Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02980 and BWI76_RS02985 are separated by 67 nucleotides BWI76_RS02985 and BWI76_RS02990 are separated by 112 nucleotides BWI76_RS02990 and BWI76_RS02995 are separated by 12 nucleotides BWI76_RS02995 and BWI76_RS03000 are separated by 36 nucleotides
BWI76_RS02980: BWI76_RS02980 - type I magnesium transporting ATPase, at 610,237 to 612,945
_RS02980
BWI76_RS02985: BWI76_RS02985 - reactive intermediate/imine deaminase, at 613,013 to 613,399
_RS02985
BWI76_RS02990: BWI76_RS02990 - aspartate carbamoyltransferase regulatory subunit, at 613,512 to 613,973
_RS02990
BWI76_RS02995: BWI76_RS02995 - aspartate carbamoyltransferase, at 613,986 to 614,921
_RS02995
BWI76_RS03000: BWI76_RS03000 - PyrBI operon leader peptide, at 614,958 to 615,062
_RS03000
Position (kb) 613
614 Strain fitness (log2 ratio) -2
-1
0
1
2 at 613.314 kb on - strand, within BWI76_RS02985 at 613.348 kb on + strand, within BWI76_RS02985 at 613.349 kb on - strand, within BWI76_RS02985 at 613.552 kb on - strand at 613.889 kb on + strand, within BWI76_RS02990 at 613.889 kb on + strand, within BWI76_RS02990 at 613.889 kb on + strand, within BWI76_RS02990 at 613.917 kb on + strand, within BWI76_RS02990 at 613.941 kb on - strand at 614.067 kb on + strand at 614.248 kb on - strand, within BWI76_RS02995 at 614.303 kb on - strand, within BWI76_RS02995 at 614.318 kb on + strand, within BWI76_RS02995 at 614.407 kb on - strand, within BWI76_RS02995 at 614.469 kb on + strand, within BWI76_RS02995 at 614.469 kb on + strand, within BWI76_RS02995 at 614.477 kb on - strand, within BWI76_RS02995 at 614.488 kb on - strand, within BWI76_RS02995 at 614.503 kb on - strand, within BWI76_RS02995 at 614.557 kb on - strand, within BWI76_RS02995 at 614.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 613,314 - BWI76_RS02985 0.78 -0.2 613,348 + BWI76_RS02985 0.87 +0.2 613,349 - BWI76_RS02985 0.87 -0.2 613,552 - -1.7 613,889 + BWI76_RS02990 0.82 -0.1 613,889 + BWI76_RS02990 0.82 -2.6 613,889 + BWI76_RS02990 0.82 -2.5 613,917 + BWI76_RS02990 0.88 -0.3 613,941 - +0.1 614,067 + +0.5 614,248 - BWI76_RS02995 0.28 -0.2 614,303 - BWI76_RS02995 0.34 +0.7 614,318 + BWI76_RS02995 0.35 -1.7 614,407 - BWI76_RS02995 0.45 -0.4 614,469 + BWI76_RS02995 0.52 +0.7 614,469 + BWI76_RS02995 0.52 +2.3 614,477 - BWI76_RS02995 0.52 +0.2 614,488 - BWI76_RS02995 0.54 -0.3 614,503 - BWI76_RS02995 0.55 -0.6 614,557 - BWI76_RS02995 0.61 +0.8 614,919 - +0.7
Or see this region's nucleotide sequence