Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15305 and BWI76_RS15310 are separated by 192 nucleotides BWI76_RS15310 and BWI76_RS15315 are separated by 117 nucleotides BWI76_RS15315 and BWI76_RS15320 are separated by 115 nucleotides
BWI76_RS15305: BWI76_RS15305 - O-acetylserine/cysteine exporter, at 3,205,350 to 3,206,249
_RS15305
BWI76_RS15310: BWI76_RS15310 - MFS transporter, at 3,206,442 to 3,207,635
_RS15310
BWI76_RS15315: BWI76_RS15315 - DNA-binding protein, at 3,207,753 to 3,208,223
_RS15315
BWI76_RS15320: BWI76_RS15320 - ABC transporter ATP-binding protein, at 3,208,339 to 3,209,037
_RS15320
Position (kb)
3206
3207
3208 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3205.560 kb on - strand, within BWI76_RS15305 at 3205.788 kb on + strand, within BWI76_RS15305 at 3206.111 kb on - strand, within BWI76_RS15305 at 3206.314 kb on + strand at 3206.379 kb on - strand at 3206.525 kb on - strand at 3206.717 kb on + strand, within BWI76_RS15310 at 3206.762 kb on - strand, within BWI76_RS15310 at 3206.764 kb on - strand, within BWI76_RS15310 at 3206.925 kb on - strand, within BWI76_RS15310 at 3207.014 kb on + strand at 3207.022 kb on - strand, within BWI76_RS15310 at 3207.070 kb on + strand, within BWI76_RS15310 at 3207.071 kb on - strand, within BWI76_RS15310 at 3207.073 kb on - strand, within BWI76_RS15310 at 3207.078 kb on - strand, within BWI76_RS15310 at 3207.282 kb on - strand, within BWI76_RS15310 at 3207.338 kb on + strand, within BWI76_RS15310 at 3207.361 kb on + strand, within BWI76_RS15310 at 3207.711 kb on + strand at 3207.730 kb on - strand at 3207.952 kb on + strand, within BWI76_RS15315 at 3207.960 kb on - strand, within BWI76_RS15315 at 3207.987 kb on + strand at 3207.995 kb on - strand, within BWI76_RS15315
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 3,205,560 - BWI76_RS15305 0.23 +1.8 3,205,788 + BWI76_RS15305 0.49 -2.1 3,206,111 - BWI76_RS15305 0.85 +0.7 3,206,314 + +0.9 3,206,379 - -0.6 3,206,525 - +0.0 3,206,717 + BWI76_RS15310 0.23 +0.6 3,206,762 - BWI76_RS15310 0.27 +0.4 3,206,764 - BWI76_RS15310 0.27 +0.1 3,206,925 - BWI76_RS15310 0.40 -3.2 3,207,014 + -0.0 3,207,022 - BWI76_RS15310 0.49 -1.9 3,207,070 + BWI76_RS15310 0.53 +0.6 3,207,071 - BWI76_RS15310 0.53 +1.2 3,207,073 - BWI76_RS15310 0.53 +1.0 3,207,078 - BWI76_RS15310 0.53 -0.7 3,207,282 - BWI76_RS15310 0.70 +0.7 3,207,338 + BWI76_RS15310 0.75 -2.3 3,207,361 + BWI76_RS15310 0.77 +2.4 3,207,711 + -0.8 3,207,730 - -0.6 3,207,952 + BWI76_RS15315 0.42 -2.8 3,207,960 - BWI76_RS15315 0.44 -0.1 3,207,987 + +1.0 3,207,995 - BWI76_RS15315 0.51 +0.2
Or see this region's nucleotide sequence