Experiment: No phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03840 and BWI76_RS03845 are separated by 70 nucleotides BWI76_RS03845 and BWI76_RS03850 are separated by 10 nucleotides BWI76_RS03850 and BWI76_RS03855 are separated by 136 nucleotides
BWI76_RS03840: BWI76_RS03840 - 4-hydroxyphenylacetate permease, at 794,732 to 796,108
_RS03840
BWI76_RS03845: BWI76_RS03845 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase, at 796,179 to 796,971
_RS03845
BWI76_RS03850: BWI76_RS03850 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 796,982 to 797,785
_RS03850
BWI76_RS03855: BWI76_RS03855 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 797,922 to 798,302
_RS03855
Position (kb) 796
797 Strain fitness (log2 ratio) -1
0
1 at 795.315 kb on + strand, within BWI76_RS03840 at 795.351 kb on - strand, within BWI76_RS03840 at 795.656 kb on - strand, within BWI76_RS03840 at 795.700 kb on + strand, within BWI76_RS03840 at 795.724 kb on + strand, within BWI76_RS03840 at 795.745 kb on - strand, within BWI76_RS03840 at 795.875 kb on - strand, within BWI76_RS03840 at 795.875 kb on - strand, within BWI76_RS03840 at 796.106 kb on + strand at 796.161 kb on + strand at 796.299 kb on + strand, within BWI76_RS03845 at 796.307 kb on - strand, within BWI76_RS03845 at 796.401 kb on + strand, within BWI76_RS03845 at 796.553 kb on + strand, within BWI76_RS03845 at 796.780 kb on + strand, within BWI76_RS03845 at 796.949 kb on - strand at 797.175 kb on - strand, within BWI76_RS03850 at 797.241 kb on - strand, within BWI76_RS03850 at 797.440 kb on - strand, within BWI76_RS03850 at 797.544 kb on + strand, within BWI76_RS03850 at 797.552 kb on - strand at 797.769 kb on - strand at 797.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No phage control remove 795,315 + BWI76_RS03840 0.42 -0.3 795,351 - BWI76_RS03840 0.45 -0.0 795,656 - BWI76_RS03840 0.67 +0.2 795,700 + BWI76_RS03840 0.70 -0.1 795,724 + BWI76_RS03840 0.72 +0.0 795,745 - BWI76_RS03840 0.74 -0.0 795,875 - BWI76_RS03840 0.83 -0.9 795,875 - BWI76_RS03840 0.83 -0.2 796,106 + -0.1 796,161 + -0.3 796,299 + BWI76_RS03845 0.15 -0.2 796,307 - BWI76_RS03845 0.16 -0.8 796,401 + BWI76_RS03845 0.28 +0.2 796,553 + BWI76_RS03845 0.47 -0.8 796,780 + BWI76_RS03845 0.76 -0.6 796,949 - +0.8 797,175 - BWI76_RS03850 0.24 +0.1 797,241 - BWI76_RS03850 0.32 +0.5 797,440 - BWI76_RS03850 0.57 +0.9 797,544 + BWI76_RS03850 0.70 -0.1 797,552 - +1.7 797,769 - -0.3 797,849 - +0.2
Or see this region's nucleotide sequence