Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18610 and BWI76_RS18615 are separated by 213 nucleotides BWI76_RS18615 and BWI76_RS18620 are separated by 102 nucleotides BWI76_RS18620 and BWI76_RS18625 are separated by 78 nucleotides
BWI76_RS18610: BWI76_RS18610 - transcriptional regulator LysR, at 3,858,896 to 3,859,882
_RS18610
BWI76_RS18615: BWI76_RS18615 - glutathione S-transferase domain protein, at 3,860,096 to 3,860,704
_RS18615
BWI76_RS18620: BWI76_RS18620 - L,D-transpeptidase, at 3,860,807 to 3,861,748
_RS18620
BWI76_RS18625: BWI76_RS18625 - CysB family transcriptional regulator, at 3,861,827 to 3,862,777
_RS18625
Position (kb)
3860
3861
3862 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3859.972 kb on + strand at 3860.006 kb on + strand at 3860.006 kb on + strand at 3860.014 kb on + strand at 3860.016 kb on - strand at 3860.038 kb on - strand at 3860.338 kb on + strand, within BWI76_RS18615 at 3860.362 kb on + strand, within BWI76_RS18615 at 3860.387 kb on - strand, within BWI76_RS18615 at 3860.477 kb on - strand, within BWI76_RS18615 at 3860.492 kb on - strand, within BWI76_RS18615 at 3860.520 kb on - strand, within BWI76_RS18615 at 3860.558 kb on - strand, within BWI76_RS18615 at 3860.560 kb on + strand, within BWI76_RS18615 at 3860.617 kb on + strand at 3860.705 kb on + strand at 3860.713 kb on - strand at 3860.757 kb on - strand at 3860.867 kb on + strand at 3860.966 kb on + strand, within BWI76_RS18620 at 3861.131 kb on + strand, within BWI76_RS18620 at 3861.139 kb on - strand, within BWI76_RS18620 at 3861.321 kb on + strand, within BWI76_RS18620 at 3861.481 kb on + strand, within BWI76_RS18620 at 3861.506 kb on + strand, within BWI76_RS18620 at 3861.526 kb on + strand, within BWI76_RS18620 at 3861.534 kb on - strand, within BWI76_RS18620 at 3861.534 kb on - strand, within BWI76_RS18620 at 3861.534 kb on - strand, within BWI76_RS18620 at 3861.535 kb on - strand, within BWI76_RS18620 at 3861.575 kb on + strand, within BWI76_RS18620 at 3861.594 kb on + strand at 3861.675 kb on + strand at 3861.723 kb on + strand at 3861.723 kb on + strand at 3861.839 kb on + strand at 3861.980 kb on - strand, within BWI76_RS18625 at 3862.062 kb on - strand, within BWI76_RS18625 at 3862.090 kb on - strand, within BWI76_RS18625 at 3862.142 kb on + strand, within BWI76_RS18625 at 3862.148 kb on + strand, within BWI76_RS18625 at 3862.150 kb on - strand, within BWI76_RS18625 at 3862.150 kb on - strand, within BWI76_RS18625 at 3862.399 kb on - strand, within BWI76_RS18625 at 3862.400 kb on - strand, within BWI76_RS18625 at 3862.434 kb on - strand, within BWI76_RS18625 at 3862.457 kb on + strand, within BWI76_RS18625 at 3862.472 kb on + strand, within BWI76_RS18625 at 3862.485 kb on - strand, within BWI76_RS18625 at 3862.584 kb on + strand, within BWI76_RS18625 at 3862.685 kb on + strand at 3862.695 kb on + strand at 3862.703 kb on - strand at 3862.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 3,859,972 + -0.3 3,860,006 + +0.3 3,860,006 + +0.4 3,860,014 + -0.2 3,860,016 - -1.2 3,860,038 - -1.0 3,860,338 + BWI76_RS18615 0.40 -1.1 3,860,362 + BWI76_RS18615 0.44 +1.4 3,860,387 - BWI76_RS18615 0.48 -2.0 3,860,477 - BWI76_RS18615 0.63 +0.6 3,860,492 - BWI76_RS18615 0.65 -1.4 3,860,520 - BWI76_RS18615 0.70 -3.4 3,860,558 - BWI76_RS18615 0.76 -3.0 3,860,560 + BWI76_RS18615 0.76 +0.3 3,860,617 + +0.3 3,860,705 + -0.6 3,860,713 - +0.3 3,860,757 - +1.2 3,860,867 + +1.5 3,860,966 + BWI76_RS18620 0.17 +0.2 3,861,131 + BWI76_RS18620 0.34 -1.3 3,861,139 - BWI76_RS18620 0.35 -1.1 3,861,321 + BWI76_RS18620 0.55 -2.1 3,861,481 + BWI76_RS18620 0.72 +0.7 3,861,506 + BWI76_RS18620 0.74 -1.0 3,861,526 + BWI76_RS18620 0.76 -1.5 3,861,534 - BWI76_RS18620 0.77 -1.2 3,861,534 - BWI76_RS18620 0.77 +0.3 3,861,534 - BWI76_RS18620 0.77 -1.5 3,861,535 - BWI76_RS18620 0.77 -0.6 3,861,575 + BWI76_RS18620 0.82 -0.8 3,861,594 + +2.9 3,861,675 + +0.6 3,861,723 + -1.1 3,861,723 + -0.1 3,861,839 + -0.6 3,861,980 - BWI76_RS18625 0.16 -0.6 3,862,062 - BWI76_RS18625 0.25 +0.6 3,862,090 - BWI76_RS18625 0.28 -0.3 3,862,142 + BWI76_RS18625 0.33 -0.2 3,862,148 + BWI76_RS18625 0.34 -0.2 3,862,150 - BWI76_RS18625 0.34 -0.8 3,862,150 - BWI76_RS18625 0.34 +1.2 3,862,399 - BWI76_RS18625 0.60 +0.1 3,862,400 - BWI76_RS18625 0.60 +0.7 3,862,434 - BWI76_RS18625 0.64 -0.4 3,862,457 + BWI76_RS18625 0.66 +2.9 3,862,472 + BWI76_RS18625 0.68 -0.7 3,862,485 - BWI76_RS18625 0.69 -0.4 3,862,584 + BWI76_RS18625 0.80 -0.2 3,862,685 + -0.4 3,862,695 + +0.0 3,862,703 - -0.0 3,862,724 - -0.3
Or see this region's nucleotide sequence