Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11755 and BWI76_RS11760 are separated by 97 nucleotides BWI76_RS11760 and BWI76_RS11765 are separated by 74 nucleotides BWI76_RS11765 and BWI76_RS11770 are separated by 134 nucleotides BWI76_RS11770 and BWI76_RS11775 are separated by 91 nucleotides
BWI76_RS11755: BWI76_RS11755 - catalase HPII, at 2,472,558 to 2,474,816
_RS11755
BWI76_RS11760: BWI76_RS11760 - cell division protein, at 2,474,914 to 2,475,252
_RS11760
BWI76_RS11765: BWI76_RS11765 - bifunctional transporter/kinase, at 2,475,327 to 2,476,718
_RS11765
BWI76_RS11770: BWI76_RS11770 - hypothetical protein, at 2,476,853 to 2,477,443
_RS11770
BWI76_RS11775: BWI76_RS11775 - 2-deoxy-D-gluconate 3-dehydrogenase, at 2,477,535 to 2,478,296
_RS11775
Position (kb)
2475
2476
2477 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2474.794 kb on + strand at 2475.122 kb on - strand, within BWI76_RS11760 at 2475.325 kb on + strand at 2475.325 kb on + strand at 2475.455 kb on - strand at 2475.854 kb on + strand, within BWI76_RS11765 at 2476.002 kb on + strand, within BWI76_RS11765 at 2476.055 kb on - strand, within BWI76_RS11765 at 2476.239 kb on - strand, within BWI76_RS11765 at 2476.273 kb on + strand, within BWI76_RS11765 at 2476.338 kb on + strand, within BWI76_RS11765 at 2476.356 kb on - strand, within BWI76_RS11765 at 2476.395 kb on - strand, within BWI76_RS11765 at 2476.532 kb on + strand, within BWI76_RS11765 at 2476.638 kb on + strand at 2476.643 kb on - strand at 2476.937 kb on + strand, within BWI76_RS11770 at 2476.976 kb on - strand, within BWI76_RS11770 at 2477.275 kb on - strand, within BWI76_RS11770 at 2477.451 kb on + strand at 2477.575 kb on - strand at 2477.628 kb on - strand, within BWI76_RS11775
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 2,474,794 + -0.2 2,475,122 - BWI76_RS11760 0.61 -2.4 2,475,325 + -0.2 2,475,325 + -0.0 2,475,455 - +0.6 2,475,854 + BWI76_RS11765 0.38 -0.2 2,476,002 + BWI76_RS11765 0.48 -3.0 2,476,055 - BWI76_RS11765 0.52 -0.1 2,476,239 - BWI76_RS11765 0.66 -1.5 2,476,273 + BWI76_RS11765 0.68 -4.0 2,476,338 + BWI76_RS11765 0.73 -0.7 2,476,356 - BWI76_RS11765 0.74 -0.3 2,476,395 - BWI76_RS11765 0.77 -2.3 2,476,532 + BWI76_RS11765 0.87 -1.7 2,476,638 + +0.8 2,476,643 - +0.1 2,476,937 + BWI76_RS11770 0.14 +0.9 2,476,976 - BWI76_RS11770 0.21 +0.7 2,477,275 - BWI76_RS11770 0.71 -0.2 2,477,451 + +0.3 2,477,575 - -0.7 2,477,628 - BWI76_RS11775 0.12 -4.1
Or see this region's nucleotide sequence