Experiment: K. M5a1 KLEB22 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26810 and BWI76_RS26815 are separated by 83 nucleotides BWI76_RS26815 and BWI76_RS26820 are separated by 351 nucleotides BWI76_RS26820 and BWI76_RS26825 overlap by 20 nucleotides BWI76_RS26825 and BWI76_RS26830 are separated by 48 nucleotides BWI76_RS26830 and BWI76_RS26835 are separated by 11 nucleotides BWI76_RS26835 and BWI76_RS26840 are separated by 2 nucleotides
BWI76_RS26810: BWI76_RS26810 - cellulose biosynthesis protein BcsG, at 5,558,766 to 5,560,442
_RS26810
BWI76_RS26815: BWI76_RS26815 - small toxic polypeptide ldrD (Modular protein), at 5,560,526 to 5,560,651
_RS26815
BWI76_RS26820: BWI76_RS26820 - small toxic polypeptide ldrD (Modular protein), at 5,561,003 to 5,561,128
_RS26820
BWI76_RS26825: BWI76_RS26825 - hypothetical protein, at 5,561,109 to 5,561,327
_RS26825
BWI76_RS26830: BWI76_RS26830 - endoglucanase, at 5,561,376 to 5,562,380
_RS26830
BWI76_RS26835: BWI76_RS26835 - hypothetical protein, at 5,562,392 to 5,562,865
_RS26835
BWI76_RS26840: BWI76_RS26840 - cellulose synthase, at 5,562,868 to 5,566,920
_RS26840
Position (kb)
5561
5562
5563 Strain fitness (log2 ratio)
-2
-1
0
1 at 5560.399 kb on + strand at 5560.492 kb on - strand at 5560.729 kb on - strand at 5560.964 kb on - strand at 5561.144 kb on + strand, within BWI76_RS26825 at 5561.342 kb on + strand at 5561.398 kb on + strand at 5561.477 kb on + strand, within BWI76_RS26830 at 5561.477 kb on + strand, within BWI76_RS26830 at 5561.544 kb on - strand, within BWI76_RS26830 at 5561.772 kb on + strand, within BWI76_RS26830 at 5561.829 kb on + strand, within BWI76_RS26830 at 5561.860 kb on + strand, within BWI76_RS26830 at 5561.868 kb on - strand, within BWI76_RS26830 at 5561.876 kb on - strand, within BWI76_RS26830 at 5562.152 kb on + strand, within BWI76_RS26830 at 5562.160 kb on - strand, within BWI76_RS26830 at 5562.324 kb on - strand at 5562.454 kb on - strand, within BWI76_RS26835 at 5562.471 kb on + strand, within BWI76_RS26835 at 5562.478 kb on - strand, within BWI76_RS26835 at 5562.484 kb on - strand, within BWI76_RS26835 at 5562.523 kb on + strand, within BWI76_RS26835 at 5562.604 kb on + strand, within BWI76_RS26835 at 5562.612 kb on - strand, within BWI76_RS26835 at 5562.612 kb on - strand, within BWI76_RS26835 at 5562.622 kb on - strand, within BWI76_RS26835 at 5562.657 kb on + strand, within BWI76_RS26835 at 5562.665 kb on - strand, within BWI76_RS26835 at 5562.969 kb on - strand at 5562.975 kb on - strand at 5563.055 kb on + strand at 5563.057 kb on + strand at 5563.119 kb on + strand at 5563.123 kb on - strand at 5563.142 kb on - strand at 5563.219 kb on + strand at 5563.355 kb on - strand, within BWI76_RS26840
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 0.1 remove 5,560,399 + -0.9 5,560,492 - +0.1 5,560,729 - -0.1 5,560,964 - +0.7 5,561,144 + BWI76_RS26825 0.16 +0.5 5,561,342 + +0.1 5,561,398 + +0.1 5,561,477 + BWI76_RS26830 0.10 -0.7 5,561,477 + BWI76_RS26830 0.10 -1.2 5,561,544 - BWI76_RS26830 0.17 -0.5 5,561,772 + BWI76_RS26830 0.39 -0.6 5,561,829 + BWI76_RS26830 0.45 +0.3 5,561,860 + BWI76_RS26830 0.48 -0.7 5,561,868 - BWI76_RS26830 0.49 +0.3 5,561,876 - BWI76_RS26830 0.50 -0.3 5,562,152 + BWI76_RS26830 0.77 -0.5 5,562,160 - BWI76_RS26830 0.78 -0.6 5,562,324 - -0.2 5,562,454 - BWI76_RS26835 0.13 +1.0 5,562,471 + BWI76_RS26835 0.17 +0.3 5,562,478 - BWI76_RS26835 0.18 +0.3 5,562,484 - BWI76_RS26835 0.19 +0.1 5,562,523 + BWI76_RS26835 0.28 -0.2 5,562,604 + BWI76_RS26835 0.45 +0.4 5,562,612 - BWI76_RS26835 0.46 -0.5 5,562,612 - BWI76_RS26835 0.46 +0.2 5,562,622 - BWI76_RS26835 0.49 +0.2 5,562,657 + BWI76_RS26835 0.56 -2.0 5,562,665 - BWI76_RS26835 0.58 -0.7 5,562,969 - +0.2 5,562,975 - -0.6 5,563,055 + -0.8 5,563,057 + +0.2 5,563,119 + +0.4 5,563,123 - +0.1 5,563,142 - -0.7 5,563,219 + +0.0 5,563,355 - BWI76_RS26840 0.12 +0.1
Or see this region's nucleotide sequence