Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02820 and BWI76_RS02825 are separated by 64 nucleotides BWI76_RS02825 and BWI76_RS02830 are separated by 162 nucleotides BWI76_RS02830 and BWI76_RS02835 are separated by 327 nucleotides
BWI76_RS02820: BWI76_RS02820 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 576,022 to 577,395
_RS02820
BWI76_RS02825: BWI76_RS02825 - putative acyltransferase domain protein, at 577,460 to 578,005
_RS02825
BWI76_RS02830: BWI76_RS02830 - DUF4432 domain-containing protein, at 578,168 to 579,376
_RS02830
BWI76_RS02835: BWI76_RS02835 - iron-containing alcohol dehydrogenase, at 579,704 to 580,846
_RS02835
Position (kb) 578
579
580 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 577.269 kb on + strand at 577.396 kb on + strand at 577.522 kb on + strand, within BWI76_RS02825 at 577.528 kb on - strand, within BWI76_RS02825 at 577.552 kb on + strand, within BWI76_RS02825 at 577.560 kb on - strand, within BWI76_RS02825 at 577.634 kb on + strand, within BWI76_RS02825 at 577.911 kb on + strand, within BWI76_RS02825 at 578.213 kb on + strand at 578.264 kb on + strand at 578.272 kb on + strand at 578.272 kb on - strand at 578.277 kb on - strand at 578.282 kb on - strand at 578.307 kb on + strand, within BWI76_RS02830 at 578.358 kb on - strand, within BWI76_RS02830 at 578.425 kb on + strand, within BWI76_RS02830 at 578.623 kb on + strand, within BWI76_RS02830 at 578.732 kb on + strand, within BWI76_RS02830 at 578.920 kb on + strand, within BWI76_RS02830 at 578.920 kb on + strand, within BWI76_RS02830 at 578.921 kb on + strand, within BWI76_RS02830 at 578.922 kb on + strand, within BWI76_RS02830 at 578.930 kb on - strand, within BWI76_RS02830 at 578.930 kb on - strand, within BWI76_RS02830 at 578.930 kb on - strand, within BWI76_RS02830 at 578.930 kb on - strand, within BWI76_RS02830 at 579.006 kb on - strand, within BWI76_RS02830 at 579.079 kb on - strand, within BWI76_RS02830 at 579.110 kb on - strand, within BWI76_RS02830 at 579.120 kb on - strand, within BWI76_RS02830 at 579.136 kb on + strand, within BWI76_RS02830 at 579.144 kb on - strand, within BWI76_RS02830 at 579.144 kb on - strand, within BWI76_RS02830 at 579.144 kb on - strand, within BWI76_RS02830 at 579.144 kb on - strand, within BWI76_RS02830 at 579.144 kb on - strand, within BWI76_RS02830 at 579.144 kb on - strand, within BWI76_RS02830 at 579.144 kb on - strand, within BWI76_RS02830 at 579.153 kb on + strand, within BWI76_RS02830 at 579.156 kb on - strand, within BWI76_RS02830 at 579.169 kb on - strand, within BWI76_RS02830 at 579.172 kb on + strand, within BWI76_RS02830 at 579.240 kb on + strand, within BWI76_RS02830 at 579.302 kb on - strand at 579.340 kb on - strand at 579.354 kb on - strand at 579.398 kb on + strand at 579.453 kb on + strand at 579.453 kb on + strand at 579.453 kb on + strand at 579.453 kb on + strand at 579.461 kb on - strand at 579.462 kb on - strand at 579.462 kb on - strand at 579.463 kb on - strand at 579.576 kb on + strand at 579.585 kb on + strand