Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14220 and BWI76_RS14225 are separated by 53 nucleotides BWI76_RS14225 and BWI76_RS14230 overlap by 1 nucleotides BWI76_RS14230 and BWI76_RS14235 are separated by 84 nucleotides
BWI76_RS14220: BWI76_RS14220 - hypothetical protein, at 2,976,163 to 2,976,504
_RS14220
BWI76_RS14225: BWI76_RS14225 - anti-ECF sigma factor ChrR, at 2,976,558 to 2,977,229
_RS14225
BWI76_RS14230: BWI76_RS14230 - flavin monoamine oxidase family protein, at 2,977,229 to 2,978,218
_RS14230
BWI76_RS14235: BWI76_RS14235 - alcohol dehydrogenase, at 2,978,303 to 2,979,327
_RS14235
Position (kb)
2977
2978
2979 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2976.672 kb on + strand, within BWI76_RS14225 at 2976.680 kb on - strand, within BWI76_RS14225 at 2977.381 kb on + strand, within BWI76_RS14230 at 2977.774 kb on + strand, within BWI76_RS14230 at 2977.774 kb on + strand, within BWI76_RS14230 at 2977.818 kb on - strand, within BWI76_RS14230 at 2977.994 kb on + strand, within BWI76_RS14230 at 2978.403 kb on - strand at 2978.449 kb on - strand, within BWI76_RS14235 at 2978.546 kb on - strand, within BWI76_RS14235 at 2978.552 kb on - strand, within BWI76_RS14235 at 2978.552 kb on - strand, within BWI76_RS14235 at 2978.702 kb on - strand, within BWI76_RS14235 at 2978.744 kb on + strand, within BWI76_RS14235 at 2978.892 kb on + strand, within BWI76_RS14235 at 2978.900 kb on - strand, within BWI76_RS14235 at 2978.940 kb on + strand, within BWI76_RS14235
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 2,976,672 + BWI76_RS14225 0.17 -1.2 2,976,680 - BWI76_RS14225 0.18 -1.0 2,977,381 + BWI76_RS14230 0.15 +0.0 2,977,774 + BWI76_RS14230 0.55 -1.5 2,977,774 + BWI76_RS14230 0.55 -1.4 2,977,818 - BWI76_RS14230 0.59 -0.6 2,977,994 + BWI76_RS14230 0.77 -1.1 2,978,403 - -0.3 2,978,449 - BWI76_RS14235 0.14 -0.2 2,978,546 - BWI76_RS14235 0.24 -0.1 2,978,552 - BWI76_RS14235 0.24 -1.0 2,978,552 - BWI76_RS14235 0.24 -3.7 2,978,702 - BWI76_RS14235 0.39 +1.0 2,978,744 + BWI76_RS14235 0.43 -1.9 2,978,892 + BWI76_RS14235 0.57 -0.0 2,978,900 - BWI76_RS14235 0.58 -0.1 2,978,940 + BWI76_RS14235 0.62 -1.9
Or see this region's nucleotide sequence