Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14005

Experiment: K. M5a1 KLEB22 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13995 and BWI76_RS14000 are separated by 103 nucleotidesBWI76_RS14000 and BWI76_RS14005 are separated by 2 nucleotidesBWI76_RS14005 and BWI76_RS14010 are separated by 116 nucleotides BWI76_RS13995: BWI76_RS13995 - penicillin-binding protein 2, at 2,923,874 to 2,925,793 _RS13995 BWI76_RS14000: BWI76_RS14000 - glutamine amidotransferase, at 2,925,897 to 2,926,640 _RS14000 BWI76_RS14005: BWI76_RS14005 - TetR family transcriptional regulator, at 2,926,643 to 2,927,191 _RS14005 BWI76_RS14010: BWI76_RS14010 - SDR family oxidoreductase, at 2,927,308 to 2,928,090 _RS14010 Position (kb) 2926 2927 2928Strain fitness (log2 ratio) -1 0 1at 2926.067 kb on + strand, within BWI76_RS14000at 2926.339 kb on - strand, within BWI76_RS14000at 2926.343 kb on + strand, within BWI76_RS14000at 2926.523 kb on - strand, within BWI76_RS14000at 2926.570 kb on + strandat 2926.816 kb on + strand, within BWI76_RS14005at 2927.693 kb on + strand, within BWI76_RS14010at 2927.773 kb on + strand, within BWI76_RS14010at 2927.810 kb on + strand, within BWI76_RS14010at 2927.957 kb on + strand, within BWI76_RS14010at 2928.097 kb on + strandat 2928.114 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 0.1
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2,926,067 + BWI76_RS14000 0.23 -0.0
2,926,339 - BWI76_RS14000 0.59 -0.2
2,926,343 + BWI76_RS14000 0.60 -0.1
2,926,523 - BWI76_RS14000 0.84 -0.2
2,926,570 + +0.2
2,926,816 + BWI76_RS14005 0.32 -0.6
2,927,693 + BWI76_RS14010 0.49 -0.4
2,927,773 + BWI76_RS14010 0.59 -0.0
2,927,810 + BWI76_RS14010 0.64 -0.1
2,927,957 + BWI76_RS14010 0.83 +1.1
2,928,097 + +1.7
2,928,114 + +0.9

Or see this region's nucleotide sequence