Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17750 and BWI76_RS17755 are separated by 130 nucleotides BWI76_RS17755 and BWI76_RS17760 are separated by 118 nucleotides BWI76_RS17760 and BWI76_RS17765 are separated by 8 nucleotides BWI76_RS17765 and BWI76_RS17770 are separated by 94 nucleotides
BWI76_RS17750: BWI76_RS17750 - Na+/H+ antiporter NhaB, at 3,691,745 to 3,693,298
_RS17750
BWI76_RS17755: BWI76_RS17755 - disulfide bond formation protein B, at 3,693,429 to 3,693,959
_RS17755
BWI76_RS17760: BWI76_RS17760 - hypothetical protein, at 3,694,078 to 3,694,422
_RS17760
BWI76_RS17765: BWI76_RS17765 - hypothetical protein, at 3,694,431 to 3,694,877
_RS17765
BWI76_RS17770: BWI76_RS17770 - isomerase/hydrolase, at 3,694,972 to 3,695,631
_RS17770
Position (kb)
3694
3695 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3693.189 kb on + strand at 3693.397 kb on + strand at 3693.420 kb on - strand at 3693.451 kb on + strand at 3693.898 kb on + strand, within BWI76_RS17755 at 3694.273 kb on + strand, within BWI76_RS17760 at 3694.333 kb on - strand, within BWI76_RS17760 at 3694.611 kb on - strand, within BWI76_RS17765 at 3694.655 kb on - strand, within BWI76_RS17765 at 3694.948 kb on + strand at 3694.977 kb on + strand at 3695.186 kb on - strand, within BWI76_RS17770 at 3695.420 kb on + strand, within BWI76_RS17770
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 3,693,189 + -1.7 3,693,397 + +0.8 3,693,420 - +2.1 3,693,451 + +1.7 3,693,898 + BWI76_RS17755 0.88 -0.1 3,694,273 + BWI76_RS17760 0.57 -2.2 3,694,333 - BWI76_RS17760 0.74 -0.1 3,694,611 - BWI76_RS17765 0.40 +0.1 3,694,655 - BWI76_RS17765 0.50 +0.6 3,694,948 + +0.4 3,694,977 + +0.5 3,695,186 - BWI76_RS17770 0.32 +0.7 3,695,420 + BWI76_RS17770 0.68 -0.4
Or see this region's nucleotide sequence