Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16430 and BWI76_RS16435 are separated by 41 nucleotides BWI76_RS16435 and BWI76_RS16440 are separated by 171 nucleotides BWI76_RS16440 and BWI76_RS16445 are separated by 10 nucleotides BWI76_RS16445 and BWI76_RS16450 overlap by 1 nucleotides
BWI76_RS16430: BWI76_RS16430 - GGDEF domain-containing protein, at 3,408,369 to 3,409,721
_RS16430
BWI76_RS16435: BWI76_RS16435 - SlyA family transcriptional regulator, at 3,409,763 to 3,410,203
_RS16435
BWI76_RS16440: BWI76_RS16440 - DUF1656 domain-containing protein, at 3,410,375 to 3,410,611
_RS16440
BWI76_RS16445: BWI76_RS16445 - auxiliary transporter membrane fusion protein, at 3,410,622 to 3,411,518
_RS16445
BWI76_RS16450: BWI76_RS16450 - fusaric acid resistance protein, at 3,411,518 to 3,413,542
_RS16450
Position (kb) 3410
3411 Strain fitness (log2 ratio) -1
0
1 at 3409.387 kb on + strand, within BWI76_RS16430 at 3409.597 kb on + strand at 3409.605 kb on - strand at 3409.719 kb on - strand at 3410.839 kb on - strand, within BWI76_RS16445 at 3411.160 kb on + strand, within BWI76_RS16445 at 3411.200 kb on - strand, within BWI76_RS16445 at 3411.515 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 3,409,387 + BWI76_RS16430 0.75 -0.4 3,409,597 + -0.0 3,409,605 - +1.0 3,409,719 - +1.2 3,410,839 - BWI76_RS16445 0.24 +0.3 3,411,160 + BWI76_RS16445 0.60 +1.7 3,411,200 - BWI76_RS16445 0.64 -0.6 3,411,515 + +0.3
Or see this region's nucleotide sequence