Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15200 and BWI76_RS15205 are separated by 204 nucleotides BWI76_RS15205 and BWI76_RS15210 are separated by 105 nucleotides BWI76_RS15210 and BWI76_RS15215 overlap by 47 nucleotides
BWI76_RS15200: BWI76_RS15200 - glutaminase, at 3,186,012 to 3,186,938
_RS15200
BWI76_RS15205: BWI76_RS15205 - succinate-semialdehyde dehydrogenase, at 3,187,143 to 3,188,528
_RS15205
BWI76_RS15210: BWI76_RS15210 - LysR family transcriptional regulator, at 3,188,634 to 3,189,512
_RS15210
BWI76_RS15215: BWI76_RS15215 - N-acetyltransferase, at 3,189,466 to 3,189,948
_RS15215
Position (kb)
3187
3188
3189 Strain fitness (log2 ratio)
-1
0
1 at 3186.326 kb on + strand, within BWI76_RS15200 at 3186.539 kb on - strand, within BWI76_RS15200 at 3187.198 kb on - strand at 3187.473 kb on + strand, within BWI76_RS15205 at 3187.555 kb on - strand, within BWI76_RS15205 at 3188.031 kb on + strand, within BWI76_RS15205 at 3188.691 kb on - strand at 3188.970 kb on - strand, within BWI76_RS15210 at 3189.178 kb on + strand, within BWI76_RS15210 at 3189.322 kb on + strand, within BWI76_RS15210 at 3189.351 kb on - strand, within BWI76_RS15210
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 3,186,326 + BWI76_RS15200 0.34 +0.2 3,186,539 - BWI76_RS15200 0.57 -0.5 3,187,198 - -0.2 3,187,473 + BWI76_RS15205 0.24 +0.8 3,187,555 - BWI76_RS15205 0.30 +0.3 3,188,031 + BWI76_RS15205 0.64 +0.7 3,188,691 - -0.6 3,188,970 - BWI76_RS15210 0.38 -0.7 3,189,178 + BWI76_RS15210 0.62 -0.3 3,189,322 + BWI76_RS15210 0.78 -0.9 3,189,351 - BWI76_RS15210 0.82 +0.8
Or see this region's nucleotide sequence