Experiment: K. M5a1 KLEB22 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04435 and BWI76_RS04440 are separated by 31 nucleotides BWI76_RS04440 and BWI76_RS04445 overlap by 4 nucleotides BWI76_RS04445 and BWI76_RS04450 are separated by 10 nucleotides
BWI76_RS04435: BWI76_RS04435 - [citrate (pro-3S)-lyase] ligase, at 916,335 to 917,363
_RS04435
BWI76_RS04440: BWI76_RS04440 - citrate lyase ACP, at 917,395 to 917,688
_RS04440
BWI76_RS04445: BWI76_RS04445 - citrate (pro-3S)-lyase subunit beta, at 917,685 to 918,554
_RS04445
BWI76_RS04450: BWI76_RS04450 - citrate lyase subunit alpha, at 918,565 to 920,091
_RS04450
Position (kb)
917
918 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 916.397 kb on + strand at 916.398 kb on - strand at 916.418 kb on + strand at 916.418 kb on + strand at 916.426 kb on - strand at 916.438 kb on + strand, within BWI76_RS04435 at 916.461 kb on + strand, within BWI76_RS04435 at 916.503 kb on + strand, within BWI76_RS04435 at 916.503 kb on + strand, within BWI76_RS04435 at 916.526 kb on - strand, within BWI76_RS04435 at 916.552 kb on + strand, within BWI76_RS04435 at 916.552 kb on + strand, within BWI76_RS04435 at 916.560 kb on - strand, within BWI76_RS04435 at 916.560 kb on - strand, within BWI76_RS04435 at 916.565 kb on + strand, within BWI76_RS04435 at 916.571 kb on + strand, within BWI76_RS04435 at 916.585 kb on + strand, within BWI76_RS04435 at 916.830 kb on - strand, within BWI76_RS04435 at 917.148 kb on - strand, within BWI76_RS04435 at 917.237 kb on + strand, within BWI76_RS04435 at 917.418 kb on + strand at 917.461 kb on - strand, within BWI76_RS04440 at 917.559 kb on + strand, within BWI76_RS04440 at 917.615 kb on - strand, within BWI76_RS04440 at 917.653 kb on + strand, within BWI76_RS04440 at 917.759 kb on - strand at 917.935 kb on + strand, within BWI76_RS04445 at 918.031 kb on + strand, within BWI76_RS04445 at 918.031 kb on + strand, within BWI76_RS04445 at 918.046 kb on + strand, within BWI76_RS04445 at 918.379 kb on - strand, within BWI76_RS04445 at 918.454 kb on + strand, within BWI76_RS04445 at 918.527 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 0.1 remove 916,397 + +0.8 916,398 - -2.9 916,418 + -2.0 916,418 + -0.5 916,426 - +0.1 916,438 + BWI76_RS04435 0.10 +0.0 916,461 + BWI76_RS04435 0.12 -0.4 916,503 + BWI76_RS04435 0.16 +0.1 916,503 + BWI76_RS04435 0.16 +0.0 916,526 - BWI76_RS04435 0.19 +0.9 916,552 + BWI76_RS04435 0.21 -0.3 916,552 + BWI76_RS04435 0.21 -0.1 916,560 - BWI76_RS04435 0.22 +0.2 916,560 - BWI76_RS04435 0.22 +0.7 916,565 + BWI76_RS04435 0.22 +0.9 916,571 + BWI76_RS04435 0.23 -0.8 916,585 + BWI76_RS04435 0.24 +0.1 916,830 - BWI76_RS04435 0.48 -0.4 917,148 - BWI76_RS04435 0.79 -0.2 917,237 + BWI76_RS04435 0.88 -0.2 917,418 + -0.0 917,461 - BWI76_RS04440 0.22 -0.2 917,559 + BWI76_RS04440 0.56 -0.3 917,615 - BWI76_RS04440 0.75 -0.6 917,653 + BWI76_RS04440 0.88 -1.2 917,759 - -0.3 917,935 + BWI76_RS04445 0.29 -1.0 918,031 + BWI76_RS04445 0.40 -0.5 918,031 + BWI76_RS04445 0.40 +0.1 918,046 + BWI76_RS04445 0.41 +0.6 918,379 - BWI76_RS04445 0.80 +0.1 918,454 + BWI76_RS04445 0.88 -0.2 918,527 - -1.1
Or see this region's nucleotide sequence