Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18620

Experiment: K. M5a1 KLEB10 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18610 and BWI76_RS18615 are separated by 213 nucleotidesBWI76_RS18615 and BWI76_RS18620 are separated by 102 nucleotidesBWI76_RS18620 and BWI76_RS18625 are separated by 78 nucleotides BWI76_RS18610: BWI76_RS18610 - transcriptional regulator LysR, at 3,858,896 to 3,859,882 _RS18610 BWI76_RS18615: BWI76_RS18615 - glutathione S-transferase domain protein, at 3,860,096 to 3,860,704 _RS18615 BWI76_RS18620: BWI76_RS18620 - L,D-transpeptidase, at 3,860,807 to 3,861,748 _RS18620 BWI76_RS18625: BWI76_RS18625 - CysB family transcriptional regulator, at 3,861,827 to 3,862,777 _RS18625 Position (kb) 3860 3861 3862Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3859.972 kb on + strandat 3860.006 kb on + strandat 3860.006 kb on + strandat 3860.014 kb on + strandat 3860.016 kb on - strandat 3860.038 kb on - strandat 3860.338 kb on + strand, within BWI76_RS18615at 3860.362 kb on + strand, within BWI76_RS18615at 3860.387 kb on - strand, within BWI76_RS18615at 3860.477 kb on - strand, within BWI76_RS18615at 3860.492 kb on - strand, within BWI76_RS18615at 3860.520 kb on - strand, within BWI76_RS18615at 3860.558 kb on - strand, within BWI76_RS18615at 3860.560 kb on + strand, within BWI76_RS18615at 3860.617 kb on + strandat 3860.705 kb on + strandat 3860.713 kb on - strandat 3860.757 kb on - strandat 3860.867 kb on + strandat 3860.966 kb on + strand, within BWI76_RS18620at 3861.131 kb on + strand, within BWI76_RS18620at 3861.139 kb on - strand, within BWI76_RS18620at 3861.321 kb on + strand, within BWI76_RS18620at 3861.481 kb on + strand, within BWI76_RS18620at 3861.506 kb on + strand, within BWI76_RS18620at 3861.526 kb on + strand, within BWI76_RS18620at 3861.534 kb on - strand, within BWI76_RS18620at 3861.534 kb on - strand, within BWI76_RS18620at 3861.534 kb on - strand, within BWI76_RS18620at 3861.535 kb on - strand, within BWI76_RS18620at 3861.575 kb on + strand, within BWI76_RS18620at 3861.594 kb on + strandat 3861.675 kb on + strandat 3861.723 kb on + strandat 3861.723 kb on + strandat 3861.839 kb on + strandat 3861.980 kb on - strand, within BWI76_RS18625at 3862.062 kb on - strand, within BWI76_RS18625at 3862.090 kb on - strand, within BWI76_RS18625at 3862.142 kb on + strand, within BWI76_RS18625at 3862.148 kb on + strand, within BWI76_RS18625at 3862.150 kb on - strand, within BWI76_RS18625at 3862.150 kb on - strand, within BWI76_RS18625at 3862.399 kb on - strand, within BWI76_RS18625at 3862.400 kb on - strand, within BWI76_RS18625at 3862.434 kb on - strand, within BWI76_RS18625at 3862.457 kb on + strand, within BWI76_RS18625at 3862.472 kb on + strand, within BWI76_RS18625at 3862.485 kb on - strand, within BWI76_RS18625at 3862.584 kb on + strand, within BWI76_RS18625at 3862.685 kb on + strandat 3862.695 kb on + strandat 3862.703 kb on - strandat 3862.724 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10
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3,859,972 + -0.3
3,860,006 + +0.3
3,860,006 + +0.4
3,860,014 + -0.2
3,860,016 - -1.2
3,860,038 - -1.0
3,860,338 + BWI76_RS18615 0.40 -1.1
3,860,362 + BWI76_RS18615 0.44 +1.4
3,860,387 - BWI76_RS18615 0.48 -2.0
3,860,477 - BWI76_RS18615 0.63 +0.6
3,860,492 - BWI76_RS18615 0.65 -1.4
3,860,520 - BWI76_RS18615 0.70 -3.4
3,860,558 - BWI76_RS18615 0.76 -3.0
3,860,560 + BWI76_RS18615 0.76 +0.3
3,860,617 + +0.3
3,860,705 + -0.6
3,860,713 - +0.3
3,860,757 - +1.2
3,860,867 + +1.5
3,860,966 + BWI76_RS18620 0.17 +0.2
3,861,131 + BWI76_RS18620 0.34 -1.3
3,861,139 - BWI76_RS18620 0.35 -1.1
3,861,321 + BWI76_RS18620 0.55 -2.1
3,861,481 + BWI76_RS18620 0.72 +0.7
3,861,506 + BWI76_RS18620 0.74 -1.0
3,861,526 + BWI76_RS18620 0.76 -1.5
3,861,534 - BWI76_RS18620 0.77 -1.2
3,861,534 - BWI76_RS18620 0.77 +0.3
3,861,534 - BWI76_RS18620 0.77 -1.5
3,861,535 - BWI76_RS18620 0.77 -0.6
3,861,575 + BWI76_RS18620 0.82 -0.8
3,861,594 + +2.9
3,861,675 + +0.6
3,861,723 + -1.1
3,861,723 + -0.1
3,861,839 + -0.6
3,861,980 - BWI76_RS18625 0.16 -0.6
3,862,062 - BWI76_RS18625 0.25 +0.6
3,862,090 - BWI76_RS18625 0.28 -0.3
3,862,142 + BWI76_RS18625 0.33 -0.2
3,862,148 + BWI76_RS18625 0.34 -0.2
3,862,150 - BWI76_RS18625 0.34 -0.8
3,862,150 - BWI76_RS18625 0.34 +1.2
3,862,399 - BWI76_RS18625 0.60 +0.1
3,862,400 - BWI76_RS18625 0.60 +0.7
3,862,434 - BWI76_RS18625 0.64 -0.4
3,862,457 + BWI76_RS18625 0.66 +2.9
3,862,472 + BWI76_RS18625 0.68 -0.7
3,862,485 - BWI76_RS18625 0.69 -0.4
3,862,584 + BWI76_RS18625 0.80 -0.2
3,862,685 + -0.4
3,862,695 + +0.0
3,862,703 - -0.0
3,862,724 - -0.3

Or see this region's nucleotide sequence