Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16460 and BWI76_RS16465 are separated by 48 nucleotides BWI76_RS16465 and BWI76_RS16470 are separated by 142 nucleotides BWI76_RS16470 and BWI76_RS16475 are separated by 53 nucleotides
BWI76_RS16460: BWI76_RS16460 - oxidoreductase, at 3,414,116 to 3,415,012
_RS16460
BWI76_RS16465: BWI76_RS16465 - DUF1289 domain-containing protein, at 3,415,061 to 3,415,300
_RS16465
BWI76_RS16470: BWI76_RS16470 - transcriptional repressor NemR, at 3,415,443 to 3,416,042
_RS16470
BWI76_RS16475: BWI76_RS16475 - alkene reductase, at 3,416,096 to 3,417,193
_RS16475
Position (kb)
3415
3416
3417 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3414.814 kb on - strand, within BWI76_RS16460 at 3414.983 kb on + strand at 3415.448 kb on + strand at 3415.499 kb on + strand at 3415.619 kb on + strand, within BWI76_RS16470 at 3416.082 kb on + strand at 3416.187 kb on + strand at 3416.195 kb on - strand at 3416.213 kb on - strand, within BWI76_RS16475 at 3416.290 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.333 kb on - strand, within BWI76_RS16475 at 3416.717 kb on + strand, within BWI76_RS16475 at 3416.725 kb on - strand, within BWI76_RS16475 at 3417.036 kb on + strand, within BWI76_RS16475
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 3,414,814 - BWI76_RS16460 0.78 -0.8 3,414,983 + -0.7 3,415,448 + -0.7 3,415,499 + +0.2 3,415,619 + BWI76_RS16470 0.29 -1.0 3,416,082 + +0.7 3,416,187 + +1.1 3,416,195 - -1.9 3,416,213 - BWI76_RS16475 0.11 -0.6 3,416,290 + BWI76_RS16475 0.18 +0.0 3,416,325 + BWI76_RS16475 0.21 +0.5 3,416,325 + BWI76_RS16475 0.21 -0.6 3,416,333 - BWI76_RS16475 0.22 -0.5 3,416,717 + BWI76_RS16475 0.57 +1.0 3,416,725 - BWI76_RS16475 0.57 +0.5 3,417,036 + BWI76_RS16475 0.86 -2.8
Or see this region's nucleotide sequence