Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04430 and BWI76_RS04435 are separated by 278 nucleotides BWI76_RS04435 and BWI76_RS04440 are separated by 31 nucleotides BWI76_RS04440 and BWI76_RS04445 overlap by 4 nucleotides
BWI76_RS04430: BWI76_RS04430 - citrate carrier protein, at 914,716 to 916,056
_RS04430
BWI76_RS04435: BWI76_RS04435 - [citrate (pro-3S)-lyase] ligase, at 916,335 to 917,363
_RS04435
BWI76_RS04440: BWI76_RS04440 - citrate lyase ACP, at 917,395 to 917,688
_RS04440
BWI76_RS04445: BWI76_RS04445 - citrate (pro-3S)-lyase subunit beta, at 917,685 to 918,554
_RS04445
Position (kb) 916
917
918 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 915.342 kb on - strand, within BWI76_RS04430 at 915.389 kb on + strand, within BWI76_RS04430 at 915.397 kb on - strand, within BWI76_RS04430 at 915.400 kb on + strand, within BWI76_RS04430 at 915.406 kb on + strand, within BWI76_RS04430 at 915.411 kb on + strand at 915.423 kb on + strand, within BWI76_RS04430 at 915.423 kb on + strand, within BWI76_RS04430 at 915.425 kb on + strand, within BWI76_RS04430 at 915.430 kb on + strand, within BWI76_RS04430 at 915.431 kb on - strand, within BWI76_RS04430 at 915.431 kb on - strand, within BWI76_RS04430 at 915.438 kb on - strand, within BWI76_RS04430 at 915.478 kb on + strand, within BWI76_RS04430 at 915.497 kb on - strand, within BWI76_RS04430 at 915.497 kb on - strand, within BWI76_RS04430 at 915.531 kb on - strand, within BWI76_RS04430 at 915.532 kb on - strand, within BWI76_RS04430 at 915.532 kb on - strand, within BWI76_RS04430 at 915.534 kb on - strand, within BWI76_RS04430 at 915.557 kb on - strand, within BWI76_RS04430 at 915.571 kb on + strand, within BWI76_RS04430 at 915.576 kb on - strand, within BWI76_RS04430 at 915.579 kb on - strand, within BWI76_RS04430 at 915.589 kb on - strand, within BWI76_RS04430 at 915.601 kb on - strand, within BWI76_RS04430 at 915.601 kb on - strand, within BWI76_RS04430 at 915.621 kb on + strand, within BWI76_RS04430 at 915.629 kb on - strand, within BWI76_RS04430 at 915.629 kb on - strand, within BWI76_RS04430 at 915.629 kb on - strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.658 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.680 kb on + strand, within BWI76_RS04430 at 915.698 kb on + strand, within BWI76_RS04430 at 915.741 kb on + strand, within BWI76_RS04430 at 915.741 kb on + strand, within BWI76_RS04430 at 915.756 kb on + strand, within BWI76_RS04430 at 915.799 kb on + strand, within BWI76_RS04430 at 915.818 kb on + strand, within BWI76_RS04430 at 915.892 kb on - strand, within BWI76_RS04430 at 915.895 kb on + strand, within BWI76_RS04430 at 915.915 kb on + strand, within BWI76_RS04430 at 915.923 kb on - strand at 915.954 kb on + strand at 915.954 kb on + strand at 915.957 kb on - strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.962 kb on - strand at 915.969 kb on - strand at 915.969 kb on - strand at 915.969 kb on - strand at 915.970 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.044 kb on - strand at 916.068 kb on - strand at 916.238 kb on + strand at 916.294 kb on + strand at 916.294 kb on + strand at 916.303 kb on - strand at 916.389 kb on + strand at 916.390 kb on + strand at 916.390 kb on - strand at 916.397 kb on + strand at 916.398 kb on - strand at 916.418 kb on + strand at 916.418 kb on + strand at 916.426 kb on - strand at 916.438 kb on + strand, within BWI76_RS04435 at 916.461 kb on + strand, within BWI76_RS04435 at 916.503 kb on + strand, within BWI76_RS04435 at 916.503 kb on + strand, within BWI76_RS04435 at 916.526 kb on - strand, within BWI76_RS04435 at 916.552 kb on + strand, within BWI76_RS04435 at 916.552 kb on + strand, within BWI76_RS04435 at 916.560 kb on - strand, within BWI76_RS04435 at 916.560 kb on - strand, within BWI76_RS04435 at 916.565 kb on + strand, within BWI76_RS04435 at 916.571 kb on + strand, within BWI76_RS04435 at 916.585 kb on + strand, within BWI76_RS04435 at 916.830 kb on - strand, within BWI76_RS04435 at 917.148 kb on - strand, within BWI76_RS04435 at 917.237 kb on + strand, within BWI76_RS04435 at 917.418 kb on + strand at 917.461 kb on - strand, within BWI76_RS04440 at 917.559 kb on + strand, within BWI76_RS04440 at 917.615 kb on - strand, within BWI76_RS04440 at 917.653 kb on + strand, within BWI76_RS04440 at 917.759 kb on - strand at 917.935 kb on + strand, within BWI76_RS04445 at 918.031 kb on + strand, within BWI76_RS04445 at 918.031 kb on + strand, within BWI76_RS04445 at 918.046 kb on + strand, within BWI76_RS04445
