Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10425 and BWI76_RS10430 are separated by 107 nucleotides BWI76_RS10430 and BWI76_RS10435 are separated by 44 nucleotides BWI76_RS10435 and BWI76_RS10440 are separated by 7 nucleotides
BWI76_RS10425: BWI76_RS10425 - AIG2 family protein, at 2,204,434 to 2,204,769
_RS10425
BWI76_RS10430: BWI76_RS10430 - thiol:disulfide interchange protein DsbG, at 2,204,877 to 2,205,635
_RS10430
BWI76_RS10435: BWI76_RS10435 - suppressor for copper-sensitivity C, at 2,205,680 to 2,206,402
_RS10435
BWI76_RS10440: BWI76_RS10440 - protein-disulfide reductase, at 2,206,410 to 2,208,434
_RS10440
Position (kb)
2205
2206
2207 Strain fitness (log2 ratio)
-1
0
1 at 2204.744 kb on - strand at 2205.247 kb on + strand, within BWI76_RS10430 at 2205.360 kb on + strand, within BWI76_RS10430 at 2205.445 kb on + strand, within BWI76_RS10430 at 2205.491 kb on - strand, within BWI76_RS10430 at 2205.589 kb on - strand at 2205.739 kb on - strand at 2205.802 kb on + strand, within BWI76_RS10435 at 2206.100 kb on + strand, within BWI76_RS10435 at 2206.108 kb on - strand, within BWI76_RS10435 at 2206.505 kb on + strand at 2206.705 kb on + strand, within BWI76_RS10440 at 2206.789 kb on + strand, within BWI76_RS10440 at 2207.397 kb on - strand, within BWI76_RS10440
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 2,204,744 - -0.0 2,205,247 + BWI76_RS10430 0.49 -0.2 2,205,360 + BWI76_RS10430 0.64 -0.2 2,205,445 + BWI76_RS10430 0.75 -0.2 2,205,491 - BWI76_RS10430 0.81 +0.1 2,205,589 - +0.2 2,205,739 - -0.0 2,205,802 + BWI76_RS10435 0.17 -1.1 2,206,100 + BWI76_RS10435 0.58 -0.6 2,206,108 - BWI76_RS10435 0.59 -0.2 2,206,505 + -0.6 2,206,705 + BWI76_RS10440 0.15 +0.2 2,206,789 + BWI76_RS10440 0.19 -0.2 2,207,397 - BWI76_RS10440 0.49 +0.4
Or see this region's nucleotide sequence