Experiment: K. M5a1 KLEB22 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01095 and BWI76_RS01100 overlap by 4 nucleotides BWI76_RS01100 and BWI76_RS01105 are separated by 18 nucleotides BWI76_RS01105 and BWI76_RS01110 overlap by 23 nucleotides
BWI76_RS01095: BWI76_RS01095 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 225,560 to 226,822
_RS01095
BWI76_RS01100: BWI76_RS01100 - dTDP-glucose 4,6-dehydratase, at 226,819 to 227,887
_RS01100
BWI76_RS01105: BWI76_RS01105 - glucose-1-phosphate thymidylyltransferase, at 227,906 to 228,787
_RS01105
BWI76_RS01110: BWI76_RS01110 - TDP-fucosamine acetyltransferase, at 228,765 to 229,439
_RS01110
Position (kb) 226
227
228 Strain fitness (log2 ratio) -2
-1
0
1 at 226.034 kb on - strand, within BWI76_RS01095 at 226.533 kb on + strand, within BWI76_RS01095 at 226.541 kb on - strand, within BWI76_RS01095 at 226.711 kb on + strand at 226.776 kb on + strand at 226.901 kb on + strand at 226.918 kb on + strand at 226.979 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.140 kb on - strand, within BWI76_RS01100 at 227.142 kb on + strand, within BWI76_RS01100 at 227.227 kb on + strand, within BWI76_RS01100 at 227.235 kb on - strand, within BWI76_RS01100 at 227.642 kb on - strand, within BWI76_RS01100 at 227.921 kb on - strand at 228.196 kb on - strand, within BWI76_RS01105 at 228.249 kb on + strand, within BWI76_RS01105 at 228.263 kb on - strand, within BWI76_RS01105 at 228.273 kb on - strand, within BWI76_RS01105 at 228.384 kb on + strand, within BWI76_RS01105 at 228.654 kb on + strand, within BWI76_RS01105 at 228.789 kb on - strand at 228.839 kb on - strand, within BWI76_RS01110
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 0.1 remove 226,034 - BWI76_RS01095 0.38 +0.9 226,533 + BWI76_RS01095 0.77 -1.9 226,541 - BWI76_RS01095 0.78 +0.1 226,711 + -1.1 226,776 + -0.5 226,901 + +0.2 226,918 + +0.2 226,979 + BWI76_RS01100 0.15 -1.1 227,132 + BWI76_RS01100 0.29 +0.0 227,132 + BWI76_RS01100 0.29 -2.3 227,132 + BWI76_RS01100 0.29 +0.2 227,140 - BWI76_RS01100 0.30 -0.3 227,142 + BWI76_RS01100 0.30 -0.6 227,227 + BWI76_RS01100 0.38 -0.3 227,235 - BWI76_RS01100 0.39 -1.0 227,642 - BWI76_RS01100 0.77 -1.2 227,921 - +0.9 228,196 - BWI76_RS01105 0.33 -0.1 228,249 + BWI76_RS01105 0.39 +0.7 228,263 - BWI76_RS01105 0.40 +0.1 228,273 - BWI76_RS01105 0.42 -0.9 228,384 + BWI76_RS01105 0.54 -0.4 228,654 + BWI76_RS01105 0.85 -0.2 228,789 - -0.4 228,839 - BWI76_RS01110 0.11 +0.3
Or see this region's nucleotide sequence