Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13100 and BWI76_RS13105 are separated by 12 nucleotides BWI76_RS13105 and BWI76_RS13110 are separated by 7 nucleotides BWI76_RS13110 and BWI76_RS13115 overlap by 4 nucleotides BWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotides
BWI76_RS13100: BWI76_RS13100 - phenylacetate-CoA oxygenase subunit PaaI, at 2,733,026 to 2,733,781
_RS13100
BWI76_RS13105: BWI76_RS13105 - phenylacetate-CoA oxygenase subunit PaaJ, at 2,733,794 to 2,734,291
_RS13105
BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369
_RS13110
BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133
_RS13115
BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924
_RS13120
Position (kb)
2734
2735
2736 Strain fitness (log2 ratio)
-1
0
1 at 2733.362 kb on + strand, within BWI76_RS13100 at 2733.402 kb on + strand, within BWI76_RS13100 at 2733.453 kb on + strand, within BWI76_RS13100 at 2733.541 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100 at 2733.765 kb on - strand at 2734.083 kb on + strand, within BWI76_RS13105 at 2734.176 kb on - strand, within BWI76_RS13105 at 2734.235 kb on + strand, within BWI76_RS13105 at 2734.454 kb on + strand, within BWI76_RS13110 at 2734.932 kb on - strand, within BWI76_RS13110 at 2735.127 kb on - strand, within BWI76_RS13110 at 2735.337 kb on + strand at 2735.375 kb on - strand at 2736.150 kb on - strand at 2736.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 2,733,362 + BWI76_RS13100 0.44 +1.1 2,733,402 + BWI76_RS13100 0.50 +0.6 2,733,453 + BWI76_RS13100 0.56 -0.6 2,733,541 + BWI76_RS13100 0.68 +1.2 2,733,580 + BWI76_RS13100 0.73 +0.8 2,733,580 + BWI76_RS13100 0.73 -0.8 2,733,765 - +0.1 2,734,083 + BWI76_RS13105 0.58 -0.1 2,734,176 - BWI76_RS13105 0.77 +0.8 2,734,235 + BWI76_RS13105 0.89 +0.2 2,734,454 + BWI76_RS13110 0.14 +1.4 2,734,932 - BWI76_RS13110 0.59 +0.3 2,735,127 - BWI76_RS13110 0.77 -0.0 2,735,337 + -0.2 2,735,375 - +0.2 2,736,150 - -0.4 2,736,166 - +0.7
Or see this region's nucleotide sequence