Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15345 and BWI76_RS15350 are separated by 149 nucleotides BWI76_RS15350 and BWI76_RS15355 are separated by 67 nucleotides BWI76_RS15355 and BWI76_RS15360 are separated by 423 nucleotides BWI76_RS15360 and BWI76_RS15365 are separated by 88 nucleotides
BWI76_RS15345: BWI76_RS15345 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 3,214,024 to 3,215,127
_RS15345
BWI76_RS15350: BWI76_RS15350 - hypothetical protein, at 3,215,277 to 3,215,669
_RS15350
BWI76_RS15355: BWI76_RS15355 - transcriptional regulator FtrA, at 3,215,737 to 3,216,747
_RS15355
BWI76_RS15360: BWI76_RS15360 - hypothetical protein, at 3,217,171 to 3,217,506
_RS15360
BWI76_RS15365: BWI76_RS15365 - hypothetical protein, at 3,217,595 to 3,217,927
_RS15365
Position (kb)
3215
3216
3217 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3214.844 kb on - strand, within BWI76_RS15345 at 3215.104 kb on + strand at 3215.295 kb on + strand at 3215.297 kb on - strand at 3215.303 kb on - strand at 3215.354 kb on - strand, within BWI76_RS15350 at 3216.042 kb on + strand, within BWI76_RS15355 at 3216.052 kb on + strand, within BWI76_RS15355 at 3216.220 kb on + strand, within BWI76_RS15355 at 3216.228 kb on - strand, within BWI76_RS15355 at 3216.510 kb on - strand, within BWI76_RS15355 at 3216.603 kb on - strand, within BWI76_RS15355 at 3216.604 kb on - strand, within BWI76_RS15355 at 3216.609 kb on - strand at 3216.617 kb on + strand at 3216.617 kb on + strand, within BWI76_RS15355 at 3216.625 kb on - strand, within BWI76_RS15355 at 3216.625 kb on - strand, within BWI76_RS15355 at 3216.625 kb on - strand, within BWI76_RS15355 at 3216.654 kb on + strand at 3216.669 kb on - strand at 3216.727 kb on - strand at 3216.805 kb on + strand at 3216.806 kb on + strand at 3216.811 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.834 kb on + strand at 3216.843 kb on - strand at 3216.844 kb on + strand at 3216.844 kb on + strand at 3216.844 kb on + strand at 3216.844 kb on + strand at 3216.844 kb on + strand at 3216.852 kb on - strand at 3216.875 kb on + strand at 3216.875 kb on + strand at 3216.985 kb on + strand at 3216.998 kb on + strand at 3217.026 kb on + strand at 3217.033 kb on - strand at 3217.034 kb on - strand at 3217.034 kb on - strand at 3217.080 kb on - strand at 3217.106 kb on - strand at 3217.123 kb on - strand at 3217.226 kb on + strand, within BWI76_RS15360 at 3217.229 kb on - strand, within BWI76_RS15360 at 3217.234 kb on - strand, within BWI76_RS15360 at 3217.293 kb on + strand, within BWI76_RS15360 at 3217.293 kb on + strand, within BWI76_RS15360 at 3217.463 kb on + strand, within BWI76_RS15360
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 3,214,844 - BWI76_RS15345 0.74 +0.8 3,215,104 + +1.2 3,215,295 + +0.8 3,215,297 - +0.6 3,215,303 - +0.2 3,215,354 - BWI76_RS15350 0.20 -3.7 3,216,042 + BWI76_RS15355 0.30 -0.3 3,216,052 + BWI76_RS15355 0.31 +1.2 3,216,220 + BWI76_RS15355 0.48 +1.7 3,216,228 - BWI76_RS15355 0.49 +0.4 3,216,510 - BWI76_RS15355 0.76 -0.4 3,216,603 - BWI76_RS15355 0.86 -3.3 3,216,604 - BWI76_RS15355 0.86 +2.0 3,216,609 - -1.3 3,216,617 + +0.4 3,216,617 + BWI76_RS15355 0.87 -0.0 3,216,625 - BWI76_RS15355 0.88 +0.1 3,216,625 - BWI76_RS15355 0.88 +2.2 3,216,625 - BWI76_RS15355 0.88 +0.5 3,216,654 + -2.2 3,216,669 - -0.1 3,216,727 - +0.8 3,216,805 + +2.0 3,216,806 + -0.0 3,216,811 + -0.5 3,216,825 + +0.8 3,216,825 + +1.0 3,216,825 + +2.2 3,216,825 + -0.0 3,216,825 + +1.1 3,216,825 + -2.1 3,216,833 - +1.9 3,216,833 - -1.2 3,216,833 - -1.3 3,216,833 - -2.3 3,216,833 - -1.2 3,216,833 - -0.2 3,216,834 + -1.1 3,216,843 - -1.3 3,216,844 + -2.0 3,216,844 + -0.6 3,216,844 + -0.3 3,216,844 + -1.1 3,216,844 + +0.2 3,216,852 - +0.6 3,216,875 + +0.4 3,216,875 + +0.2 3,216,985 + -0.3 3,216,998 + +0.5 3,217,026 + +0.5 3,217,033 - +0.1 3,217,034 - -1.8 3,217,034 - -1.1 3,217,080 - +0.4 3,217,106 - -0.8 3,217,123 - +0.7 3,217,226 + BWI76_RS15360 0.16 -0.7 3,217,229 - BWI76_RS15360 0.17 -0.2 3,217,234 - BWI76_RS15360 0.19 +1.0 3,217,293 + BWI76_RS15360 0.36 +2.1 3,217,293 + BWI76_RS15360 0.36 -2.0 3,217,463 + BWI76_RS15360 0.87 +0.4
Or see this region's nucleotide sequence