Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20055

Experiment: KLEB15 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20045 and BWI76_RS20050 are separated by 105 nucleotidesBWI76_RS20050 and BWI76_RS20055 overlap by 24 nucleotidesBWI76_RS20055 and BWI76_RS20060 overlap by 4 nucleotidesBWI76_RS20060 and BWI76_RS20065 overlap by 1 nucleotides BWI76_RS20045: BWI76_RS20045 - hypothetical protein, at 4,198,990 to 4,200,510 _RS20045 BWI76_RS20050: BWI76_RS20050 - hypothetical protein, at 4,200,616 to 4,201,239 _RS20050 BWI76_RS20055: BWI76_RS20055 - 2-succinylbenzoate-CoA ligase, at 4,201,216 to 4,202,607 _RS20055 BWI76_RS20060: BWI76_RS20060 - o-succinylbenzoate synthase, at 4,202,604 to 4,203,569 _RS20060 BWI76_RS20065: BWI76_RS20065 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,203,569 to 4,204,426 _RS20065 Position (kb) 4201 4202 4203Strain fitness (log2 ratio) -1 0 1at 4200.844 kb on + strand, within BWI76_RS20050at 4201.569 kb on - strand, within BWI76_RS20055at 4201.754 kb on - strand, within BWI76_RS20055at 4202.020 kb on - strand, within BWI76_RS20055at 4202.519 kb on - strandat 4202.549 kb on + strandat 4202.602 kb on + strandat 4202.610 kb on - strandat 4202.632 kb on - strandat 4202.687 kb on + strandat 4202.728 kb on - strand, within BWI76_RS20060at 4202.798 kb on + strand, within BWI76_RS20060at 4202.935 kb on - strand, within BWI76_RS20060at 4203.063 kb on - strand, within BWI76_RS20060at 4203.151 kb on + strand, within BWI76_RS20060at 4203.174 kb on - strand, within BWI76_RS20060at 4203.306 kb on - strand, within BWI76_RS20060

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 1
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4,200,844 + BWI76_RS20050 0.37 +0.2
4,201,569 - BWI76_RS20055 0.25 +0.7
4,201,754 - BWI76_RS20055 0.39 -0.0
4,202,020 - BWI76_RS20055 0.58 +0.3
4,202,519 - -0.1
4,202,549 + +0.1
4,202,602 + +0.0
4,202,610 - +0.2
4,202,632 - +0.3
4,202,687 + +0.3
4,202,728 - BWI76_RS20060 0.13 -0.1
4,202,798 + BWI76_RS20060 0.20 -1.0
4,202,935 - BWI76_RS20060 0.34 +0.4
4,203,063 - BWI76_RS20060 0.48 +0.0
4,203,151 + BWI76_RS20060 0.57 +0.3
4,203,174 - BWI76_RS20060 0.59 +0.3
4,203,306 - BWI76_RS20060 0.73 -0.8

Or see this region's nucleotide sequence