Experiment: K. M5a1 KLEB10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05405 and BWI76_RS05410 are separated by 163 nucleotides BWI76_RS05410 and BWI76_RS05415 are separated by 30 nucleotides BWI76_RS05415 and BWI76_RS05420 are separated by 104 nucleotides
BWI76_RS05405: BWI76_RS05405 - tRNA-Asp, at 1,135,931 to 1,136,007
_RS05405
BWI76_RS05410: BWI76_RS05410 - 2,5-didehydrogluconate reductase B, at 1,136,171 to 1,136,974
_RS05410
BWI76_RS05415: BWI76_RS05415 - LysR family transcriptional regulator, at 1,137,005 to 1,137,907
_RS05415
BWI76_RS05420: BWI76_RS05420 - MFS transporter, at 1,138,012 to 1,139,202
_RS05420
Position (kb)
1137
1138 Strain fitness (log2 ratio)
-2
-1
0
1 at 1136.583 kb on - strand, within BWI76_RS05410 at 1136.637 kb on + strand, within BWI76_RS05410 at 1136.675 kb on + strand, within BWI76_RS05410 at 1136.785 kb on + strand, within BWI76_RS05410 at 1137.065 kb on - strand at 1137.349 kb on + strand, within BWI76_RS05415 at 1137.383 kb on - strand, within BWI76_RS05415 at 1137.448 kb on + strand, within BWI76_RS05415 at 1137.448 kb on + strand at 1137.456 kb on - strand, within BWI76_RS05415 at 1137.456 kb on - strand, within BWI76_RS05415 at 1137.471 kb on - strand, within BWI76_RS05415 at 1138.097 kb on + strand at 1138.250 kb on - strand, within BWI76_RS05420 at 1138.258 kb on - strand, within BWI76_RS05420 at 1138.364 kb on + strand, within BWI76_RS05420 at 1138.421 kb on + strand, within BWI76_RS05420 at 1138.507 kb on - strand, within BWI76_RS05420 at 1138.532 kb on + strand, within BWI76_RS05420 at 1138.540 kb on - strand, within BWI76_RS05420 at 1138.597 kb on + strand, within BWI76_RS05420 at 1138.631 kb on + strand, within BWI76_RS05420 at 1138.714 kb on - strand, within BWI76_RS05420
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 10 remove 1,136,583 - BWI76_RS05410 0.51 +0.3 1,136,637 + BWI76_RS05410 0.58 -0.8 1,136,675 + BWI76_RS05410 0.63 +0.7 1,136,785 + BWI76_RS05410 0.76 -0.5 1,137,065 - -1.4 1,137,349 + BWI76_RS05415 0.38 +0.6 1,137,383 - BWI76_RS05415 0.42 -1.3 1,137,448 + BWI76_RS05415 0.49 -1.3 1,137,448 + -2.1 1,137,456 - BWI76_RS05415 0.50 +0.5 1,137,456 - BWI76_RS05415 0.50 -1.7 1,137,471 - BWI76_RS05415 0.52 -1.7 1,138,097 + -0.3 1,138,250 - BWI76_RS05420 0.20 -0.6 1,138,258 - BWI76_RS05420 0.21 +1.3 1,138,364 + BWI76_RS05420 0.30 +0.1 1,138,421 + BWI76_RS05420 0.34 -0.8 1,138,507 - BWI76_RS05420 0.42 +0.2 1,138,532 + BWI76_RS05420 0.44 +0.7 1,138,540 - BWI76_RS05420 0.44 +0.1 1,138,597 + BWI76_RS05420 0.49 -0.5 1,138,631 + BWI76_RS05420 0.52 +0.5 1,138,714 - BWI76_RS05420 0.59 +0.4
Or see this region's nucleotide sequence