Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11355 and BWI76_RS11360 are separated by 278 nucleotides BWI76_RS11360 and BWI76_RS11365 are separated by 36 nucleotides BWI76_RS11365 and BWI76_RS11370 are separated by 0 nucleotides
BWI76_RS11355: BWI76_RS11355 - phosphodiesterase, at 2,392,835 to 2,394,022
_RS11355
BWI76_RS11360: BWI76_RS11360 - L-asparaginase, at 2,394,301 to 2,395,344
_RS11360
BWI76_RS11365: BWI76_RS11365 - iron ABC transporter, at 2,395,381 to 2,396,148
_RS11365
BWI76_RS11370: BWI76_RS11370 - iron-dicitrate transporter subunit FecD, at 2,396,149 to 2,397,102
_RS11370
Position (kb)
2394
2395
2396 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2393.352 kb on - strand, within BWI76_RS11355 at 2393.354 kb on + strand, within BWI76_RS11355 at 2393.362 kb on + strand, within BWI76_RS11355 at 2393.362 kb on + strand, within BWI76_RS11355 at 2393.535 kb on + strand, within BWI76_RS11355 at 2393.543 kb on - strand, within BWI76_RS11355 at 2393.679 kb on - strand, within BWI76_RS11355 at 2393.776 kb on + strand, within BWI76_RS11355 at 2393.898 kb on + strand, within BWI76_RS11355 at 2393.898 kb on + strand, within BWI76_RS11355 at 2393.932 kb on - strand at 2393.953 kb on - strand at 2394.058 kb on - strand at 2394.092 kb on - strand at 2394.128 kb on - strand at 2394.143 kb on + strand at 2394.166 kb on + strand at 2394.477 kb on + strand, within BWI76_RS11360 at 2394.477 kb on + strand, within BWI76_RS11360 at 2394.485 kb on - strand, within BWI76_RS11360 at 2394.498 kb on + strand, within BWI76_RS11360 at 2394.598 kb on - strand, within BWI76_RS11360 at 2395.329 kb on + strand at 2395.541 kb on + strand, within BWI76_RS11365 at 2395.551 kb on + strand, within BWI76_RS11365 at 2395.784 kb on - strand, within BWI76_RS11365 at 2396.128 kb on - strand at 2396.177 kb on + strand at 2396.210 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 2,393,352 - BWI76_RS11355 0.44 +0.6 2,393,354 + BWI76_RS11355 0.44 -0.3 2,393,362 + BWI76_RS11355 0.44 -0.2 2,393,362 + BWI76_RS11355 0.44 -0.7 2,393,535 + BWI76_RS11355 0.59 +0.2 2,393,543 - BWI76_RS11355 0.60 -0.1 2,393,679 - BWI76_RS11355 0.71 +0.9 2,393,776 + BWI76_RS11355 0.79 -0.2 2,393,898 + BWI76_RS11355 0.89 -0.3 2,393,898 + BWI76_RS11355 0.89 +0.4 2,393,932 - +1.8 2,393,953 - +0.7 2,394,058 - -0.4 2,394,092 - -0.3 2,394,128 - -0.1 2,394,143 + +0.4 2,394,166 + +1.8 2,394,477 + BWI76_RS11360 0.17 -0.4 2,394,477 + BWI76_RS11360 0.17 -2.7 2,394,485 - BWI76_RS11360 0.18 -0.7 2,394,498 + BWI76_RS11360 0.19 -1.1 2,394,598 - BWI76_RS11360 0.28 -0.6 2,395,329 + -0.4 2,395,541 + BWI76_RS11365 0.21 +0.3 2,395,551 + BWI76_RS11365 0.22 +0.4 2,395,784 - BWI76_RS11365 0.52 -0.1 2,396,128 - -0.6 2,396,177 + +0.1 2,396,210 + +0.1
Or see this region's nucleotide sequence