Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14275 and BWI76_RS14280 are separated by 74 nucleotides BWI76_RS14280 and BWI76_RS14285 are separated by 310 nucleotides BWI76_RS14285 and BWI76_RS14290 are separated by 42 nucleotides
BWI76_RS14275: BWI76_RS14275 - hypothetical protein, at 2,985,593 to 2,986,789
_RS14275
BWI76_RS14280: BWI76_RS14280 - hypothetical protein, at 2,986,864 to 2,987,121
_RS14280
BWI76_RS14285: BWI76_RS14285 - IS3 family transposase, at 2,987,432 to 2,987,722
_RS14285
BWI76_RS14290: BWI76_RS14290 - AAA family ATPase, at 2,987,765 to 2,988,368
_RS14290
Position (kb)
2987
2988 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2986.439 kb on - strand, within BWI76_RS14275 at 2986.457 kb on - strand, within BWI76_RS14275 at 2986.542 kb on - strand, within BWI76_RS14275 at 2986.613 kb on - strand, within BWI76_RS14275 at 2986.614 kb on + strand, within BWI76_RS14275 at 2986.839 kb on - strand at 2986.875 kb on + strand at 2986.942 kb on + strand, within BWI76_RS14280 at 2986.993 kb on + strand, within BWI76_RS14280 at 2987.043 kb on - strand, within BWI76_RS14280 at 2987.173 kb on + strand at 2987.185 kb on - strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.194 kb on + strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.202 kb on - strand at 2987.250 kb on - strand at 2987.275 kb on + strand at 2987.276 kb on + strand at 2987.293 kb on - strand at 2987.401 kb on + strand at 2987.446 kb on + strand at 2987.481 kb on - strand, within BWI76_RS14285 at 2987.491 kb on - strand, within BWI76_RS14285 at 2987.514 kb on - strand, within BWI76_RS14285 at 2987.520 kb on + strand, within BWI76_RS14285 at 2987.524 kb on + strand, within BWI76_RS14285 at 2987.534 kb on + strand, within BWI76_RS14285 at 2987.534 kb on + strand, within BWI76_RS14285 at 2987.878 kb on + strand, within BWI76_RS14290 at 2987.886 kb on - strand, within BWI76_RS14290 at 2987.898 kb on + strand, within BWI76_RS14290 at 2987.921 kb on + strand, within BWI76_RS14290 at 2987.955 kb on + strand, within BWI76_RS14290 at 2988.029 kb on + strand, within BWI76_RS14290 at 2988.079 kb on + strand, within BWI76_RS14290 at 2988.231 kb on - strand, within BWI76_RS14290 at 2988.344 kb on + strand at 2988.463 kb on - strand at 2988.472 kb on - strand at 2988.516 kb on + strand at 2988.531 kb on - strand at 2988.586 kb on - strand at 2988.596 kb on - strand at 2988.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 2,986,439 - BWI76_RS14275 0.71 +0.3 2,986,457 - BWI76_RS14275 0.72 -0.3 2,986,542 - BWI76_RS14275 0.79 +0.5 2,986,613 - BWI76_RS14275 0.85 -0.7 2,986,614 + BWI76_RS14275 0.85 -1.9 2,986,839 - -0.8 2,986,875 + +0.1 2,986,942 + BWI76_RS14280 0.30 +0.2 2,986,993 + BWI76_RS14280 0.50 -0.0 2,987,043 - BWI76_RS14280 0.69 -0.7 2,987,173 + -0.4 2,987,185 - -2.1 2,987,194 + +0.8 2,987,194 + -0.4 2,987,194 + -0.7 2,987,194 + -0.4 2,987,194 + -0.3 2,987,194 + -1.0 2,987,194 + +0.7 2,987,194 + +0.3 2,987,194 + -1.3 2,987,194 + -1.0 2,987,194 + -1.1 2,987,194 + -2.8 2,987,194 + -0.8 2,987,194 + -0.1 2,987,194 + +0.3 2,987,202 - -0.1 2,987,202 - +0.5 2,987,202 - -1.3 2,987,202 - -0.1 2,987,202 - +0.9 2,987,202 - +0.6 2,987,202 - -2.1 2,987,202 - -0.1 2,987,202 - -0.6 2,987,202 - +0.4 2,987,202 - +0.2 2,987,250 - -0.2 2,987,275 + +0.4 2,987,276 + -0.0 2,987,293 - -0.2 2,987,401 + -0.2 2,987,446 + -1.2 2,987,481 - BWI76_RS14285 0.17 -1.6 2,987,491 - BWI76_RS14285 0.20 -2.5 2,987,514 - BWI76_RS14285 0.28 -1.4 2,987,520 + BWI76_RS14285 0.30 -0.1 2,987,524 + BWI76_RS14285 0.32 -0.3 2,987,534 + BWI76_RS14285 0.35 -0.8 2,987,534 + BWI76_RS14285 0.35 -0.3 2,987,878 + BWI76_RS14290 0.19 -0.5 2,987,886 - BWI76_RS14290 0.20 -0.8 2,987,898 + BWI76_RS14290 0.22 +0.9 2,987,921 + BWI76_RS14290 0.26 -0.1 2,987,955 + BWI76_RS14290 0.31 +1.1 2,988,029 + BWI76_RS14290 0.44 +0.9 2,988,079 + BWI76_RS14290 0.52 +1.4 2,988,231 - BWI76_RS14290 0.77 +0.8 2,988,344 + -2.0 2,988,463 - -1.1 2,988,472 - +0.0 2,988,516 + -1.2 2,988,531 - -1.4 2,988,586 - +0.3 2,988,596 - -0.1 2,988,621 - +0.1
Or see this region's nucleotide sequence