_RS00105 0.61 -0.0 20,715 - BWI76_RS00105 0.83 +0.2 20,806 - -0.1 20,835 - +0.5 20,856 + -0.4 20,856 + -0.8 20,864 - -0.3 20,929 - +0.2 20,929 - -0.3 20,989 - -0.9 21,080 + BWI76_RS00110 0.16 -0.6 21,359 - BWI76_RS00110 0.37 -0.0 21,459 + BWI76_RS00110 0.45 -1.1 21,538 + BWI76_RS00110 0.51 -1.0 21,578 - BWI76_RS00110 0.54 -0.3 21,737 + BWI76_RS00110 0.65 -1.9 21,957 - BWI76_RS00110 0.82 -0.1 22,121 - -0.1 22,125 - -1.0 22,130 - -0.1 22,416 - -0.1 22,506 - BWI76_RS00115 0.21 -0.3 22,559 - BWI76_RS00115 0.29 -0.7 22,675 - +1.1 22,686 + BWI76_RS00115 0.48 -0.4 22,694 - BWI76_RS00115 0.50 -0.1 22,854 - BWI76_RS00115 0.74 -0.3 22,890 - BWI76_RS00115 0.79 -0.9 23,039 + -0.3 23,076 - -0.5 23,081 - -1.4 23,133 + -0.6 23,170 - BWI76_RS00120 0.13 -1.0
Or see this region's nucleotide sequence