Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12915

Experiment: K. M5a1 KLEB10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12910 and BWI76_RS12915 are separated by 17 nucleotidesBWI76_RS12915 and BWI76_RS12920 are separated by 51 nucleotidesBWI76_RS12920 and BWI76_RS12925 overlap by 4 nucleotides BWI76_RS12910: BWI76_RS12910 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 2,689,937 to 2,690,743 _RS12910 BWI76_RS12915: BWI76_RS12915 - 4-hydroxyphenylacetate permease, at 2,690,761 to 2,692,134 _RS12915 BWI76_RS12920: BWI76_RS12920 - DNA-binding protein, at 2,692,186 to 2,692,797 _RS12920 BWI76_RS12925: BWI76_RS12925 - efflux transporter periplasmic adaptor subunit, at 2,692,794 to 2,693,717 _RS12925 Position (kb) 2690 2691 2692 2693Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2689.870 kb on + strandat 2689.870 kb on + strandat 2689.870 kb on + strandat 2689.870 kb on + strandat 2689.870 kb on + strandat 2689.878 kb on - strandat 2689.878 kb on - strandat 2689.883 kb on + strandat 2689.911 kb on - strandat 2689.920 kb on + strandat 2689.920 kb on + strandat 2689.928 kb on - strandat 2690.001 kb on + strandat 2690.010 kb on + strandat 2690.017 kb on + strandat 2690.067 kb on + strand, within BWI76_RS12910at 2690.149 kb on - strand, within BWI76_RS12910at 2690.251 kb on - strand, within BWI76_RS12910at 2690.780 kb on - strandat 2690.823 kb on + strandat 2690.978 kb on - strand, within BWI76_RS12915at 2691.044 kb on - strand, within BWI76_RS12915at 2691.073 kb on + strand, within BWI76_RS12915at 2691.073 kb on + strand, within BWI76_RS12915at 2691.369 kb on + strand, within BWI76_RS12915at 2691.714 kb on + strand, within BWI76_RS12915at 2691.751 kb on - strand, within BWI76_RS12915at 2691.858 kb on + strand, within BWI76_RS12915at 2691.901 kb on - strand, within BWI76_RS12915at 2692.037 kb on - strandat 2692.460 kb on - strand, within BWI76_RS12920at 2692.821 kb on + strandat 2692.824 kb on - strandat 2692.996 kb on - strand, within BWI76_RS12925

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1
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2,689,870 + -1.2
2,689,870 + +0.3
2,689,870 + -2.0
2,689,870 + -2.5
2,689,870 + -0.4
2,689,878 - -0.9
2,689,878 - -1.9
2,689,883 + +0.5
2,689,911 - +0.1
2,689,920 + +0.4
2,689,920 + -2.3
2,689,928 - +2.6
2,690,001 + +0.2
2,690,010 + -0.0
2,690,017 + +0.6
2,690,067 + BWI76_RS12910 0.16 +0.5
2,690,149 - BWI76_RS12910 0.26 +0.5
2,690,251 - BWI76_RS12910 0.39 +1.2
2,690,780 - +0.4
2,690,823 + +0.3
2,690,978 - BWI76_RS12915 0.16 -2.0
2,691,044 - BWI76_RS12915 0.21 -2.7
2,691,073 + BWI76_RS12915 0.23 -2.5
2,691,073 + BWI76_RS12915 0.23 -0.4
2,691,369 + BWI76_RS12915 0.44 -1.3
2,691,714 + BWI76_RS12915 0.69 +0.1
2,691,751 - BWI76_RS12915 0.72 -1.8
2,691,858 + BWI76_RS12915 0.80 -0.8
2,691,901 - BWI76_RS12915 0.83 -0.7
2,692,037 - -0.2
2,692,460 - BWI76_RS12920 0.45 -2.8
2,692,821 + -2.6
2,692,824 - +0.9
2,692,996 - BWI76_RS12925 0.22 -0.1

Or see this region's nucleotide sequence