Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24495 and BWI76_RS24500 are separated by 9 nucleotides BWI76_RS24500 and BWI76_RS24505 are separated by 9 nucleotides BWI76_RS24505 and BWI76_RS24510 overlap by 8 nucleotides
BWI76_RS24495: BWI76_RS24495 - urease accessory protein, at 5,108,180 to 5,109,004
_RS24495
BWI76_RS24500: BWI76_RS24500 - urease subunit gamma, at 5,109,014 to 5,109,316
_RS24500
BWI76_RS24505: BWI76_RS24505 - urease subunit beta, at 5,109,326 to 5,109,646
_RS24505
BWI76_RS24510: BWI76_RS24510 - urease subunit alpha, at 5,109,639 to 5,111,342
_RS24510
Position (kb)
5109
5110 Strain fitness (log2 ratio)
-2
-1
0
1 at 5108.396 kb on - strand, within BWI76_RS24495 at 5108.498 kb on - strand, within BWI76_RS24495 at 5108.527 kb on + strand, within BWI76_RS24495 at 5108.603 kb on + strand, within BWI76_RS24495 at 5108.628 kb on - strand, within BWI76_RS24495 at 5108.693 kb on + strand, within BWI76_RS24495 at 5108.772 kb on - strand, within BWI76_RS24495 at 5108.837 kb on - strand, within BWI76_RS24495 at 5108.999 kb on + strand at 5109.225 kb on - strand, within BWI76_RS24500 at 5109.246 kb on + strand, within BWI76_RS24500 at 5109.264 kb on - strand, within BWI76_RS24500 at 5109.279 kb on + strand, within BWI76_RS24500 at 5109.365 kb on + strand, within BWI76_RS24505 at 5109.613 kb on + strand, within BWI76_RS24505 at 5110.422 kb on - strand, within BWI76_RS24510 at 5110.539 kb on + strand, within BWI76_RS24510 at 5110.552 kb on - strand, within BWI76_RS24510 at 5110.572 kb on + strand, within BWI76_RS24510 at 5110.631 kb on - strand, within BWI76_RS24510
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 5,108,396 - BWI76_RS24495 0.26 -1.8 5,108,498 - BWI76_RS24495 0.39 +1.2 5,108,527 + BWI76_RS24495 0.42 +0.2 5,108,603 + BWI76_RS24495 0.51 +0.8 5,108,628 - BWI76_RS24495 0.54 -0.3 5,108,693 + BWI76_RS24495 0.62 +0.8 5,108,772 - BWI76_RS24495 0.72 +0.3 5,108,837 - BWI76_RS24495 0.80 -0.5 5,108,999 + +0.9 5,109,225 - BWI76_RS24500 0.70 -0.1 5,109,246 + BWI76_RS24500 0.77 +0.6 5,109,264 - BWI76_RS24500 0.83 -0.7 5,109,279 + BWI76_RS24500 0.87 +1.3 5,109,365 + BWI76_RS24505 0.12 +0.4 5,109,613 + BWI76_RS24505 0.89 +0.8 5,110,422 - BWI76_RS24510 0.46 -0.1 5,110,539 + BWI76_RS24510 0.53 -0.0 5,110,552 - BWI76_RS24510 0.54 -0.2 5,110,572 + BWI76_RS24510 0.55 -0.6 5,110,631 - BWI76_RS24510 0.58 +0.1
Or see this region's nucleotide sequence