Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14275 and BWI76_RS14280 are separated by 74 nucleotides BWI76_RS14280 and BWI76_RS14285 are separated by 310 nucleotides BWI76_RS14285 and BWI76_RS14290 are separated by 42 nucleotides BWI76_RS14290 and BWI76_RS14295 are separated by 370 nucleotides
BWI76_RS14275: BWI76_RS14275 - hypothetical protein, at 2,985,593 to 2,986,789
_RS14275
BWI76_RS14280: BWI76_RS14280 - hypothetical protein, at 2,986,864 to 2,987,121
_RS14280
BWI76_RS14285: BWI76_RS14285 - IS3 family transposase, at 2,987,432 to 2,987,722
_RS14285
BWI76_RS14290: BWI76_RS14290 - AAA family ATPase, at 2,987,765 to 2,988,368
_RS14290
BWI76_RS14295: BWI76_RS14295 - IS630 family transposase, at 2,988,739 to 2,989,680
_RS14295
Position (kb)
2987
2988
2989 Strain fitness (log2 ratio)
-2
-1
0
1 at 2986.839 kb on - strand at 2986.875 kb on + strand at 2986.942 kb on + strand, within BWI76_RS14280 at 2986.993 kb on + strand, within BWI76_RS14280 at 2987.043 kb on - strand, within BWI76_RS14280 at 2987.173 kb on + strand at 2987.185 kb on - strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.250 kb on - strand at 2987.275 kb on + strand at 2987.276 kb on + strand at 2987.293 kb on - strand at 2987.401 kb on + strand at 2987.446 kb on + strand at 2987.481 kb on - strand, within BWI76_RS14285 at 2987.491 kb on - strand, within BWI76_RS14285 at 2987.514 kb on - strand, within BWI76_RS14285 at 2987.520 kb on + strand, within BWI76_RS14285 at 2987.524 kb on + strand, within BWI76_RS14285 at 2987.534 kb on + strand, within BWI76_RS14285 at 2987.534 kb on + strand, within BWI76_RS14285 at 2987.878 kb on + strand, within BWI76_RS14290 at 2987.886 kb on - strand, within BWI76_RS14290 at 2987.898 kb on + strand, within BWI76_RS14290 at 2987.921 kb on + strand, within BWI76_RS14290 at 2987.955 kb on + strand, within BWI76_RS14290 at 2988.029 kb on + strand, within BWI76_RS14290 at 2988.079 kb on + strand, within BWI76_RS14290 at 2988.231 kb on - strand, within BWI76_RS14290 at 2988.344 kb on + strand at 2988.463 kb on - strand at 2988.472 kb on - strand at 2988.516 kb on + strand at 2988.531 kb on - strand at 2988.586 kb on - strand at 2988.596 kb on - strand at 2988.621 kb on - strand at 2988.808 kb on + strand at 2988.840 kb on + strand, within BWI76_RS14295 at 2988.999 kb on - strand, within BWI76_RS14295 at 2989.053 kb on - strand, within BWI76_RS14295 at 2989.125 kb on - strand, within BWI76_RS14295 at 2989.275 kb on + strand, within BWI76_RS14295 at 2989.275 kb on + strand, within BWI76_RS14295 at 2989.275 kb on + strand, within BWI76_RS14295 at 2989.283 kb on - strand, within BWI76_RS14295 at 2989.283 kb on - strand, within BWI76_RS14295 at 2989.286 kb on + strand, within BWI76_RS14295 at 2989.294 kb on - strand, within BWI76_RS14295
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 2,986,839 - -0.9 2,986,875 + -0.7 2,986,942 + BWI76_RS14280 0.30 -0.1 2,986,993 + BWI76_RS14280 0.50 +0.2 2,987,043 - BWI76_RS14280 0.69 -0.4 2,987,173 + +1.6 2,987,185 - +0.0 2,987,194 + -0.8 2,987,194 + -0.1 2,987,194 + +0.2 2,987,194 + -0.0 2,987,194 + -0.2 2,987,194 + -0.4 2,987,194 + -0.3 2,987,194 + -0.5 2,987,194 + -0.6 2,987,194 + -0.0 2,987,194 + +0.3 2,987,194 + +0.0 2,987,194 + +0.2 2,987,194 + -0.3 2,987,194 + +0.2 2,987,202 - -1.0 2,987,202 - +0.3 2,987,202 - -0.9 2,987,202 - +0.3 2,987,202 - -1.5 2,987,202 - -0.1 2,987,202 - +0.6 2,987,202 - +0.1 2,987,202 - -1.3 2,987,202 - -0.6 2,987,202 - -0.7 2,987,250 - -0.9 2,987,275 + +0.3 2,987,276 + -0.3 2,987,293 - -0.4 2,987,401 + -0.1 2,987,446 + -0.2 2,987,481 - BWI76_RS14285 0.17 +0.9 2,987,491 - BWI76_RS14285 0.20 +0.0 2,987,514 - BWI76_RS14285 0.28 -0.4 2,987,520 + BWI76_RS14285 0.30 +0.4 2,987,524 + BWI76_RS14285 0.32 +0.0 2,987,534 + BWI76_RS14285 0.35 +0.2 2,987,534 + BWI76_RS14285 0.35 +0.4 2,987,878 + BWI76_RS14290 0.19 -1.2 2,987,886 - BWI76_RS14290 0.20 -0.2 2,987,898 + BWI76_RS14290 0.22 +1.4 2,987,921 + BWI76_RS14290 0.26 -0.3 2,987,955 + BWI76_RS14290 0.31 -0.7 2,988,029 + BWI76_RS14290 0.44 -2.2 2,988,079 + BWI76_RS14290 0.52 -0.7 2,988,231 - BWI76_RS14290 0.77 -0.4 2,988,344 + +0.3 2,988,463 - +0.4 2,988,472 - -0.7 2,988,516 + -0.3 2,988,531 - -0.1 2,988,586 - -0.2 2,988,596 - -0.3 2,988,621 - +0.4 2,988,808 + +0.6 2,988,840 + BWI76_RS14295 0.11 +0.7 2,988,999 - BWI76_RS14295 0.28 -0.0 2,989,053 - BWI76_RS14295 0.33 -0.5 2,989,125 - BWI76_RS14295 0.41 -0.8 2,989,275 + BWI76_RS14295 0.57 -1.1 2,989,275 + BWI76_RS14295 0.57 +0.3 2,989,275 + BWI76_RS14295 0.57 -0.2 2,989,283 - BWI76_RS14295 0.58 -0.3 2,989,283 - BWI76_RS14295 0.58 -1.4 2,989,286 + BWI76_RS14295 0.58 +0.1 2,989,294 - BWI76_RS14295 0.59 -0.6
Or see this region's nucleotide sequence