Experiment: K. M5a1 KLEB22 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06795 and BWI76_RS06800 are separated by 66 nucleotides BWI76_RS06800 and BWI76_RS06805 are separated by 14 nucleotides BWI76_RS06805 and BWI76_RS06810 are separated by 69 nucleotides BWI76_RS06810 and BWI76_RS06815 are separated by 150 nucleotides BWI76_RS06815 and BWI76_RS06820 are separated by 91 nucleotides
BWI76_RS06795: BWI76_RS06795 - hypothetical protein, at 1,427,507 to 1,428,067
_RS06795
BWI76_RS06800: BWI76_RS06800 - DUF2496 domain-containing protein, at 1,428,134 to 1,428,307
_RS06800
BWI76_RS06805: BWI76_RS06805 - primosomal replication protein N'', at 1,428,322 to 1,428,849
_RS06805
BWI76_RS06810: BWI76_RS06810 - hypothetical protein, at 1,428,919 to 1,429,296
_RS06810
BWI76_RS06815: BWI76_RS06815 - adenine phosphoribosyltransferase, at 1,429,447 to 1,429,998
_RS06815
BWI76_RS06820: BWI76_RS06820 - DNA polymerase III subunit gamma/tau, at 1,430,090 to 1,431,991
_RS06820
Position (kb)
1428
1429
1430 Strain fitness (log2 ratio)
-2
-1
0
1 at 1428.046 kb on + strand at 1428.274 kb on + strand, within BWI76_RS06800 at 1428.450 kb on - strand, within BWI76_RS06805 at 1428.556 kb on - strand, within BWI76_RS06805 at 1428.707 kb on + strand, within BWI76_RS06805 at 1428.879 kb on + strand at 1428.974 kb on - strand, within BWI76_RS06810 at 1429.029 kb on + strand, within BWI76_RS06810 at 1429.141 kb on - strand, within BWI76_RS06810 at 1429.250 kb on - strand, within BWI76_RS06810 at 1429.290 kb on + strand at 1429.367 kb on - strand at 1429.498 kb on + strand at 1429.498 kb on + strand at 1429.506 kb on - strand, within BWI76_RS06815 at 1429.585 kb on - strand, within BWI76_RS06815 at 1429.696 kb on - strand, within BWI76_RS06815 at 1429.891 kb on + strand, within BWI76_RS06815
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 0.1 remove 1,428,046 + +0.7 1,428,274 + BWI76_RS06800 0.80 +0.2 1,428,450 - BWI76_RS06805 0.24 -0.1 1,428,556 - BWI76_RS06805 0.44 +0.1 1,428,707 + BWI76_RS06805 0.73 -0.5 1,428,879 + -0.4 1,428,974 - BWI76_RS06810 0.15 -0.6 1,429,029 + BWI76_RS06810 0.29 -2.1 1,429,141 - BWI76_RS06810 0.59 -0.5 1,429,250 - BWI76_RS06810 0.88 -0.1 1,429,290 + +0.5 1,429,367 - -1.7 1,429,498 + +1.3 1,429,498 + +0.1 1,429,506 - BWI76_RS06815 0.11 +0.6 1,429,585 - BWI76_RS06815 0.25 -0.0 1,429,696 - BWI76_RS06815 0.45 -0.1 1,429,891 + BWI76_RS06815 0.80 +0.8
Or see this region's nucleotide sequence