Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16445

Experiment: K. M5a1 KLEB10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16430 and BWI76_RS16435 are separated by 41 nucleotidesBWI76_RS16435 and BWI76_RS16440 are separated by 171 nucleotidesBWI76_RS16440 and BWI76_RS16445 are separated by 10 nucleotidesBWI76_RS16445 and BWI76_RS16450 overlap by 1 nucleotides BWI76_RS16430: BWI76_RS16430 - GGDEF domain-containing protein, at 3,408,369 to 3,409,721 _RS16430 BWI76_RS16435: BWI76_RS16435 - SlyA family transcriptional regulator, at 3,409,763 to 3,410,203 _RS16435 BWI76_RS16440: BWI76_RS16440 - DUF1656 domain-containing protein, at 3,410,375 to 3,410,611 _RS16440 BWI76_RS16445: BWI76_RS16445 - auxiliary transporter membrane fusion protein, at 3,410,622 to 3,411,518 _RS16445 BWI76_RS16450: BWI76_RS16450 - fusaric acid resistance protein, at 3,411,518 to 3,413,542 _RS16450 Position (kb) 3410 3411 3412Strain fitness (log2 ratio) -1 0 1 2at 3409.719 kb on - strandat 3410.839 kb on - strand, within BWI76_RS16445at 3411.160 kb on + strand, within BWI76_RS16445at 3411.200 kb on - strand, within BWI76_RS16445at 3411.515 kb on + strandat 3411.893 kb on + strand, within BWI76_RS16450at 3411.893 kb on + strand, within BWI76_RS16450at 3411.893 kb on + strand, within BWI76_RS16450at 3411.901 kb on - strand, within BWI76_RS16450at 3411.901 kb on - strand, within BWI76_RS16450at 3412.190 kb on + strand, within BWI76_RS16450at 3412.348 kb on - strand, within BWI76_RS16450

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1
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3,409,719 - +1.2
3,410,839 - BWI76_RS16445 0.24 +0.3
3,411,160 + BWI76_RS16445 0.60 +1.7
3,411,200 - BWI76_RS16445 0.64 -0.6
3,411,515 + +0.3
3,411,893 + BWI76_RS16450 0.19 -1.2
3,411,893 + BWI76_RS16450 0.19 +0.0
3,411,893 + BWI76_RS16450 0.19 +1.5
3,411,901 - BWI76_RS16450 0.19 -0.0
3,411,901 - BWI76_RS16450 0.19 +0.4
3,412,190 + BWI76_RS16450 0.33 +1.9
3,412,348 - BWI76_RS16450 0.41 -0.4

Or see this region's nucleotide sequence