Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16140

Experiment: No phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16135 and BWI76_RS16140 are separated by 10 nucleotidesBWI76_RS16140 and BWI76_RS16145 are separated by 10 nucleotidesBWI76_RS16145 and BWI76_RS16150 are separated by 167 nucleotides BWI76_RS16135: BWI76_RS16135 - acetyl-CoA acetyltransferase, at 3,347,750 to 3,348,952 _RS16135 BWI76_RS16140: BWI76_RS16140 - 3-oxoadipate CoA-transferase, at 3,348,963 to 3,349,619 _RS16140 BWI76_RS16145: BWI76_RS16145 - 3-oxoadipate CoA-transferase, at 3,349,630 to 3,350,316 _RS16145 BWI76_RS16150: BWI76_RS16150 - IclR family transcriptional regulator, at 3,350,484 to 3,351,290 _RS16150 Position (kb) 3348 3349 3350Strain fitness (log2 ratio) -1 0 1at 3348.604 kb on - strand, within BWI76_RS16135at 3349.004 kb on + strandat 3349.401 kb on - strand, within BWI76_RS16140at 3349.411 kb on + strand, within BWI76_RS16140at 3350.569 kb on + strand, within BWI76_RS16150at 3350.577 kb on - strand, within BWI76_RS16150

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Per-strain Table

Position Strand Gene LocusTag Fraction No phage control
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3,348,604 - BWI76_RS16135 0.71 +1.2
3,349,004 + -0.5
3,349,401 - BWI76_RS16140 0.67 -0.8
3,349,411 + BWI76_RS16140 0.68 -0.1
3,350,569 + BWI76_RS16150 0.11 -0.2
3,350,577 - BWI76_RS16150 0.12 -0.0

Or see this region's nucleotide sequence