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 915,342 - BWI76_RS04430 0.47 +0.6 915,389 + BWI76_RS04430 0.50 +1.5 915,397 - BWI76_RS04430 0.51 +0.9 915,400 + BWI76_RS04430 0.51 +0.3 915,406 + BWI76_RS04430 0.51 -0.3 915,411 + -1.6 915,423 + BWI76_RS04430 0.53 -0.6 915,423 + BWI76_RS04430 0.53 +0.5 915,425 + BWI76_RS04430 0.53 -0.4 915,430 + BWI76_RS04430 0.53 +1.0 915,431 - BWI76_RS04430 0.53 +1.6 915,431 - BWI76_RS04430 0.53 -1.7 915,438 - BWI76_RS04430 0.54 -0.4 915,478 + BWI76_RS04430 0.57 +1.2 915,497 - BWI76_RS04430 0.58 +0.2 915,497 - BWI76_RS04430 0.58 -2.9 915,531 - BWI76_RS04430 0.61 +0.1 915,532 - BWI76_RS04430 0.61 -2.3 915,532 - BWI76_RS04430 0.61 +0.4 915,534 - BWI76_RS04430 0.61 -2.1 915,557 - BWI76_RS04430 0.63 +0.4 915,571 + BWI76_RS04430 0.64 +1.8 915,576 - BWI76_RS04430 0.64 +0.1 915,579 - BWI76_RS04430 0.64 +1.4 915,589 - BWI76_RS04430 0.65 +0.5 915,601 - BWI76_RS04430 0.66 +0.3 915,601 - BWI76_RS04430 0.66 -1.4 915,621 + BWI76_RS04430 0.67 +2.0 915,629 - BWI76_RS04430 0.68 -1.1 915,629 - BWI76_RS04430 0.68 -1.7 915,629 - BWI76_RS04430 0.68 +0.7 915,651 + BWI76_RS04430 0.70 +0.2 915,651 + BWI76_RS04430 0.70 -0.8 915,651 + BWI76_RS04430 0.70 +0.5 915,651 + BWI76_RS04430 0.70 +0.7 915,651 + BWI76_RS04430 0.70 +0.1 915,651 + BWI76_RS04430 0.70 +0.6 915,651 + BWI76_RS04430 0.70 +2.3 915,651 + BWI76_RS04430 0.70 -0.8 915,651 + BWI76_RS04430 0.70 -2.6 915,651 + BWI76_RS04430 0.70 -2.4 915,651 + BWI76_RS04430 0.70 +0.1 915,651 + BWI76_RS04430 0.70 +0.1 915,651 + BWI76_RS04430 0.70 -1.9 915,658 - BWI76_RS04430 0.70 +0.7 915,659 - BWI76_RS04430 0.70 -2.2 915,659 - BWI76_RS04430 0.70 +4.2 915,659 - BWI76_RS04430 0.70 +1.3 915,659 - BWI76_RS04430 0.70 -0.9 915,659 - BWI76_RS04430 0.70 +1.9 915,680 + BWI76_RS04430 0.72 -0.6 915,698 + BWI76_RS04430 0.73 -1.4 915,741 + BWI76_RS04430 0.76 -0.8 915,741 + BWI76_RS04430 0.76 -0.5 915,756 + BWI76_RS04430 0.78 -1.0 915,799 + BWI76_RS04430 0.81 -0.7 915,818 + BWI76_RS04430 0.82 +0.7 915,892 - BWI76_RS04430 0.88 +1.3 915,895 + BWI76_RS04430 0.88 -1.3 915,915 + BWI76_RS04430 0.89 -0.2 915,923 - +1.4 915,954 + +0.4 915,954 + +0.3 915,957 - +0.3 915,961 + -0.8 915,961 + +1.2 915,961 + +2.3 915,961 + +1.5 915,961 + +0.1 915,962 - +0.6 915,969 - +4.2 915,969 - +1.4 915,969 - -1.4 915,970 - +1.0 916,039 - -0.0 916,039 - -1.1 916,039 - -0.3 916,039 - -1.9 916,039 - +0.5 916,039 - +0.0 916,044 - +0.8 916,068 - -0.4 916,238 + +0.8 916,294 + +2.7 916,294 + -2.2 916,303 - -0.4 916,389 + -0.7 916,390 + -0.3 916,390 - -0.4 916,397 + +0.6 916,398 - -2.6 916,418 + +0.3 916,418 + +1.4 916,426 - +0.7 916,438 + BWI76_RS04435 0.10 +0.5 916,461 + BWI76_RS04435 0.12 -0.6 916,503 + BWI76_RS04435 0.16 -1.1 916,503 + BWI76_RS04435 0.16 +0.2 916,526 - BWI76_RS04435 0.19 -0.9 916,552 + BWI76_RS04435 0.21 -0.8 916,552 + BWI76_RS04435 0.21 -1.0 916,560 - BWI76_RS04435 0.22 -0.3 916,560 - BWI76_RS04435 0.22 +0.1 916,565 + BWI76_RS04435 0.22 +1.6 916,571 + BWI76_RS04435 0.23 -0.3 916,585 + BWI76_RS04435 0.24 +0.1 916,830 - BWI76_RS04435 0.48 -0.1 917,148 - BWI76_RS04435 0.79 -1.4 917,237 + BWI76_RS04435 0.88 -0.3 917,418 + -0.7 917,461 - BWI76_RS04440 0.22 +0.1 917,559 + BWI76_RS04440 0.56 -0.6 917,615 - BWI76_RS04440 0.75 -0.7 917,653 + BWI76_RS04440 0.88 -0.0 917,759 - -0.5 917,935 + BWI76_RS04445 0.29 -0.3 918,031 + BWI76_RS04445 0.40 -0.4 918,031 + BWI76_RS04445 0.40 -0.2 918,046 + BWI76_RS04445 0.41 -0.1
Or see this region's nucleotide sequence