Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12910 and BWI76_RS12915 are separated by 17 nucleotides BWI76_RS12915 and BWI76_RS12920 are separated by 51 nucleotides BWI76_RS12920 and BWI76_RS12925 overlap by 4 nucleotides
BWI76_RS12910: BWI76_RS12910 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 2,689,937 to 2,690,743
_RS12910
BWI76_RS12915: BWI76_RS12915 - 4-hydroxyphenylacetate permease, at 2,690,761 to 2,692,134
_RS12915
BWI76_RS12920: BWI76_RS12920 - DNA-binding protein, at 2,692,186 to 2,692,797
_RS12920
BWI76_RS12925: BWI76_RS12925 - efflux transporter periplasmic adaptor subunit, at 2,692,794 to 2,693,717
_RS12925
Position (kb)
2690
2691
2692
2693 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2689.870 kb on + strand at 2689.870 kb on + strand at 2689.870 kb on + strand at 2689.870 kb on + strand at 2689.870 kb on + strand at 2689.878 kb on - strand at 2689.878 kb on - strand at 2689.883 kb on + strand at 2689.911 kb on - strand at 2689.920 kb on + strand at 2689.920 kb on + strand at 2689.928 kb on - strand at 2690.001 kb on + strand at 2690.010 kb on + strand at 2690.017 kb on + strand at 2690.067 kb on + strand, within BWI76_RS12910 at 2690.149 kb on - strand, within BWI76_RS12910 at 2690.251 kb on - strand, within BWI76_RS12910 at 2690.780 kb on - strand at 2690.823 kb on + strand at 2690.978 kb on - strand, within BWI76_RS12915 at 2691.044 kb on - strand, within BWI76_RS12915 at 2691.073 kb on + strand, within BWI76_RS12915 at 2691.073 kb on + strand, within BWI76_RS12915 at 2691.369 kb on + strand, within BWI76_RS12915 at 2691.714 kb on + strand, within BWI76_RS12915 at 2691.751 kb on - strand, within BWI76_RS12915 at 2691.858 kb on + strand, within BWI76_RS12915 at 2691.901 kb on - strand, within BWI76_RS12915 at 2692.037 kb on - strand at 2692.460 kb on - strand, within BWI76_RS12920 at 2692.821 kb on + strand at 2692.824 kb on - strand at 2692.996 kb on - strand, within BWI76_RS12925
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 2,689,870 + -1.2 2,689,870 + +0.3 2,689,870 + -2.0 2,689,870 + -2.5 2,689,870 + -0.4 2,689,878 - -0.9 2,689,878 - -1.9 2,689,883 + +0.5 2,689,911 - +0.1 2,689,920 + +0.4 2,689,920 + -2.3 2,689,928 - +2.6 2,690,001 + +0.2 2,690,010 + -0.0 2,690,017 + +0.6 2,690,067 + BWI76_RS12910 0.16 +0.5 2,690,149 - BWI76_RS12910 0.26 +0.5 2,690,251 - BWI76_RS12910 0.39 +1.2 2,690,780 - +0.4 2,690,823 + +0.3 2,690,978 - BWI76_RS12915 0.16 -2.0 2,691,044 - BWI76_RS12915 0.21 -2.7 2,691,073 + BWI76_RS12915 0.23 -2.5 2,691,073 + BWI76_RS12915 0.23 -0.4 2,691,369 + BWI76_RS12915 0.44 -1.3 2,691,714 + BWI76_RS12915 0.69 +0.1 2,691,751 - BWI76_RS12915 0.72 -1.8 2,691,858 + BWI76_RS12915 0.80 -0.8 2,691,901 - BWI76_RS12915 0.83 -0.7 2,692,037 - -0.2 2,692,460 - BWI76_RS12920 0.45 -2.8 2,692,821 + -2.6 2,692,824 - +0.9 2,692,996 - BWI76_RS12925 0.22 -0.1
Or see this region's nucleotide sequence