Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11220 and BWI76_RS11225 are separated by 48 nucleotides BWI76_RS11225 and BWI76_RS11230 are separated by 14 nucleotides BWI76_RS11230 and BWI76_RS11235 are separated by 28 nucleotides BWI76_RS11235 and BWI76_RS11240 are separated by 346 nucleotides
BWI76_RS11220: BWI76_RS11220 - lipoprotein transporter subunit LolE, at 2,367,462 to 2,368,706
_RS11220
BWI76_RS11225: BWI76_RS11225 - N-acetylglucosamine kinase, at 2,368,755 to 2,369,666
_RS11225
BWI76_RS11230: BWI76_RS11230 - NAD-dependent deacylase, at 2,369,681 to 2,370,526
_RS11230
BWI76_RS11235: BWI76_RS11235 - hypothetical protein, at 2,370,555 to 2,370,860
_RS11235
BWI76_RS11240: BWI76_RS11240 - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 2,371,207 to 2,372,253
_RS11240
Position (kb)
2369
2370
2371 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2368.804 kb on - strand at 2369.610 kb on + strand at 2369.628 kb on + strand at 2369.636 kb on - strand at 2369.735 kb on + strand at 2369.988 kb on - strand, within BWI76_RS11230 at 2370.211 kb on - strand, within BWI76_RS11230 at 2370.335 kb on - strand, within BWI76_RS11230 at 2370.442 kb on + strand at 2370.552 kb on + strand at 2370.560 kb on - strand at 2370.623 kb on + strand, within BWI76_RS11235 at 2370.904 kb on + strand at 2371.030 kb on + strand at 2371.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 2,368,804 - -0.2 2,369,610 + -0.2 2,369,628 + -1.7 2,369,636 - +0.9 2,369,735 + -0.4 2,369,988 - BWI76_RS11230 0.36 +2.0 2,370,211 - BWI76_RS11230 0.63 +0.8 2,370,335 - BWI76_RS11230 0.77 -2.5 2,370,442 + -0.1 2,370,552 + +0.2 2,370,560 - -1.2 2,370,623 + BWI76_RS11235 0.22 -3.3 2,370,904 + +1.2 2,371,030 + -0.6 2,371,166 - +0.8
Or see this region's nucleotide sequence