Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21380 and BWI76_RS21385 are separated by 55 nucleotides BWI76_RS21385 and BWI76_RS21390 are separated by 203 nucleotides BWI76_RS21390 and BWI76_RS21395 are separated by 69 nucleotides
BWI76_RS21380: BWI76_RS21380 - uracil-DNA glycosylase, at 4,475,640 to 4,476,329
_RS21380
BWI76_RS21385: BWI76_RS21385 - tRNA/rRNA methyltransferase, at 4,476,385 to 4,477,458
_RS21385
BWI76_RS21390: BWI76_RS21390 - thiol disulfide reductase thioredoxin, at 4,477,662 to 4,478,087
_RS21390
BWI76_RS21395: BWI76_RS21395 - DTW domain-containing protein, at 4,478,157 to 4,478,855
_RS21395
Position (kb) 4476
4477
4478 Strain fitness (log2 ratio) -1
0
1 at 4475.409 kb on - strand at 4475.499 kb on + strand at 4475.796 kb on - strand, within BWI76_RS21380 at 4475.819 kb on - strand, within BWI76_RS21380 at 4476.449 kb on - strand at 4476.457 kb on - strand at 4476.489 kb on - strand at 4476.489 kb on - strand at 4476.566 kb on + strand, within BWI76_RS21385 at 4476.574 kb on - strand, within BWI76_RS21385 at 4476.862 kb on - strand, within BWI76_RS21385 at 4477.005 kb on + strand, within BWI76_RS21385 at 4477.019 kb on - strand, within BWI76_RS21385 at 4477.205 kb on - strand, within BWI76_RS21385 at 4477.231 kb on - strand, within BWI76_RS21385 at 4477.368 kb on + strand at 4477.368 kb on + strand at 4477.376 kb on - strand at 4477.415 kb on + strand at 4477.566 kb on - strand at 4477.655 kb on - strand at 4477.689 kb on + strand at 4477.899 kb on + strand, within BWI76_RS21390 at 4477.936 kb on - strand, within BWI76_RS21390 at 4478.299 kb on - strand, within BWI76_RS21395
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 4,475,409 - +0.8 4,475,499 + -0.1 4,475,796 - BWI76_RS21380 0.23 -0.5 4,475,819 - BWI76_RS21380 0.26 -0.4 4,476,449 - -0.5 4,476,457 - +0.4 4,476,489 - -0.4 4,476,489 - +0.4 4,476,566 + BWI76_RS21385 0.17 -0.5 4,476,574 - BWI76_RS21385 0.18 +0.3 4,476,862 - BWI76_RS21385 0.44 +0.0 4,477,005 + BWI76_RS21385 0.58 +0.2 4,477,019 - BWI76_RS21385 0.59 +0.2 4,477,205 - BWI76_RS21385 0.76 +0.2 4,477,231 - BWI76_RS21385 0.79 +1.6 4,477,368 + -0.6 4,477,368 + -1.0 4,477,376 - +1.1 4,477,415 + -0.1 4,477,566 - +0.8 4,477,655 - +1.3 4,477,689 + +0.0 4,477,899 + BWI76_RS21390 0.56 +0.2 4,477,936 - BWI76_RS21390 0.64 +0.7 4,478,299 - BWI76_RS21395 0.20 +0.6
Or see this region's nucleotide sequence