Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08975 and BWI76_RS08980 are separated by 376 nucleotides BWI76_RS08980 and BWI76_RS08985 are separated by 221 nucleotides
BWI76_RS08975: BWI76_RS08975 - amino acid ABC transporter substrate-binding protein, at 1,891,396 to 1,892,142
_RS08975
BWI76_RS08980: BWI76_RS08980 - DNA starvation/stationary phase protection protein, at 1,892,519 to 1,893,022
_RS08980
BWI76_RS08985: BWI76_RS08985 - threonine/homoserine exporter RhtA, at 1,893,244 to 1,894,131
_RS08985
Position (kb)
1892
1893
1894 Strain fitness (log2 ratio)
-1
0
1 at 1891.553 kb on + strand, within BWI76_RS08975 at 1891.944 kb on + strand, within BWI76_RS08975 at 1891.981 kb on + strand, within BWI76_RS08975 at 1891.994 kb on - strand, within BWI76_RS08975 at 1892.126 kb on - strand at 1892.145 kb on - strand at 1892.167 kb on + strand at 1892.167 kb on + strand at 1892.449 kb on + strand at 1892.457 kb on - strand at 1892.524 kb on - strand at 1892.683 kb on - strand, within BWI76_RS08980 at 1893.108 kb on + strand at 1893.299 kb on - strand at 1893.461 kb on + strand, within BWI76_RS08985 at 1893.469 kb on - strand, within BWI76_RS08985 at 1893.586 kb on + strand, within BWI76_RS08985 at 1893.950 kb on + strand, within BWI76_RS08985
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 1,891,553 + BWI76_RS08975 0.21 -0.2 1,891,944 + BWI76_RS08975 0.73 -0.5 1,891,981 + BWI76_RS08975 0.78 -1.0 1,891,994 - BWI76_RS08975 0.80 -0.5 1,892,126 - +0.3 1,892,145 - -0.1 1,892,167 + +0.2 1,892,167 + -0.4 1,892,449 + +1.5 1,892,457 - -1.0 1,892,524 - -0.9 1,892,683 - BWI76_RS08980 0.33 +0.7 1,893,108 + +0.3 1,893,299 - +0.1 1,893,461 + BWI76_RS08985 0.24 -0.2 1,893,469 - BWI76_RS08985 0.25 +0.1 1,893,586 + BWI76_RS08985 0.39 -1.0 1,893,950 + BWI76_RS08985 0.80 -0.0
Or see this region's nucleotide sequence