Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07705 and BWI76_RS07710 are separated by 27 nucleotides BWI76_RS07710 and BWI76_RS07715 are separated by 10 nucleotides BWI76_RS07715 and BWI76_RS07720 are separated by 85 nucleotides BWI76_RS07720 and BWI76_RS07725 overlap by 4 nucleotides
BWI76_RS07705: BWI76_RS07705 - enterochelin esterase, at 1,632,362 to 1,633,561
_RS07705
BWI76_RS07710: BWI76_RS07710 - invasin-like protein, at 1,633,589 to 1,633,792
_RS07710
BWI76_RS07715: BWI76_RS07715 - non-ribosomal peptide synthetase, at 1,633,803 to 1,637,690
_RS07715
BWI76_RS07720: BWI76_RS07720 - iron-enterobactin transporter ATP-binding protein, at 1,637,776 to 1,638,570
_RS07720
BWI76_RS07725: BWI76_RS07725 - iron-enterobactin transporter permease, at 1,638,567 to 1,639,559
_RS07725
Position (kb) 1633
1634
1635
1636
1637
1638 Strain fitness (log2 ratio) -1
0
1
2 at 1632.875 kb on - strand, within BWI76_RS07705 at 1632.971 kb on + strand, within BWI76_RS07705 at 1633.541 kb on + strand at 1633.763 kb on + strand, within BWI76_RS07710 at 1633.763 kb on + strand, within BWI76_RS07710 at 1633.763 kb on + strand, within BWI76_RS07710 at 1633.897 kb on + strand at 1633.905 kb on - strand at 1633.972 kb on + strand at 1633.980 kb on - strand at 1634.061 kb on - strand at 1634.158 kb on + strand at 1634.397 kb on + strand, within BWI76_RS07715 at 1634.410 kb on - strand, within BWI76_RS07715 at 1634.558 kb on - strand, within BWI76_RS07715 at 1634.877 kb on + strand, within BWI76_RS07715 at 1634.999 kb on + strand, within BWI76_RS07715 at 1635.222 kb on - strand, within BWI76_RS07715 at 1635.739 kb on + strand, within BWI76_RS07715 at 1635.802 kb on - strand, within BWI76_RS07715 at 1636.142 kb on - strand, within BWI76_RS07715 at 1636.209 kb on - strand, within BWI76_RS07715 at 1636.232 kb on + strand, within BWI76_RS07715 at 1636.240 kb on - strand, within BWI76_RS07715 at 1636.259 kb on - strand, within BWI76_RS07715 at 1636.334 kb on + strand, within BWI76_RS07715 at 1637.073 kb on - strand, within BWI76_RS07715 at 1637.606 kb on + strand at 1637.754 kb on + strand at 1638.416 kb on - strand, within BWI76_RS07720 at 1638.551 kb on + strand at 1638.661 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 1,632,875 - BWI76_RS07705 0.43 -0.5 1,632,971 + BWI76_RS07705 0.51 -0.6 1,633,541 + +2.6 1,633,763 + BWI76_RS07710 0.85 +0.4 1,633,763 + BWI76_RS07710 0.85 +0.8 1,633,763 + BWI76_RS07710 0.85 -0.0 1,633,897 + +0.7 1,633,905 - +0.7 1,633,972 + +0.6 1,633,980 - -0.1 1,634,061 - -0.2 1,634,158 + +0.2 1,634,397 + BWI76_RS07715 0.15 +1.0 1,634,410 - BWI76_RS07715 0.16 +1.1 1,634,558 - BWI76_RS07715 0.19 +0.8 1,634,877 + BWI76_RS07715 0.28 +0.5 1,634,999 + BWI76_RS07715 0.31 +2.1 1,635,222 - BWI76_RS07715 0.36 +1.3 1,635,739 + BWI76_RS07715 0.50 +0.9 1,635,802 - BWI76_RS07715 0.51 +1.0 1,636,142 - BWI76_RS07715 0.60 +2.1 1,636,209 - BWI76_RS07715 0.62 +0.1 1,636,232 + BWI76_RS07715 0.62 +1.5 1,636,240 - BWI76_RS07715 0.63 +0.5 1,636,259 - BWI76_RS07715 0.63 +1.0 1,636,334 + BWI76_RS07715 0.65 +2.2 1,637,073 - BWI76_RS07715 0.84 +2.2 1,637,606 + +1.2 1,637,754 + +0.1 1,638,416 - BWI76_RS07720 0.81 +1.5 1,638,551 + +0.6 1,638,661 + +2.3
Or see this region's nucleotide sequence