Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15225 and BWI76_RS15230 are separated by 1 nucleotides BWI76_RS15230 and BWI76_RS15235 overlap by 8 nucleotides BWI76_RS15235 and BWI76_RS15240 are separated by 176 nucleotides
BWI76_RS15225: BWI76_RS15225 - DeoR family transcriptional regulator, at 3,191,051 to 3,191,812
_RS15225
BWI76_RS15230: BWI76_RS15230 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 3,191,814 to 3,192,800
_RS15230
BWI76_RS15235: BWI76_RS15235 - hypothetical protein, at 3,192,793 to 3,194,031
_RS15235
BWI76_RS15240: BWI76_RS15240 - alcohol dehydrogenase, at 3,194,208 to 3,195,353
_RS15240
Position (kb) 3192
3193
3194
3195 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3191.848 kb on - strand at 3191.851 kb on - strand at 3192.218 kb on + strand, within BWI76_RS15230 at 3192.243 kb on - strand, within BWI76_RS15230 at 3192.280 kb on + strand, within BWI76_RS15230 at 3192.316 kb on + strand, within BWI76_RS15230 at 3192.592 kb on + strand, within BWI76_RS15230 at 3192.728 kb on - strand at 3192.837 kb on - strand at 3193.545 kb on + strand, within BWI76_RS15235 at 3193.586 kb on + strand, within BWI76_RS15235 at 3193.808 kb on - strand, within BWI76_RS15235 at 3194.018 kb on - strand at 3194.366 kb on - strand, within BWI76_RS15240 at 3194.389 kb on - strand, within BWI76_RS15240 at 3194.419 kb on - strand, within BWI76_RS15240 at 3194.522 kb on - strand, within BWI76_RS15240 at 3194.658 kb on - strand, within BWI76_RS15240
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 3,191,848 - -0.5 3,191,851 - -0.6 3,192,218 + BWI76_RS15230 0.41 -0.5 3,192,243 - BWI76_RS15230 0.43 -0.6 3,192,280 + BWI76_RS15230 0.47 -1.9 3,192,316 + BWI76_RS15230 0.51 +2.1 3,192,592 + BWI76_RS15230 0.79 +0.6 3,192,728 - -0.8 3,192,837 - +0.2 3,193,545 + BWI76_RS15235 0.61 -2.7 3,193,586 + BWI76_RS15235 0.64 -0.6 3,193,808 - BWI76_RS15235 0.82 -2.1 3,194,018 - +0.3 3,194,366 - BWI76_RS15240 0.14 -1.2 3,194,389 - BWI76_RS15240 0.16 -1.9 3,194,419 - BWI76_RS15240 0.18 +0.5 3,194,522 - BWI76_RS15240 0.27 +0.4 3,194,658 - BWI76_RS15240 0.39 +0.3
Or see this region's nucleotide sequence