Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00565

Experiment: No phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00555 and BWI76_RS00560 overlap by 4 nucleotidesBWI76_RS00560 and BWI76_RS00565 are separated by 90 nucleotidesBWI76_RS00565 and BWI76_RS00570 overlap by 1 nucleotidesBWI76_RS00570 and BWI76_RS00575 are separated by 100 nucleotides BWI76_RS00555: BWI76_RS00555 - PTS transporter subunit IIA-like nitrogen-regulatory protein PtsN, at 112,748 to 113,185 _RS00555 BWI76_RS00560: BWI76_RS00560 - hypothetical protein, at 113,182 to 113,736 _RS00560 BWI76_RS00565: BWI76_RS00565 - branched-chain amino acid transport, at 113,827 to 114,150 _RS00565 BWI76_RS00570: BWI76_RS00570 - AzlC family protein, at 114,150 to 114,809 _RS00570 BWI76_RS00575: BWI76_RS00575 - DNA-binding protein, at 114,910 to 115,458 _RS00575 Position (kb) 113 114 115Strain fitness (log2 ratio) -1 0 1at 112.856 kb on + strand, within BWI76_RS00555at 112.864 kb on - strand, within BWI76_RS00555at 112.864 kb on - strand, within BWI76_RS00555at 112.906 kb on - strand, within BWI76_RS00555at 112.906 kb on - strand, within BWI76_RS00555at 112.956 kb on + strand, within BWI76_RS00555at 112.983 kb on + strand, within BWI76_RS00555at 112.983 kb on + strand, within BWI76_RS00555at 112.983 kb on + strand, within BWI76_RS00555at 112.990 kb on - strand, within BWI76_RS00555at 112.991 kb on - strand, within BWI76_RS00555at 112.991 kb on - strand, within BWI76_RS00555at 112.991 kb on - strand, within BWI76_RS00555at 112.991 kb on - strand, within BWI76_RS00555at 112.991 kb on - strand, within BWI76_RS00555at 112.991 kb on - strand, within BWI76_RS00555at 112.991 kb on - strand, within BWI76_RS00555at 113.073 kb on - strand, within BWI76_RS00555at 113.073 kb on - strand, within BWI76_RS00555at 113.079 kb on + strand, within BWI76_RS00555at 113.110 kb on - strand, within BWI76_RS00555at 113.157 kb on + strandat 113.157 kb on + strandat 113.165 kb on - strandat 113.165 kb on - strandat 113.168 kb on - strandat 113.179 kb on + strandat 113.179 kb on + strandat 113.187 kb on - strandat 113.193 kb on + strandat 113.309 kb on + strand, within BWI76_RS00560at 113.346 kb on + strand, within BWI76_RS00560at 113.540 kb on + strand, within BWI76_RS00560at 113.590 kb on - strand, within BWI76_RS00560at 113.686 kb on + strandat 113.702 kb on - strandat 113.970 kb on + strand, within BWI76_RS00565at 113.970 kb on + strand, within BWI76_RS00565at 113.996 kb on + strand, within BWI76_RS00565at 114.014 kb on - strand, within BWI76_RS00565at 114.032 kb on + strand, within BWI76_RS00565at 114.255 kb on + strand, within BWI76_RS00570at 114.255 kb on + strand, within BWI76_RS00570at 114.448 kb on - strand, within BWI76_RS00570at 114.461 kb on + strand, within BWI76_RS00570at 114.539 kb on + strand, within BWI76_RS00570at 114.547 kb on - strand, within BWI76_RS00570at 114.547 kb on - strand, within BWI76_RS00570at 114.547 kb on - strand, within BWI76_RS00570at 114.646 kb on - strand, within BWI76_RS00570at 114.679 kb on - strand, within BWI76_RS00570at 114.704 kb on - strand, within BWI76_RS00570at 114.718 kb on + strand, within BWI76_RS00570at 114.726 kb on - strand, within BWI76_RS00570at 114.761 kb on - strandat 114.775 kb on - strandat 114.911 kb on - strandat 114.916 kb on - strandat 114.967 kb on + strand, within BWI76_RS00575at 115.127 kb on - strand, within BWI76_RS00575

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Per-strain Table

Position Strand Gene LocusTag Fraction No phage control
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112,856 + BWI76_RS00555 0.25 +0.1
112,864 - BWI76_RS00555 0.26 +0.6
112,864 - BWI76_RS00555 0.26 -0.0
112,906 - BWI76_RS00555 0.36 -0.4
112,906 - BWI76_RS00555 0.36 -0.1
112,956 + BWI76_RS00555 0.47 +0.6
112,983 + BWI76_RS00555 0.54 +0.3
112,983 + BWI76_RS00555 0.54 +1.6
112,983 + BWI76_RS00555 0.54 +0.0
112,990 - BWI76_RS00555 0.55 +0.3
112,991 - BWI76_RS00555 0.55 -0.2
112,991 - BWI76_RS00555 0.55 -1.1
112,991 - BWI76_RS00555 0.55 -0.2
112,991 - BWI76_RS00555 0.55 +0.0
112,991 - BWI76_RS00555 0.55 -0.4
112,991 - BWI76_RS00555 0.55 +0.4
112,991 - BWI76_RS00555 0.55 -0.0
113,073 - BWI76_RS00555 0.74 +0.3
113,073 - BWI76_RS00555 0.74 +0.2
113,079 + BWI76_RS00555 0.76 +0.0
113,110 - BWI76_RS00555 0.83 -0.3
113,157 + -0.1
113,157 + +0.9
113,165 - -0.1
113,165 - -1.7
113,168 - -0.4
113,179 + -0.1
113,179 + -0.3
113,187 - +0.4
113,193 + -0.3
113,309 + BWI76_RS00560 0.23 +0.2
113,346 + BWI76_RS00560 0.30 +0.0
113,540 + BWI76_RS00560 0.65 +0.0
113,590 - BWI76_RS00560 0.74 -0.3
113,686 + -0.2
113,702 - -0.3
113,970 + BWI76_RS00565 0.44 -0.4
113,970 + BWI76_RS00565 0.44 -0.2
113,996 + BWI76_RS00565 0.52 -0.6
114,014 - BWI76_RS00565 0.58 -0.2
114,032 + BWI76_RS00565 0.63 -0.7
114,255 + BWI76_RS00570 0.16 -0.2
114,255 + BWI76_RS00570 0.16 +0.0
114,448 - BWI76_RS00570 0.45 -0.2
114,461 + BWI76_RS00570 0.47 -0.6
114,539 + BWI76_RS00570 0.59 +0.2
114,547 - BWI76_RS00570 0.60 +0.4
114,547 - BWI76_RS00570 0.60 -0.5
114,547 - BWI76_RS00570 0.60 +0.1
114,646 - BWI76_RS00570 0.75 +0.1
114,679 - BWI76_RS00570 0.80 -0.4
114,704 - BWI76_RS00570 0.84 -0.1
114,718 + BWI76_RS00570 0.86 +0.3
114,726 - BWI76_RS00570 0.87 -1.1
114,761 - +0.5
114,775 - +0.0
114,911 - -0.3
114,916 - -0.4
114,967 + BWI76_RS00575 0.10 +0.2
115,127 - BWI76_RS00575 0.40 -0.3

Or see this region's nucleotide sequence