Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16615 and BWI76_RS16620 are separated by 198 nucleotides BWI76_RS16620 and BWI76_RS16625 are separated by 373 nucleotides BWI76_RS16625 and BWI76_RS16630 are separated by 18 nucleotides BWI76_RS16630 and BWI76_RS16635 are separated by 88 nucleotides
BWI76_RS16615: BWI76_RS16615 - FAD/NAD(P) binding domain-containing protein, at 3,439,840 to 3,441,450
_RS16615
BWI76_RS16620: BWI76_RS16620 - TIGR00156 family protein, at 3,441,649 to 3,442,041
_RS16620
BWI76_RS16625: BWI76_RS16625 - putative oxidoreductase, at 3,442,415 to 3,443,299
_RS16625
BWI76_RS16630: BWI76_RS16630 - MBL fold metallo-hydrolase, at 3,443,318 to 3,444,124
_RS16630
BWI76_RS16635: BWI76_RS16635 - carbonate dehydratase, at 3,444,213 to 3,444,848
_RS16635
Position (kb)
3442
3443
3444 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3441.590 kb on - strand at 3441.633 kb on + strand at 3441.721 kb on + strand, within BWI76_RS16620 at 3441.731 kb on + strand, within BWI76_RS16620 at 3441.764 kb on + strand, within BWI76_RS16620 at 3441.772 kb on - strand, within BWI76_RS16620 at 3441.774 kb on + strand, within BWI76_RS16620 at 3441.781 kb on + strand, within BWI76_RS16620 at 3441.782 kb on - strand, within BWI76_RS16620 at 3441.801 kb on + strand, within BWI76_RS16620 at 3441.833 kb on + strand, within BWI76_RS16620 at 3441.865 kb on + strand, within BWI76_RS16620 at 3441.888 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.890 kb on + strand, within BWI76_RS16620 at 3441.896 kb on - strand, within BWI76_RS16620 at 3441.898 kb on - strand, within BWI76_RS16620 at 3441.898 kb on - strand, within BWI76_RS16620 at 3441.898 kb on - strand, within BWI76_RS16620 at 3441.898 kb on - strand, within BWI76_RS16620 at 3441.973 kb on + strand, within BWI76_RS16620 at 3441.978 kb on + strand at 3442.049 kb on + strand at 3442.052 kb on - strand at 3442.052 kb on - strand at 3442.057 kb on - strand at 3442.058 kb on + strand at 3442.066 kb on - strand at 3442.215 kb on - strand at 3442.262 kb on - strand at 3442.385 kb on + strand at 3442.385 kb on + strand at 3442.408 kb on + strand at 3442.416 kb on - strand at 3442.416 kb on - strand at 3442.457 kb on - strand at 3442.526 kb on + strand, within BWI76_RS16625 at 3442.526 kb on + strand, within BWI76_RS16625 at 3442.592 kb on + strand, within BWI76_RS16625 at 3442.729 kb on + strand, within BWI76_RS16625 at 3442.776 kb on - strand, within BWI76_RS16625 at 3443.007 kb on + strand, within BWI76_RS16625 at 3443.062 kb on + strand, within BWI76_RS16625 at 3443.215 kb on + strand at 3443.399 kb on + strand, within BWI76_RS16630 at 3443.415 kb on + strand, within BWI76_RS16630 at 3443.588 kb on + strand, within BWI76_RS16630 at 3443.596 kb on - strand, within BWI76_RS16630 at 3443.596 kb on - strand, within BWI76_RS16630 at 3443.672 kb on - strand, within BWI76_RS16630 at 3443.779 kb on - strand, within BWI76_RS16630 at 3443.952 kb on + strand, within BWI76_RS16630 at 3444.163 kb on - strand at 3444.163 kb on - strand at 3444.163 kb on - strand at 3444.184 kb on + strand at 3444.202 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 3,441,590 - +0.3 3,441,633 + -1.1 3,441,721 + BWI76_RS16620 0.18 +2.5 3,441,731 + BWI76_RS16620 0.21 -0.0 3,441,764 + BWI76_RS16620 0.29 +0.1 3,441,772 - BWI76_RS16620 0.31 +0.9 3,441,774 + BWI76_RS16620 0.32 -2.3 3,441,781 + BWI76_RS16620 0.34 +1.1 3,441,782 - BWI76_RS16620 0.34 -0.4 3,441,801 + BWI76_RS16620 0.39 +0.7 3,441,833 + BWI76_RS16620 0.47 -0.6 3,441,865 + BWI76_RS16620 0.55 +0.5 3,441,888 + BWI76_RS16620 0.61 -0.4 3,441,890 + BWI76_RS16620 0.61 +1.9 3,441,890 + BWI76_RS16620 0.61 -0.7 3,441,890 + BWI76_RS16620 0.61 +0.9 3,441,890 + BWI76_RS16620 0.61 -2.5 3,441,890 + BWI76_RS16620 0.61 -0.2 3,441,890 + BWI76_RS16620 0.61 -0.5 3,441,890 + BWI76_RS16620 0.61 +0.6 3,441,890 + BWI76_RS16620 0.61 -1.4 3,441,890 + BWI76_RS16620 0.61 -0.5 3,441,890 + BWI76_RS16620 0.61 -0.7 3,441,890 + BWI76_RS16620 0.61 +1.5 3,441,896 - BWI76_RS16620 0.63 +1.0 3,441,898 - BWI76_RS16620 0.63 +2.1 3,441,898 - BWI76_RS16620 0.63 +0.3 3,441,898 - BWI76_RS16620 0.63 +1.5 3,441,898 - BWI76_RS16620 0.63 -1.3 3,441,973 + BWI76_RS16620 0.82 +0.4 3,441,978 + +0.7 3,442,049 + -0.9 3,442,052 - +0.5 3,442,052 - +0.6 3,442,057 - -2.1 3,442,058 + +0.1 3,442,066 - +0.9 3,442,215 - -0.3 3,442,262 - +0.2 3,442,385 + +1.9 3,442,385 + -0.5 3,442,408 + +4.1 3,442,416 - +1.4 3,442,416 - -0.9 3,442,457 - +0.3 3,442,526 + BWI76_RS16625 0.13 -2.5 3,442,526 + BWI76_RS16625 0.13 -0.0 3,442,592 + BWI76_RS16625 0.20 -0.9 3,442,729 + BWI76_RS16625 0.35 -0.8 3,442,776 - BWI76_RS16625 0.41 -2.3 3,443,007 + BWI76_RS16625 0.67 +0.2 3,443,062 + BWI76_RS16625 0.73 -0.7 3,443,215 + -0.1 3,443,399 + BWI76_RS16630 0.10 -0.2 3,443,415 + BWI76_RS16630 0.12 -0.0 3,443,588 + BWI76_RS16630 0.33 +0.6 3,443,596 - BWI76_RS16630 0.34 +1.6 3,443,596 - BWI76_RS16630 0.34 +0.0 3,443,672 - BWI76_RS16630 0.44 -0.4 3,443,779 - BWI76_RS16630 0.57 +0.3 3,443,952 + BWI76_RS16630 0.79 -0.0 3,444,163 - +0.1 3,444,163 - -1.3 3,444,163 - -0.5 3,444,184 + -0.4 3,444,202 - +1.3
Or see this region's nucleotide sequence