Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13030

Experiment: KLEB15 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13020 and BWI76_RS13025 are separated by 137 nucleotidesBWI76_RS13025 and BWI76_RS13030 are separated by 214 nucleotidesBWI76_RS13030 and BWI76_RS13035 are separated by 228 nucleotides BWI76_RS13020: BWI76_RS13020 - NmrA family protein, at 2,716,832 to 2,717,821 _RS13020 BWI76_RS13025: BWI76_RS13025 - histidine phosphatase family protein, at 2,717,959 to 2,718,579 _RS13025 BWI76_RS13030: BWI76_RS13030 - site-specific recombinase, phage integrase family protein, at 2,718,794 to 2,719,396 _RS13030 BWI76_RS13035: BWI76_RS13035 - MFS transporter, at 2,719,625 to 2,721,079 _RS13035 Position (kb) 2718 2719 2720Strain fitness (log2 ratio) -1 0 1at 2718.049 kb on + strand, within BWI76_RS13025at 2718.294 kb on + strand, within BWI76_RS13025at 2718.419 kb on + strand, within BWI76_RS13025at 2718.503 kb on + strand, within BWI76_RS13025at 2718.633 kb on + strandat 2718.776 kb on + strandat 2719.149 kb on + strand, within BWI76_RS13030at 2719.174 kb on + strand, within BWI76_RS13030at 2719.460 kb on + strandat 2719.604 kb on - strandat 2719.681 kb on - strandat 2719.692 kb on + strandat 2719.737 kb on + strandat 2719.737 kb on + strandat 2719.745 kb on - strandat 2719.745 kb on - strandat 2719.745 kb on - strandat 2719.747 kb on + strandat 2719.799 kb on + strand, within BWI76_RS13035at 2719.807 kb on - strand, within BWI76_RS13035at 2719.835 kb on - strand, within BWI76_RS13035at 2720.016 kb on - strand, within BWI76_RS13035at 2720.018 kb on + strand, within BWI76_RS13035at 2720.026 kb on - strand, within BWI76_RS13035at 2720.043 kb on + strand, within BWI76_RS13035at 2720.046 kb on - strand, within BWI76_RS13035at 2720.089 kb on + strand, within BWI76_RS13035at 2720.108 kb on - strand, within BWI76_RS13035at 2720.145 kb on - strand, within BWI76_RS13035at 2720.148 kb on + strand, within BWI76_RS13035at 2720.199 kb on + strand, within BWI76_RS13035at 2720.226 kb on - strand, within BWI76_RS13035at 2720.226 kb on - strand, within BWI76_RS13035at 2720.354 kb on + strand, within BWI76_RS13035at 2720.354 kb on + strand, within BWI76_RS13035at 2720.369 kb on + strand, within BWI76_RS13035at 2720.377 kb on - strandat 2720.377 kb on - strand, within BWI76_RS13035

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1
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2,718,049 + BWI76_RS13025 0.14 -1.1
2,718,294 + BWI76_RS13025 0.54 -0.1
2,718,419 + BWI76_RS13025 0.74 -1.3
2,718,503 + BWI76_RS13025 0.88 -1.3
2,718,633 + -0.0
2,718,776 + -0.6
2,719,149 + BWI76_RS13030 0.59 -1.2
2,719,174 + BWI76_RS13030 0.63 -0.5
2,719,460 + +1.1
2,719,604 - -1.2
2,719,681 - -0.5
2,719,692 + +0.7
2,719,737 + +1.7
2,719,737 + +1.0
2,719,745 - -0.2
2,719,745 - +0.1
2,719,745 - -0.1
2,719,747 + -0.6
2,719,799 + BWI76_RS13035 0.12 -0.9
2,719,807 - BWI76_RS13035 0.13 -0.0
2,719,835 - BWI76_RS13035 0.14 +0.2
2,720,016 - BWI76_RS13035 0.27 -0.5
2,720,018 + BWI76_RS13035 0.27 +0.1
2,720,026 - BWI76_RS13035 0.28 +0.2
2,720,043 + BWI76_RS13035 0.29 -0.4
2,720,046 - BWI76_RS13035 0.29 +0.9
2,720,089 + BWI76_RS13035 0.32 -0.3
2,720,108 - BWI76_RS13035 0.33 -0.5
2,720,145 - BWI76_RS13035 0.36 -0.3
2,720,148 + BWI76_RS13035 0.36 -0.1
2,720,199 + BWI76_RS13035 0.39 -0.4
2,720,226 - BWI76_RS13035 0.41 -0.2
2,720,226 - BWI76_RS13035 0.41 -0.7
2,720,354 + BWI76_RS13035 0.50 +0.1
2,720,354 + BWI76_RS13035 0.50 -0.3
2,720,369 + BWI76_RS13035 0.51 -0.8
2,720,377 - +1.4
2,720,377 - BWI76_RS13035 0.52 +0.3

Or see this region's nucleotide sequence