Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14965 and BWI76_RS14970 overlap by 4 nucleotides BWI76_RS14970 and BWI76_RS14975 are separated by 12 nucleotides BWI76_RS14975 and BWI76_RS14980 overlap by 4 nucleotides
BWI76_RS14965: BWI76_RS14965 - AraC family transcriptional regulator, at 3,137,120 to 3,138,076
_RS14965
BWI76_RS14970: BWI76_RS14970 - pyrroline-5-carboxylate reductase, at 3,138,073 to 3,138,861
_RS14970
BWI76_RS14975: BWI76_RS14975 - glyoxalase-like domain protein, at 3,138,874 to 3,139,629
_RS14975
BWI76_RS14980: BWI76_RS14980 - hypothetical protein, at 3,139,626 to 3,140,156
_RS14980
Position (kb)
3138
3139 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3137.141 kb on - strand at 3137.265 kb on + strand, within BWI76_RS14965 at 3137.467 kb on + strand, within BWI76_RS14965 at 3137.507 kb on - strand, within BWI76_RS14965 at 3137.932 kb on - strand, within BWI76_RS14965 at 3137.985 kb on - strand at 3138.616 kb on - strand, within BWI76_RS14970 at 3139.204 kb on + strand, within BWI76_RS14975 at 3139.430 kb on + strand, within BWI76_RS14975 at 3139.477 kb on + strand, within BWI76_RS14975 at 3139.643 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 3,137,141 - +0.6 3,137,265 + BWI76_RS14965 0.15 -3.3 3,137,467 + BWI76_RS14965 0.36 -1.6 3,137,507 - BWI76_RS14965 0.40 -2.0 3,137,932 - BWI76_RS14965 0.85 -0.4 3,137,985 - +0.3 3,138,616 - BWI76_RS14970 0.69 -2.7 3,139,204 + BWI76_RS14975 0.44 -0.1 3,139,430 + BWI76_RS14975 0.74 -0.2 3,139,477 + BWI76_RS14975 0.80 -0.6 3,139,643 + -0.6
Or see this region's nucleotide sequence