Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17320 and BWI76_RS17325 overlap by 1 nucleotides BWI76_RS17325 and BWI76_RS17330 overlap by 4 nucleotides BWI76_RS17330 and BWI76_RS17335 overlap by 4 nucleotides
BWI76_RS17320: BWI76_RS17320 - respiratory nitrate reductase subunit gamma, at 3,585,392 to 3,586,069
_RS17320
BWI76_RS17325: BWI76_RS17325 - nitrate reductase molybdenum cofactor assembly chaperone, at 3,586,069 to 3,586,779
_RS17325
BWI76_RS17330: BWI76_RS17330 - nitrate reductase subunit beta, at 3,586,776 to 3,588,311
_RS17330
BWI76_RS17335: BWI76_RS17335 - nitrate reductase subunit alpha, at 3,588,308 to 3,592,051
_RS17335
Position (kb)
3586
3587
3588
3589 Strain fitness (log2 ratio)
-1
0
1 at 3585.852 kb on + strand, within BWI76_RS17320 at 3585.976 kb on - strand, within BWI76_RS17320 at 3586.413 kb on + strand, within BWI76_RS17325 at 3586.423 kb on + strand, within BWI76_RS17325 at 3586.513 kb on - strand, within BWI76_RS17325 at 3586.573 kb on + strand, within BWI76_RS17325 at 3586.661 kb on - strand, within BWI76_RS17325 at 3587.002 kb on + strand, within BWI76_RS17330 at 3587.251 kb on + strand, within BWI76_RS17330 at 3587.444 kb on - strand, within BWI76_RS17330 at 3587.501 kb on + strand, within BWI76_RS17330 at 3588.428 kb on + strand at 3588.483 kb on - strand at 3588.523 kb on + strand at 3588.872 kb on - strand, within BWI76_RS17335 at 3589.007 kb on - strand, within BWI76_RS17335 at 3589.276 kb on - strand, within BWI76_RS17335 at 3589.288 kb on + strand, within BWI76_RS17335
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 3,585,852 + BWI76_RS17320 0.68 +0.1 3,585,976 - BWI76_RS17320 0.86 +0.1 3,586,413 + BWI76_RS17325 0.48 -0.6 3,586,423 + BWI76_RS17325 0.50 +0.2 3,586,513 - BWI76_RS17325 0.62 -0.0 3,586,573 + BWI76_RS17325 0.71 -0.2 3,586,661 - BWI76_RS17325 0.83 -0.6 3,587,002 + BWI76_RS17330 0.15 +1.3 3,587,251 + BWI76_RS17330 0.31 -0.0 3,587,444 - BWI76_RS17330 0.43 +0.4 3,587,501 + BWI76_RS17330 0.47 +0.8 3,588,428 + +0.0 3,588,483 - -0.5 3,588,523 + +0.5 3,588,872 - BWI76_RS17335 0.15 -0.4 3,589,007 - BWI76_RS17335 0.19 -1.0 3,589,276 - BWI76_RS17335 0.26 -0.1 3,589,288 + BWI76_RS17335 0.26 +0.7
Or see this region's nucleotide sequence