Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06795 and BWI76_RS06800 are separated by 66 nucleotides BWI76_RS06800 and BWI76_RS06805 are separated by 14 nucleotides BWI76_RS06805 and BWI76_RS06810 are separated by 69 nucleotides BWI76_RS06810 and BWI76_RS06815 are separated by 150 nucleotides BWI76_RS06815 and BWI76_RS06820 are separated by 91 nucleotides
BWI76_RS06795: BWI76_RS06795 - hypothetical protein, at 1,427,507 to 1,428,067
_RS06795
BWI76_RS06800: BWI76_RS06800 - DUF2496 domain-containing protein, at 1,428,134 to 1,428,307
_RS06800
BWI76_RS06805: BWI76_RS06805 - primosomal replication protein N'', at 1,428,322 to 1,428,849
_RS06805
BWI76_RS06810: BWI76_RS06810 - hypothetical protein, at 1,428,919 to 1,429,296
_RS06810
BWI76_RS06815: BWI76_RS06815 - adenine phosphoribosyltransferase, at 1,429,447 to 1,429,998
_RS06815
BWI76_RS06820: BWI76_RS06820 - DNA polymerase III subunit gamma/tau, at 1,430,090 to 1,431,991
_RS06820
Position (kb)
1428
1429
1430 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1428.046 kb on + strand at 1428.274 kb on + strand, within BWI76_RS06800 at 1428.450 kb on - strand, within BWI76_RS06805 at 1428.556 kb on - strand, within BWI76_RS06805 at 1428.707 kb on + strand, within BWI76_RS06805 at 1428.879 kb on + strand at 1428.974 kb on - strand, within BWI76_RS06810 at 1429.029 kb on + strand, within BWI76_RS06810 at 1429.141 kb on - strand, within BWI76_RS06810 at 1429.250 kb on - strand, within BWI76_RS06810 at 1429.290 kb on + strand at 1429.367 kb on - strand at 1429.498 kb on + strand at 1429.498 kb on + strand at 1429.506 kb on - strand, within BWI76_RS06815 at 1429.585 kb on - strand, within BWI76_RS06815 at 1429.696 kb on - strand, within BWI76_RS06815 at 1429.891 kb on + strand, within BWI76_RS06815
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 1,428,046 + +0.5 1,428,274 + BWI76_RS06800 0.80 +0.2 1,428,450 - BWI76_RS06805 0.24 +0.2 1,428,556 - BWI76_RS06805 0.44 +1.4 1,428,707 + BWI76_RS06805 0.73 -0.1 1,428,879 + -0.7 1,428,974 - BWI76_RS06810 0.15 -3.1 1,429,029 + BWI76_RS06810 0.29 -1.8 1,429,141 - BWI76_RS06810 0.59 +0.8 1,429,250 - BWI76_RS06810 0.88 -0.2 1,429,290 + +1.6 1,429,367 - -2.9 1,429,498 + +1.8 1,429,498 + -2.2 1,429,506 - BWI76_RS06815 0.11 -1.5 1,429,585 - BWI76_RS06815 0.25 -0.1 1,429,696 - BWI76_RS06815 0.45 +0.4 1,429,891 + BWI76_RS06815 0.80 +0.1
Or see this region's nucleotide sequence