Experiment: K. M5a1 KLEB22 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20965 and BWI76_RS20970 overlap by 4 nucleotides BWI76_RS20970 and BWI76_RS20975 are separated by 43 nucleotides BWI76_RS20975 and BWI76_RS20980 are separated by 422 nucleotides
BWI76_RS20965: BWI76_RS20965 - phosphoribosylformylglycinamidine cyclo-ligase, at 4,382,370 to 4,383,407
_RS20965
BWI76_RS20970: BWI76_RS20970 - phosphoribosylglycinamide formyltransferase, at 4,383,404 to 4,384,045
_RS20970
BWI76_RS20975: BWI76_RS20975 - hypothetical protein, at 4,384,089 to 4,385,447
_RS20975
BWI76_RS20980: BWI76_RS20980 - MFS transporter, at 4,385,870 to 4,387,324
_RS20980
Position (kb) 4384
4385
4386 Strain fitness (log2 ratio) -2
-1
0
1 at 4383.163 kb on - strand, within BWI76_RS20965 at 4383.337 kb on - strand at 4383.346 kb on - strand at 4383.379 kb on + strand at 4383.431 kb on + strand at 4383.859 kb on + strand, within BWI76_RS20970 at 4384.255 kb on + strand, within BWI76_RS20975 at 4384.435 kb on - strand, within BWI76_RS20975 at 4384.529 kb on - strand, within BWI76_RS20975 at 4384.759 kb on - strand, within BWI76_RS20975 at 4384.787 kb on - strand, within BWI76_RS20975 at 4384.884 kb on + strand, within BWI76_RS20975 at 4384.922 kb on - strand, within BWI76_RS20975 at 4384.923 kb on - strand, within BWI76_RS20975 at 4384.953 kb on - strand, within BWI76_RS20975 at 4384.978 kb on + strand, within BWI76_RS20975 at 4384.983 kb on + strand, within BWI76_RS20975 at 4385.068 kb on - strand, within BWI76_RS20975 at 4385.205 kb on + strand, within BWI76_RS20975 at 4385.213 kb on - strand, within BWI76_RS20975 at 4385.213 kb on - strand, within BWI76_RS20975 at 4385.231 kb on - strand, within BWI76_RS20975 at 4385.407 kb on + strand at 4385.408 kb on - strand at 4385.512 kb on + strand at 4385.515 kb on - strand at 4385.522 kb on + strand at 4385.651 kb on + strand at 4385.717 kb on + strand at 4385.717 kb on + strand at 4385.717 kb on + strand at 4385.764 kb on + strand at 4385.785 kb on - strand at 4385.820 kb on + strand at 4385.863 kb on - strand at 4385.966 kb on + strand at 4386.027 kb on - strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.107 kb on - strand, within BWI76_RS20980 at 4386.112 kb on - strand, within BWI76_RS20980 at 4386.117 kb on - strand, within BWI76_RS20980 at 4386.143 kb on - strand, within BWI76_RS20980 at 4386.187 kb on - strand, within BWI76_RS20980 at 4386.195 kb on + strand, within BWI76_RS20980 at 4386.195 kb on + strand, within BWI76_RS20980 at 4386.195 kb on + strand, within BWI76_RS20980 at 4386.203 kb on - strand, within BWI76_RS20980 at 4386.203 kb on - strand, within BWI76_RS20980 at 4386.203 kb on - strand, within BWI76_RS20980 at 4386.272 kb on - strand, within BWI76_RS20980 at 4386.369 kb on - strand, within BWI76_RS20980 at 4386.374 kb on + strand, within BWI76_RS20980 at 4386.393 kb on - strand, within BWI76_RS20980 at 4386.408 kb on + strand, within BWI76_RS20980
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 0.1 remove 4,383,163 - BWI76_RS20965 0.76 +0.0 4,383,337 - -0.3 4,383,346 - -0.3 4,383,379 + -0.4 4,383,431 + +0.0 4,383,859 + BWI76_RS20970 0.71 +0.3 4,384,255 + BWI76_RS20975 0.12 +0.0 4,384,435 - BWI76_RS20975 0.25 -0.8 4,384,529 - BWI76_RS20975 0.32 -0.2 4,384,759 - BWI76_RS20975 0.49 -2.0 4,384,787 - BWI76_RS20975 0.51 +0.0 4,384,884 + BWI76_RS20975 0.58 -0.9 4,384,922 - BWI76_RS20975 0.61 +0.5 4,384,923 - BWI76_RS20975 0.61 -0.3 4,384,953 - BWI76_RS20975 0.64 +0.7 4,384,978 + BWI76_RS20975 0.65 +0.6 4,384,983 + BWI76_RS20975 0.66 +0.5 4,385,068 - BWI76_RS20975 0.72 -0.8 4,385,205 + BWI76_RS20975 0.82 -0.1 4,385,213 - BWI76_RS20975 0.83 +0.2 4,385,213 - BWI76_RS20975 0.83 +0.3 4,385,231 - BWI76_RS20975 0.84 +0.4 4,385,407 + -1.4 4,385,408 - -0.0 4,385,512 + -0.3 4,385,515 - -0.8 4,385,522 + +0.9 4,385,651 + -0.3 4,385,717 + +0.3 4,385,717 + -0.1 4,385,717 + -1.4 4,385,764 + +0.1 4,385,785 - +0.5 4,385,820 + +0.0 4,385,863 - +0.1 4,385,966 + -1.0 4,386,027 - BWI76_RS20980 0.11 +0.3 4,386,104 + BWI76_RS20980 0.16 -0.0 4,386,104 + BWI76_RS20980 0.16 -0.7 4,386,104 + BWI76_RS20980 0.16 +0.6 4,386,104 + BWI76_RS20980 0.16 +0.4 4,386,104 + BWI76_RS20980 0.16 +0.3 4,386,107 - BWI76_RS20980 0.16 +0.2 4,386,112 - BWI76_RS20980 0.17 -0.2 4,386,117 - BWI76_RS20980 0.17 +0.0 4,386,143 - BWI76_RS20980 0.19 +0.3 4,386,187 - BWI76_RS20980 0.22 +0.5 4,386,195 + BWI76_RS20980 0.22 -0.1 4,386,195 + BWI76_RS20980 0.22 +0.1 4,386,195 + BWI76_RS20980 0.22 +1.0 4,386,203 - BWI76_RS20980 0.23 -0.4 4,386,203 - BWI76_RS20980 0.23 -0.7 4,386,203 - BWI76_RS20980 0.23 +0.1 4,386,272 - BWI76_RS20980 0.28 +0.0 4,386,369 - BWI76_RS20980 0.34 -0.9 4,386,374 + BWI76_RS20980 0.35 +0.8 4,386,393 - BWI76_RS20980 0.36 -0.4 4,386,408 + BWI76_RS20980 0.37 -0.1
Or see this region's nucleotide sequence