Experiment: K. M5a1 KLEB22 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01075 and BWI76_RS01080 are separated by 242 nucleotides BWI76_RS01080 and BWI76_RS01085 are separated by 21 nucleotides
BWI76_RS01075: BWI76_RS01075 - transcription termination factor Rho, at 220,712 to 221,971
_RS01075
BWI76_RS01080: BWI76_RS01080 - undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase, at 222,214 to 223,308
_RS01080
BWI76_RS01085: BWI76_RS01085 - polysaccharide chain length modulation protein, at 223,330 to 224,376
_RS01085
Position (kb) 222
223
224 Strain fitness (log2 ratio) -1
0
1
2
3 at 222.063 kb on + strand at 222.063 kb on + strand at 222.071 kb on - strand at 222.071 kb on - strand at 222.073 kb on - strand at 222.081 kb on - strand at 222.081 kb on - strand at 222.137 kb on + strand at 222.142 kb on + strand at 222.145 kb on - strand at 222.157 kb on + strand at 222.159 kb on - strand at 222.171 kb on + strand at 222.171 kb on + strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.196 kb on + strand at 222.196 kb on + strand at 222.201 kb on + strand at 222.203 kb on + strand at 222.224 kb on - strand at 222.270 kb on + strand at 222.310 kb on - strand at 222.310 kb on - strand at 222.345 kb on - strand, within BWI76_RS01080 at 222.353 kb on - strand, within BWI76_RS01080 at 222.434 kb on + strand, within BWI76_RS01080 at 222.435 kb on + strand, within BWI76_RS01080 at 222.439 kb on + strand, within BWI76_RS01080 at 222.441 kb on - strand, within BWI76_RS01080 at 222.441 kb on - strand, within BWI76_RS01080 at 222.447 kb on - strand, within BWI76_RS01080 at 222.447 kb on - strand, within BWI76_RS01080 at 222.449 kb on + strand, within BWI76_RS01080 at 222.496 kb on - strand, within BWI76_RS01080 at 222.612 kb on - strand, within BWI76_RS01080 at 222.629 kb on + strand, within BWI76_RS01080 at 222.647 kb on - strand, within BWI76_RS01080 at 222.703 kb on + strand, within BWI76_RS01080 at 222.762 kb on - strand, within BWI76_RS01080 at 222.842 kb on + strand, within BWI76_RS01080 at 222.921 kb on + strand, within BWI76_RS01080 at 222.935 kb on + strand, within BWI76_RS01080 at 223.009 kb on - strand, within BWI76_RS01080 at 223.086 kb on + strand, within BWI76_RS01080 at 223.116 kb on - strand, within BWI76_RS01080 at 223.133 kb on + strand, within BWI76_RS01080 at 223.238 kb on + strand at 223.261 kb on - strand at 223.301 kb on - strand at 223.325 kb on - strand at 223.326 kb on + strand at 223.334 kb on - strand at 223.419 kb on + strand at 223.438 kb on + strand, within BWI76_RS01085 at 223.608 kb on + strand, within BWI76_RS01085 at 223.697 kb on + strand, within BWI76_RS01085 at 223.917 kb on + strand, within BWI76_RS01085 at 224.006 kb on + strand, within BWI76_RS01085 at 224.069 kb on + strand, within BWI76_RS01085 at 224.097 kb on + strand, within BWI76_RS01085 at 224.119 kb on - strand, within BWI76_RS01085 at 224.184 kb on - strand, within BWI76_RS01085
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 0.1 remove 222,063 + +0.5 222,063 + +1.2 222,071 - +0.7 222,071 - +1.0 222,073 - +1.0 222,081 - -0.5 222,081 - +0.0 222,137 + +0.1 222,142 + -0.3 222,145 - -0.5 222,157 + -0.5 222,159 - -0.3 222,171 + -0.0 222,171 + -1.6 222,179 - -1.0 222,179 - -1.4 222,179 - -0.4 222,179 - +0.4 222,179 - -1.1 222,179 - -0.7 222,196 + -0.7 222,196 + -1.0 222,201 + -0.1 222,203 + -0.1 222,224 - +1.1 222,270 + +0.6 222,310 - -0.3 222,310 - +0.1 222,345 - BWI76_RS01080 0.12 -0.5 222,353 - BWI76_RS01080 0.13 -0.4 222,434 + BWI76_RS01080 0.20 -0.1 222,435 + BWI76_RS01080 0.20 +0.1 222,439 + BWI76_RS01080 0.21 +0.2 222,441 - BWI76_RS01080 0.21 -0.9 222,441 - BWI76_RS01080 0.21 +0.9 222,447 - BWI76_RS01080 0.21 -1.2 222,447 - BWI76_RS01080 0.21 +0.1 222,449 + BWI76_RS01080 0.21 -1.1 222,496 - BWI76_RS01080 0.26 +0.6 222,612 - BWI76_RS01080 0.36 +0.9 222,629 + BWI76_RS01080 0.38 +0.5 222,647 - BWI76_RS01080 0.40 -0.9 222,703 + BWI76_RS01080 0.45 -0.6 222,762 - BWI76_RS01080 0.50 -0.2 222,842 + BWI76_RS01080 0.57 -1.0 222,921 + BWI76_RS01080 0.65 +0.3 222,935 + BWI76_RS01080 0.66 -1.0 223,009 - BWI76_RS01080 0.73 -0.7 223,086 + BWI76_RS01080 0.80 -1.2 223,116 - BWI76_RS01080 0.82 -1.7 223,133 + BWI76_RS01080 0.84 -0.4 223,238 + +0.6 223,261 - -0.5 223,301 - -0.5 223,325 - +0.2 223,326 + +0.6 223,334 - -0.0 223,419 + -1.7 223,438 + BWI76_RS01085 0.10 -0.7 223,608 + BWI76_RS01085 0.27 +2.9 223,697 + BWI76_RS01085 0.35 -0.3 223,917 + BWI76_RS01085 0.56 -0.3 224,006 + BWI76_RS01085 0.65 +0.0 224,069 + BWI76_RS01085 0.71 +0.5 224,097 + BWI76_RS01085 0.73 -0.7 224,119 - BWI76_RS01085 0.75 +0.5 224,184 - BWI76_RS01085 0.82 +0.1
Or see this region's nucleotide sequence