Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01365

Experiment: KLEB15 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01360 and BWI76_RS01365 are separated by 9 nucleotidesBWI76_RS01365 and BWI76_RS01370 are separated by 1 nucleotidesBWI76_RS01370 and BWI76_RS01375 are separated by 64 nucleotides BWI76_RS01360: BWI76_RS01360 - acetyl-CoA C-acyltransferase FadA, at 277,464 to 278,627 _RS01360 BWI76_RS01365: BWI76_RS01365 - multifunctional fatty acid oxidation complex subunit alpha, at 278,637 to 280,826 _RS01365 BWI76_RS01370: BWI76_RS01370 - 3-hydroxyacyl-CoA dehydrogenase, at 280,828 to 280,953 _RS01370 BWI76_RS01375: BWI76_RS01375 - Xaa-Pro dipeptidase, at 281,018 to 282,349 _RS01375 Position (kb) 278 279 280 281Strain fitness (log2 ratio) -1 0 1 2 3at 277.652 kb on - strand, within BWI76_RS01360at 277.814 kb on - strand, within BWI76_RS01360at 277.907 kb on + strand, within BWI76_RS01360at 277.933 kb on + strand, within BWI76_RS01360at 278.006 kb on + strand, within BWI76_RS01360at 278.022 kb on + strand, within BWI76_RS01360at 278.103 kb on - strand, within BWI76_RS01360at 278.137 kb on - strand, within BWI76_RS01360at 278.446 kb on - strand, within BWI76_RS01360at 278.446 kb on - strand, within BWI76_RS01360at 278.446 kb on - strand, within BWI76_RS01360at 278.803 kb on + strandat 278.894 kb on + strand, within BWI76_RS01365at 278.979 kb on + strand, within BWI76_RS01365at 279.102 kb on - strand, within BWI76_RS01365at 279.293 kb on + strand, within BWI76_RS01365at 279.475 kb on - strand, within BWI76_RS01365at 279.514 kb on - strand, within BWI76_RS01365at 279.546 kb on + strand, within BWI76_RS01365at 279.677 kb on - strand, within BWI76_RS01365at 279.677 kb on - strand, within BWI76_RS01365at 279.715 kb on - strand, within BWI76_RS01365at 279.752 kb on - strand, within BWI76_RS01365at 279.765 kb on + strand, within BWI76_RS01365at 279.766 kb on + strand, within BWI76_RS01365at 280.157 kb on + strand, within BWI76_RS01365at 280.192 kb on + strand, within BWI76_RS01365at 280.298 kb on + strand, within BWI76_RS01365at 280.298 kb on + strand, within BWI76_RS01365at 280.311 kb on + strand, within BWI76_RS01365at 280.319 kb on - strand, within BWI76_RS01365at 280.485 kb on - strand, within BWI76_RS01365at 280.495 kb on - strand, within BWI76_RS01365at 280.583 kb on - strand, within BWI76_RS01365at 280.601 kb on - strand, within BWI76_RS01365at 280.690 kb on + strandat 280.710 kb on - strandat 280.724 kb on - strandat 280.805 kb on + strandat 280.951 kb on - strandat 280.971 kb on - strandat 281.092 kb on + strandat 281.121 kb on + strandat 281.121 kb on + strandat 281.204 kb on + strand, within BWI76_RS01375at 281.289 kb on + strand, within BWI76_RS01375at 281.342 kb on - strand, within BWI76_RS01375at 281.457 kb on + strand, within BWI76_RS01375at 281.508 kb on + strand, within BWI76_RS01375at 281.656 kb on - strand, within BWI76_RS01375at 281.686 kb on + strand, within BWI76_RS01375at 281.698 kb on + strand, within BWI76_RS01375at 281.711 kb on - strand, within BWI76_RS01375at 281.737 kb on + strand, within BWI76_RS01375

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 1
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277,652 - BWI76_RS01360 0.16 +0.1
277,814 - BWI76_RS01360 0.30 +0.2
277,907 + BWI76_RS01360 0.38 +1.4
277,933 + BWI76_RS01360 0.40 +0.0
278,006 + BWI76_RS01360 0.47 +1.0
278,022 + BWI76_RS01360 0.48 +0.3
278,103 - BWI76_RS01360 0.55 +0.3
278,137 - BWI76_RS01360 0.58 +0.9
278,446 - BWI76_RS01360 0.84 -1.0
278,446 - BWI76_RS01360 0.84 +0.8
278,446 - BWI76_RS01360 0.84 +0.3
278,803 + +0.0
278,894 + BWI76_RS01365 0.12 -0.1
278,979 + BWI76_RS01365 0.16 +0.1
279,102 - BWI76_RS01365 0.21 +0.7
279,293 + BWI76_RS01365 0.30 +0.7
279,475 - BWI76_RS01365 0.38 +0.4
279,514 - BWI76_RS01365 0.40 +0.0
279,546 + BWI76_RS01365 0.42 +0.3
279,677 - BWI76_RS01365 0.47 +0.0
279,677 - BWI76_RS01365 0.47 -0.0
279,715 - BWI76_RS01365 0.49 -0.2
279,752 - BWI76_RS01365 0.51 -0.8
279,765 + BWI76_RS01365 0.52 +0.4
279,766 + BWI76_RS01365 0.52 +0.7
280,157 + BWI76_RS01365 0.69 +0.8
280,192 + BWI76_RS01365 0.71 +0.3
280,298 + BWI76_RS01365 0.76 +0.8
280,298 + BWI76_RS01365 0.76 +1.0
280,311 + BWI76_RS01365 0.76 +0.3
280,319 - BWI76_RS01365 0.77 +0.2
280,485 - BWI76_RS01365 0.84 +0.9
280,495 - BWI76_RS01365 0.85 +0.4
280,583 - BWI76_RS01365 0.89 +3.6
280,601 - BWI76_RS01365 0.90 +0.6
280,690 + +0.6
280,710 - +0.7
280,724 - +1.4
280,805 + -0.4
280,951 - +0.7
280,971 - +0.3
281,092 + +0.5
281,121 + +0.2
281,121 + -0.8
281,204 + BWI76_RS01375 0.14 -0.2
281,289 + BWI76_RS01375 0.20 +0.3
281,342 - BWI76_RS01375 0.24 +0.7
281,457 + BWI76_RS01375 0.33 +0.3
281,508 + BWI76_RS01375 0.37 -0.0
281,656 - BWI76_RS01375 0.48 -0.0
281,686 + BWI76_RS01375 0.50 +0.2
281,698 + BWI76_RS01375 0.51 +0.0
281,711 - BWI76_RS01375 0.52 -0.8
281,737 + BWI76_RS01375 0.54 +0.3

Or see this region's nucleotide sequence