Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12295 and BWI76_RS12300 are separated by 22 nucleotides BWI76_RS12300 and BWI76_RS12305 are separated by 38 nucleotides BWI76_RS12305 and BWI76_RS12310 are separated by 137 nucleotides BWI76_RS12310 and BWI76_RS12315 are separated by 507 nucleotides
BWI76_RS12295: BWI76_RS12295 - hypothetical protein, at 2,567,206 to 2,567,598
_RS12295
BWI76_RS12300: BWI76_RS12300 - hypothetical protein, at 2,567,621 to 2,568,553
_RS12300
BWI76_RS12305: BWI76_RS12305 - hypothetical protein, at 2,568,592 to 2,569,074
_RS12305
BWI76_RS12310: BWI76_RS12310 - hypothetical protein, at 2,569,212 to 2,569,427
_RS12310
BWI76_RS12315: BWI76_RS12315 - hypothetical protein, at 2,569,935 to 2,570,459
_RS12315
Position (kb)
2568
2569
2570 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2567.674 kb on - strand at 2567.724 kb on + strand, within BWI76_RS12300 at 2567.724 kb on + strand, within BWI76_RS12300 at 2567.732 kb on - strand, within BWI76_RS12300 at 2567.788 kb on - strand, within BWI76_RS12300 at 2567.953 kb on + strand, within BWI76_RS12300 at 2568.147 kb on + strand, within BWI76_RS12300 at 2568.147 kb on - strand, within BWI76_RS12300 at 2568.162 kb on + strand, within BWI76_RS12300 at 2568.271 kb on + strand, within BWI76_RS12300 at 2568.305 kb on + strand, within BWI76_RS12300 at 2568.381 kb on + strand, within BWI76_RS12300 at 2568.496 kb on + strand at 2568.496 kb on + strand at 2568.504 kb on - strand at 2568.553 kb on + strand at 2568.793 kb on + strand, within BWI76_RS12305 at 2568.816 kb on + strand, within BWI76_RS12305 at 2568.905 kb on + strand, within BWI76_RS12305 at 2568.926 kb on + strand, within BWI76_RS12305 at 2568.937 kb on + strand, within BWI76_RS12305 at 2569.001 kb on - strand, within BWI76_RS12305 at 2569.032 kb on + strand at 2569.040 kb on - strand at 2569.087 kb on + strand at 2569.432 kb on + strand at 2569.459 kb on + strand at 2569.459 kb on + strand at 2569.467 kb on - strand at 2569.467 kb on - strand at 2569.467 kb on - strand at 2569.485 kb on + strand at 2569.485 kb on - strand at 2569.490 kb on + strand at 2569.490 kb on + strand at 2569.490 kb on + strand at 2569.498 kb on - strand at 2569.498 kb on - strand at 2569.733 kb on + strand at 2569.742 kb on - strand at 2569.760 kb on + strand at 2569.778 kb on + strand at 2569.868 kb on + strand at 2569.931 kb on + strand at 2569.937 kb on + strand at 2569.937 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 2,567,674 - +0.2 2,567,724 + BWI76_RS12300 0.11 -1.4 2,567,724 + BWI76_RS12300 0.11 +0.5 2,567,732 - BWI76_RS12300 0.12 -0.7 2,567,788 - BWI76_RS12300 0.18 -0.6 2,567,953 + BWI76_RS12300 0.36 -0.9 2,568,147 + BWI76_RS12300 0.56 +0.1 2,568,147 - BWI76_RS12300 0.56 -0.3 2,568,162 + BWI76_RS12300 0.58 -0.0 2,568,271 + BWI76_RS12300 0.70 -2.2 2,568,305 + BWI76_RS12300 0.73 -0.3 2,568,381 + BWI76_RS12300 0.81 +0.2 2,568,496 + -1.0 2,568,496 + +0.3 2,568,504 - -0.4 2,568,553 + -0.3 2,568,793 + BWI76_RS12305 0.42 -1.3 2,568,816 + BWI76_RS12305 0.46 -0.9 2,568,905 + BWI76_RS12305 0.65 -0.4 2,568,926 + BWI76_RS12305 0.69 -0.4 2,568,937 + BWI76_RS12305 0.71 -0.4 2,569,001 - BWI76_RS12305 0.85 -3.5 2,569,032 + -1.2 2,569,040 - -2.2 2,569,087 + -0.6 2,569,432 + -2.6 2,569,459 + -0.1 2,569,459 + +0.7 2,569,467 - -1.5 2,569,467 - -1.9 2,569,467 - +0.1 2,569,485 + +1.7 2,569,485 - -0.4 2,569,490 + +0.4 2,569,490 + +0.9 2,569,490 + -1.6 2,569,498 - -2.5 2,569,498 - +2.1 2,569,733 + +1.1 2,569,742 - +1.9 2,569,760 + +0.9 2,569,778 + -2.2 2,569,868 + -4.0 2,569,931 + +2.4 2,569,937 + -2.2 2,569,937 + -0.4
Or see this region's nucleotide sequence