Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06315 and BWI76_RS06320 are separated by 115 nucleotides BWI76_RS06320 and BWI76_RS06325 are separated by 64 nucleotides BWI76_RS06325 and BWI76_RS06330 are separated by 148 nucleotides
BWI76_RS06315: BWI76_RS06315 - protein translocase subunit SecF, at 1,329,504 to 1,330,475
_RS06315
BWI76_RS06320: BWI76_RS06320 - Xaa-Pro aminopeptidase, at 1,330,591 to 1,331,982
_RS06320
BWI76_RS06325: BWI76_RS06325 - hypothetical protein, at 1,332,047 to 1,332,424
_RS06325
BWI76_RS06330: BWI76_RS06330 - DNA-binding transcriptional regulator, at 1,332,573 to 1,333,262
_RS06330
Position (kb)
1330
1331
1332 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1330.726 kb on - strand at 1331.238 kb on + strand, within BWI76_RS06320 at 1331.246 kb on + strand, within BWI76_RS06320 at 1331.734 kb on + strand, within BWI76_RS06320 at 1331.830 kb on - strand, within BWI76_RS06320 at 1331.994 kb on + strand at 1332.002 kb on - strand at 1332.004 kb on + strand at 1332.004 kb on + strand at 1332.012 kb on - strand at 1332.012 kb on - strand at 1332.064 kb on - strand at 1332.064 kb on - strand at 1332.083 kb on + strand at 1332.223 kb on + strand, within BWI76_RS06325 at 1332.231 kb on + strand, within BWI76_RS06325 at 1332.231 kb on - strand, within BWI76_RS06325 at 1332.762 kb on + strand, within BWI76_RS06330 at 1332.953 kb on + strand, within BWI76_RS06330
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 1,330,726 - +0.5 1,331,238 + BWI76_RS06320 0.46 +0.5 1,331,246 + BWI76_RS06320 0.47 +1.3 1,331,734 + BWI76_RS06320 0.82 +0.8 1,331,830 - BWI76_RS06320 0.89 +0.4 1,331,994 + -0.5 1,332,002 - +0.2 1,332,004 + -0.4 1,332,004 + +0.3 1,332,012 - +0.7 1,332,012 - -0.2 1,332,064 - -1.0 1,332,064 - -1.1 1,332,083 + -2.8 1,332,223 + BWI76_RS06325 0.47 -0.3 1,332,231 + BWI76_RS06325 0.49 +1.3 1,332,231 - BWI76_RS06325 0.49 +0.6 1,332,762 + BWI76_RS06330 0.27 +0.9 1,332,953 + BWI76_RS06330 0.55 +0.7
Or see this region's nucleotide sequence