at 579.585 kb on + strand at 579.586 kb on + strand at 579.591 kb on + strand at 579.593 kb on - strand at 579.598 kb on + strand at 579.598 kb on + strand at 579.601 kb on + strand at 579.601 kb on + strand at 579.603 kb on + strand at 579.604 kb on - strand at 579.609 kb on - strand at 579.619 kb on - strand at 579.619 kb on - strand at 579.619 kb on - strand at 579.627 kb on - strand at 579.628 kb on - strand at 579.634 kb on + strand at 579.653 kb on + strand at 579.666 kb on + strand at 579.666 kb on - strand at 579.668 kb on + strand at 579.668 kb on + strand at 579.668 kb on + strand at 579.676 kb on - strand at 579.676 kb on - strand at 579.676 kb on - strand at 579.676 kb on - strand at 579.676 kb on - strand at 579.719 kb on - strand at 579.725 kb on + strand at 579.726 kb on + strand at 579.726 kb on + strand at 579.729 kb on - strand at 579.729 kb on - strand at 579.734 kb on - strand at 579.734 kb on - strand at 579.765 kb on + strand at 579.767 kb on - strand at 579.769 kb on + strand at 579.769 kb on + strand at 579.770 kb on + strand at 579.777 kb on + strand at 579.778 kb on - strand at 579.779 kb on - strand at 579.784 kb on - strand at 579.784 kb on - strand at 579.812 kb on + strand at 579.842 kb on + strand, within BWI76_RS02835 at 579.843 kb on - strand, within BWI76_RS02835 at 579.862 kb on + strand, within BWI76_RS02835 at 579.862 kb on + strand, within BWI76_RS02835 at 579.862 kb on + strand, within BWI76_RS02835 at 579.862 kb on + strand, within BWI76_RS02835 at 579.870 kb on - strand, within BWI76_RS02835 at 579.870 kb on - strand, within BWI76_RS02835 at 579.870 kb on - strand, within BWI76_RS02835 at 579.870 kb on - strand, within BWI76_RS02835 at 579.870 kb on - strand, within BWI76_RS02835 at 579.888 kb on - strand, within BWI76_RS02835 at 579.892 kb on + strand, within BWI76_RS02835 at 579.912 kb on + strand, within BWI76_RS02835 at 579.919 kb on + strand, within BWI76_RS02835 at 579.927 kb on - strand, within BWI76_RS02835 at 579.939 kb on + strand, within BWI76_RS02835 at 579.995 kb on - strand, within BWI76_RS02835 at 580.015 kb on + strand, within BWI76_RS02835 at 580.015 kb on + strand, within BWI76_RS02835 at 580.015 kb on + strand, within BWI76_RS02835 at 580.021 kb on + strand, within BWI76_RS02835 at 580.044 kb on + strand, within BWI76_RS02835 at 580.063 kb on + strand, within BWI76_RS02835 at 580.064 kb on + strand, within BWI76_RS02835 at 580.076 kb on - strand, within BWI76_RS02835 at 580.080 kb on + strand, within BWI76_RS02835 at 580.080 kb on + strand, within BWI76_RS02835 at 580.080 kb on + strand, within BWI76_RS02835 at 580.080 kb on + strand, within BWI76_RS02835 at 580.088 kb on - strand, within BWI76_RS02835 at 580.095 kb on + strand, within BWI76_RS02835 at 580.105 kb on + strand, within BWI76_RS02835 at 580.105 kb on + strand, within BWI76_RS02835 at 580.105 kb on + strand, within BWI76_RS02835 at 580.105 kb on + strand, within BWI76_RS02835 at 580.113 kb on - strand, within BWI76_RS02835 at 580.113 kb on - strand, within BWI76_RS02835 at 580.118 kb on + strand, within BWI76_RS02835 at 580.118 kb on + strand, within BWI76_RS02835 at 580.118 kb on + strand, within BWI76_RS02835 at 580.118 kb on + strand, within BWI76_RS02835 at 580.126 kb on - strand, within BWI76_RS02835 at 580.126 kb on - strand, within BWI76_RS02835 at 580.126 kb on - strand, within BWI76_RS02835 at 580.126 kb on - strand, within BWI76_RS02835 at 580.144 kb on + strand, within BWI76_RS02835 at 580.149 kb on + strand, within BWI76_RS02835 at 580.175 kb on + strand, within BWI76_RS02835 at 580.183 kb on - strand, within BWI76_RS02835 at 580.186 kb on + strand, within BWI76_RS02835 at 580.220 kb on + strand, within BWI76_RS02835 at 580.252 kb on - strand, within BWI76_RS02835 at 580.252 kb on - strand, within BWI76_RS02835 at 580.252 kb on - strand, within BWI76_RS02835 at 580.252 kb on - strand, within BWI76_RS02835 at 580.252 kb on - strand, within BWI76_RS02835 at 580.254 kb on + strand, within BWI76_RS02835 at 580.262 kb on - strand, within BWI76_RS02835 at 580.267 kb on + strand, within BWI76_RS02835 at 580.268 kb on + strand, within BWI76_RS02835 at 580.268 kb on + strand, within BWI76_RS02835 at 580.268 kb on + strand, within BWI76_RS02835 at 580.275 kb on - strand, within BWI76_RS02835 at 580.277 kb on + strand, within BWI76_RS02835 at 580.277 kb on + strand, within BWI76_RS02835 at 580.359 kb on + strand, within BWI76_RS02835
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 577,269 + +0.1 577,396 + +0.1 577,522 + BWI76_RS02825 0.11 -0.0 577,528 - BWI76_RS02825 0.12 +0.1 577,552 + BWI76_RS02825 0.17 +0.4 577,560 - BWI76_RS02825 0.18 +0.1 577,634 + BWI76_RS02825 0.32 -1.0 577,911 + BWI76_RS02825 0.83 -0.4 578,213 + -0.5 578,264 + -0.2 578,272 + -1.0 578,272 - +0.3 578,277 - -1.5 578,282 - +0.4 578,307 + BWI76_RS02830 0.11 -0.2 578,358 - BWI76_RS02830 0.16 -0.6 578,425 + BWI76_RS02830 0.21 -0.6 578,623 + BWI76_RS02830 0.38 -0.4 578,732 + BWI76_RS02830 0.47 +1.5 578,920 + BWI76_RS02830 0.62 -0.7 578,920 + BWI76_RS02830 0.62 -0.9 578,921 + BWI76_RS02830 0.62 -0.6 578,922 + BWI76_RS02830 0.62 -1.1 578,930 - BWI76_RS02830 0.63 -0.3 578,930 - BWI76_RS02830 0.63 -1.5 578,930 - BWI76_RS02830 0.63 -0.0 578,930 - BWI76_RS02830 0.63 -0.8 579,006 - BWI76_RS02830 0.69 -1.7 579,079 - BWI76_RS02830 0.75 -0.0 579,110 - BWI76_RS02830 0.78 -0.3 579,120 - BWI76_RS02830 0.79 -0.1 579,136 + BWI76_RS02830 0.80 -0.9 579,144 - BWI76_RS02830 0.81 -0.5 579,144 - BWI76_RS02830 0.81 +0.0 579,144 - BWI76_RS02830 0.81 -1.6 579,144 - BWI76_RS02830 0.81 +1.6 579,144 - BWI76_RS02830 0.81 -0.5 579,144 - BWI76_RS02830 0.81 +0.3 579,144 - BWI76_RS02830 0.81 -0.7 579,153 + BWI76_RS02830 0.81 -0.5 579,156 - BWI76_RS02830 0.82 -0.4 579,169 - BWI76_RS02830 0.83 -0.9 579,172 + BWI76_RS02830 0.83 -0.4 579,240 + BWI76_RS02830 0.89 -1.9 579,302 - +1.1 579,340 - +0.0 579,354 - +0.5 579,398 + -0.3 579,453 + -0.8 579,453 + -1.2 579,453 + -0.8 579,453 + +0.6 579,461 - +0.4 579,462 - +0.1 579,462 - +0.8 579,463 - -0.6 579,576 + +1.3 579,585 + -0.7 