Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20045 and BWI76_RS20050 are separated by 105 nucleotides BWI76_RS20050 and BWI76_RS20055 overlap by 24 nucleotides BWI76_RS20055 and BWI76_RS20060 overlap by 4 nucleotides BWI76_RS20060 and BWI76_RS20065 overlap by 1 nucleotides
BWI76_RS20045: BWI76_RS20045 - hypothetical protein, at 4,198,990 to 4,200,510
_RS20045
BWI76_RS20050: BWI76_RS20050 - hypothetical protein, at 4,200,616 to 4,201,239
_RS20050
BWI76_RS20055: BWI76_RS20055 - 2-succinylbenzoate-CoA ligase, at 4,201,216 to 4,202,607
_RS20055
BWI76_RS20060: BWI76_RS20060 - o-succinylbenzoate synthase, at 4,202,604 to 4,203,569
_RS20060
BWI76_RS20065: BWI76_RS20065 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,203,569 to 4,204,426
_RS20065
Position (kb)
4201
4202
4203 Strain fitness (log2 ratio)
-1
0
1 at 4200.844 kb on + strand, within BWI76_RS20050 at 4201.569 kb on - strand, within BWI76_RS20055 at 4201.754 kb on - strand, within BWI76_RS20055 at 4202.020 kb on - strand, within BWI76_RS20055 at 4202.519 kb on - strand at 4202.549 kb on + strand at 4202.602 kb on + strand at 4202.610 kb on - strand at 4202.632 kb on - strand at 4202.687 kb on + strand at 4202.728 kb on - strand, within BWI76_RS20060 at 4202.798 kb on + strand, within BWI76_RS20060 at 4202.935 kb on - strand, within BWI76_RS20060 at 4203.063 kb on - strand, within BWI76_RS20060 at 4203.151 kb on + strand, within BWI76_RS20060 at 4203.174 kb on - strand, within BWI76_RS20060 at 4203.306 kb on - strand, within BWI76_RS20060
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 4,200,844 + BWI76_RS20050 0.37 +0.2 4,201,569 - BWI76_RS20055 0.25 +0.7 4,201,754 - BWI76_RS20055 0.39 -0.0 4,202,020 - BWI76_RS20055 0.58 +0.3 4,202,519 - -0.1 4,202,549 + +0.1 4,202,602 + +0.0 4,202,610 - +0.2 4,202,632 - +0.3 4,202,687 + +0.3 4,202,728 - BWI76_RS20060 0.13 -0.1 4,202,798 + BWI76_RS20060 0.20 -1.0 4,202,935 - BWI76_RS20060 0.34 +0.4 4,203,063 - BWI76_RS20060 0.48 +0.0 4,203,151 + BWI76_RS20060 0.57 +0.3 4,203,174 - BWI76_RS20060 0.59 +0.3 4,203,306 - BWI76_RS20060 0.73 -0.8
Or see this region's nucleotide sequence