Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00060

Experiment: No phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00050 and BWI76_RS00055 are separated by 133 nucleotidesBWI76_RS00055 and BWI76_RS00060 are separated by 57 nucleotidesBWI76_RS00060 and BWI76_RS00065 are separated by 147 nucleotides BWI76_RS00050: BWI76_RS00050 - tRNA modification GTPase, at 9,264 to 10,628 _RS00050 BWI76_RS00055: BWI76_RS00055 - N-acetyltransferase, at 10,762 to 11,169 _RS00055 BWI76_RS00060: BWI76_RS00060 - N-acetylneuraminate lyase, at 11,227 to 12,123 _RS00060 BWI76_RS00065: BWI76_RS00065 - MurR/RpiR family transcriptional regulator, at 12,271 to 13,152 _RS00065 Position (kb) 11 12 13Strain fitness (log2 ratio) -1 0 1at 10.715 kb on + strandat 10.715 kb on + strandat 10.723 kb on - strandat 10.723 kb on - strandat 10.759 kb on + strandat 10.767 kb on - strandat 11.006 kb on - strand, within BWI76_RS00055at 11.485 kb on + strand, within BWI76_RS00060at 11.485 kb on + strand, within BWI76_RS00060at 11.697 kb on - strand, within BWI76_RS00060at 11.935 kb on - strand, within BWI76_RS00060at 12.356 kb on - strandat 12.428 kb on + strand, within BWI76_RS00065at 12.436 kb on - strand, within BWI76_RS00065at 12.465 kb on - strand, within BWI76_RS00065at 12.527 kb on - strand, within BWI76_RS00065at 12.584 kb on + strand, within BWI76_RS00065at 12.584 kb on + strand, within BWI76_RS00065at 12.695 kb on - strand, within BWI76_RS00065at 12.749 kb on + strand, within BWI76_RS00065at 12.749 kb on + strand, within BWI76_RS00065at 12.822 kb on + strand, within BWI76_RS00065

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Per-strain Table

Position Strand Gene LocusTag Fraction No phage control
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10,715 + -0.3
10,715 + -0.7
10,723 - -0.3
10,723 - -0.0
10,759 + -0.2
10,767 - +0.2
11,006 - BWI76_RS00055 0.60 +0.4
11,485 + BWI76_RS00060 0.29 -0.5
11,485 + BWI76_RS00060 0.29 -0.7
11,697 - BWI76_RS00060 0.52 -0.7
11,935 - BWI76_RS00060 0.79 -0.2
12,356 - -1.2
12,428 + BWI76_RS00065 0.18 -0.6
12,436 - BWI76_RS00065 0.19 +0.2
12,465 - BWI76_RS00065 0.22 -1.3
12,527 - BWI76_RS00065 0.29 -0.0
12,584 + BWI76_RS00065 0.35 -1.4
12,584 + BWI76_RS00065 0.35 -0.5
12,695 - BWI76_RS00065 0.48 -1.6
12,749 + BWI76_RS00065 0.54 -1.2
12,749 + BWI76_RS00065 0.54 -0.5
12,822 + BWI76_RS00065 0.62 -0.3

Or see this region's nucleotide sequence