579,585 + -0.4 579,586 + +0.7 579,591 + +0.1 579,593 - -1.7 579,598 + +0.3 579,598 + -2.8 579,601 + +0.0 579,601 + -0.0 579,603 + +0.6 579,604 - -1.4 579,609 - -1.3 579,619 - -0.8 579,619 - -1.0 579,619 - +0.4 579,627 - -1.8 579,628 - +2.4 579,634 + -0.7 579,653 + +0.9 579,666 + -0.3 579,666 - -0.3 579,668 + +2.1 579,668 + -3.4 579,668 + -0.3 579,676 - -0.7 579,676 - +0.9 579,676 - +0.4 579,676 - +0.2 579,676 - +0.8 579,719 - -1.5 579,725 + +0.3 579,726 + +0.5 579,726 + +1.0 579,729 - +0.1 579,729 - +0.2 579,734 - +0.7 579,734 - -0.1 579,765 + +0.4 579,767 - +1.1 579,769 + -0.2 579,769 + +0.4 579,770 + +0.2 579,777 + -1.1 579,778 - -0.3 579,779 - -1.8 579,784 - -0.6 579,784 - -1.4 579,812 + -1.4 579,842 + BWI76_RS02835 0.12 +0.4 579,843 - BWI76_RS02835 0.12 +0.2 579,862 + BWI76_RS02835 0.14 -0.3 579,862 + BWI76_RS02835 0.14 -0.0 579,862 + BWI76_RS02835 0.14 -0.9 579,862 + BWI76_RS02835 0.14 -2.4 579,870 - BWI76_RS02835 0.15 -0.1 579,870 - BWI76_RS02835 0.15 -0.5 579,870 - BWI76_RS02835 0.15 -0.1 579,870 - BWI76_RS02835 0.15 -0.8 579,870 - BWI76_RS02835 0.15 +0.6 579,888 - BWI76_RS02835 0.16 -1.7 579,892 + BWI76_RS02835 0.16 +0.5 579,912 + BWI76_RS02835 0.18 -0.0 579,919 + BWI76_RS02835 0.19 -1.1 579,927 - BWI76_RS02835 0.20 -0.8 579,939 + BWI76_RS02835 0.21 +0.0 579,995 - BWI76_RS02835 0.25 -0.3 580,015 + BWI76_RS02835 0.27 -2.5 580,015 + BWI76_RS02835 0.27 -0.8 580,015 + BWI76_RS02835 0.27 -0.6 580,021 + BWI76_RS02835 0.28 +0.3 580,044 + BWI76_RS02835 0.30 -0.3 580,063 + BWI76_RS02835 0.31 +0.1 580,064 + BWI76_RS02835 0.31 -1.2 580,076 - BWI76_RS02835 0.33 -1.2 580,080 + BWI76_RS02835 0.33 -0.7 580,080 + BWI76_RS02835 0.33 -1.3 580,080 + BWI76_RS02835 0.33 -0.5 580,080 + BWI76_RS02835 0.33 -3.1 580,088 - BWI76_RS02835 0.34 +0.6 580,095 + BWI76_RS02835 0.34 +0.1 580,105 + BWI76_RS02835 0.35 -1.0 580,105 + BWI76_RS02835 0.35 +0.6 580,105 + BWI76_RS02835 0.35 +0.1 580,105 + BWI76_RS02835 0.35 +3.8 580,113 - BWI76_RS02835 0.36 -0.5 580,113 - BWI76_RS02835 0.36 +0.8 580,118 + BWI76_RS02835 0.36 +0.8 580,118 + BWI76_RS02835 0.36 +0.0 580,118 + BWI76_RS02835 0.36 -1.7 580,118 + BWI76_RS02835 0.36 +0.4 580,126 - BWI76_RS02835 0.37 +0.2 580,126 - BWI76_RS02835 0.37 +0.1 580,126 - BWI76_RS02835 0.37 +0.6 580,126 - BWI76_RS02835 0.37 +0.9 580,144 + BWI76_RS02835 0.38 +0.2 580,149 + BWI76_RS02835 0.39 +0.6 580,175 + BWI76_RS02835 0.41 -0.9 580,183 - BWI76_RS02835 0.42 +0.7 580,186 + BWI76_RS02835 0.42 -0.0 580,220 + BWI76_RS02835 0.45 +1.3 580,252 - BWI76_RS02835 0.48 -0.9 580,252 - BWI76_RS02835 0.48 +0.3 580,252 - BWI76_RS02835 0.48 -0.6 580,252 - BWI76_RS02835 0.48 +0.3 580,252 - BWI76_RS02835 0.48 +1.0 580,254 + BWI76_RS02835 0.48 -1.3 580,262 - BWI76_RS02835 0.49 -0.5 580,267 + BWI76_RS02835 0.49 +1.5 580,268 + BWI76_RS02835 0.49 -0.4 580,268 + BWI76_RS02835 0.49 +0.2 580,268 + BWI76_RS02835 0.49 +0.4 580,275 - BWI76_RS02835 0.50 +2.7 580,277 + BWI76_RS02835 0.50 -0.1 580,277 + BWI76_RS02835 0.50 -0.0 580,359 + BWI76_RS02835 0.57 +0.3
Or see this region's nucleotide sequence