Experiment: K. M5a1 KLEB22 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27740 and BWI76_RS27745 are separated by 294 nucleotides BWI76_RS27745 and BWI76_RS27750 are separated by 11 nucleotides BWI76_RS27750 and BWI76_RS27755 are separated by 107 nucleotides BWI76_RS27755 and BWI76_RS27760 are separated by 102 nucleotides
BWI76_RS27740: BWI76_RS27740 - LacI family transcriptional regulator, at 5,756,457 to 5,757,398
_RS27740
BWI76_RS27745: BWI76_RS27745 - PTS sugar transporter subunit IIB, at 5,757,693 to 5,758,004
_RS27745
BWI76_RS27750: BWI76_RS27750 - PTS lactose/cellobiose family transporter subunit IIC, at 5,758,016 to 5,759,341
_RS27750
BWI76_RS27755: BWI76_RS27755 - molecular chaperone TorD, at 5,759,449 to 5,759,751
_RS27755
BWI76_RS27760: BWI76_RS27760 - lactam utilization protein LamB, at 5,759,854 to 5,761,233
_RS27760
Position (kb)
5758
5759
5760 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5757.066 kb on + strand, within BWI76_RS27740 at 5757.092 kb on - strand, within BWI76_RS27740 at 5757.105 kb on - strand, within BWI76_RS27740 at 5757.187 kb on + strand, within BWI76_RS27740 at 5757.338 kb on - strand at 5757.437 kb on - strand at 5757.598 kb on + strand at 5757.604 kb on - strand at 5757.700 kb on + strand at 5757.713 kb on - strand at 5757.776 kb on - strand, within BWI76_RS27745 at 5757.799 kb on - strand, within BWI76_RS27745 at 5757.903 kb on + strand, within BWI76_RS27745 at 5757.931 kb on + strand, within BWI76_RS27745 at 5757.938 kb on + strand, within BWI76_RS27745 at 5757.999 kb on + strand at 5758.015 kb on + strand at 5758.035 kb on - strand at 5758.067 kb on - strand at 5758.080 kb on + strand at 5758.238 kb on - strand, within BWI76_RS27750 at 5758.252 kb on + strand, within BWI76_RS27750 at 5758.310 kb on - strand, within BWI76_RS27750 at 5758.344 kb on + strand, within BWI76_RS27750 at 5758.353 kb on + strand, within BWI76_RS27750 at 5758.389 kb on + strand, within BWI76_RS27750 at 5758.434 kb on - strand, within BWI76_RS27750 at 5758.491 kb on - strand, within BWI76_RS27750 at 5758.553 kb on - strand, within BWI76_RS27750 at 5758.623 kb on - strand, within BWI76_RS27750 at 5758.630 kb on + strand, within BWI76_RS27750 at 5758.666 kb on + strand, within BWI76_RS27750 at 5758.707 kb on - strand, within BWI76_RS27750 at 5758.797 kb on - strand, within BWI76_RS27750 at 5758.854 kb on - strand, within BWI76_RS27750 at 5759.017 kb on - strand, within BWI76_RS27750 at 5759.017 kb on - strand, within BWI76_RS27750 at 5759.054 kb on - strand, within BWI76_RS27750 at 5759.069 kb on - strand, within BWI76_RS27750 at 5759.069 kb on - strand, within BWI76_RS27750 at 5759.074 kb on + strand, within BWI76_RS27750 at 5759.082 kb on - strand, within BWI76_RS27750 at 5759.089 kb on + strand at 5759.097 kb on - strand, within BWI76_RS27750 at 5759.097 kb on - strand, within BWI76_RS27750 at 5759.121 kb on + strand, within BWI76_RS27750 at 5759.129 kb on - strand, within BWI76_RS27750 at 5759.153 kb on - strand, within BWI76_RS27750 at 5759.209 kb on - strand at 5759.234 kb on + strand at 5759.234 kb on + strand at 5759.234 kb on + strand at 5759.239 kb on + strand at 5759.242 kb on - strand at 5759.242 kb on - strand at 5759.242 kb on - strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on + strand at 5759.262 kb on - strand at 5759.267 kb on + strand at 5759.270 kb on - strand at 5759.270 kb on - strand at 5759.270 kb on - strand at 5759.270 kb on - strand at 5759.270 kb on - strand at 5759.270 kb on - strand at 5759.270 kb on - strand at 5759.312 kb on + strand at 5759.318 kb on - strand at 5759.320 kb on - strand at 5759.320 kb on - strand at 5759.330 kb on - strand at 5759.342 kb on - strand at 5759.342 kb on - strand at 5759.435 kb on + strand at 5759.435 kb on + strand at 5759.443 kb on - strand at 5759.443 kb on - strand at 5759.489 kb on + strand, within BWI76_RS27755 at 5759.494 kb on + strand, within BWI76_RS27755 at 5759.503 kb on + strand, within BWI76_RS27755 at 5759.513 kb on + strand, within BWI76_RS27755 at 5759.513 kb on + strand, within BWI76_RS27755 at 5759.513 kb on + strand, within BWI76_RS27755 at 5759.513 kb on - strand, within BWI76_RS27755 at 5759.521 kb on - strand, within BWI76_RS27755 at 5759.521 kb on - strand, within BWI76_RS27755 at 5759.521 kb on - strand, within BWI76_RS27755 at 5759.526 kb on + strand, within BWI76_RS27755 at 5759.526 kb on + strand, within BWI76_RS27755 at 5759.526 kb on + strand, within BWI76_RS27755 at 5759.526 kb on + strand, within BWI76_RS27755 at 5759.526 kb on + strand, within BWI76_RS27755 at 5759.531 kb on - strand, within BWI76_RS27755 at 5759.534 kb on - strand, within BWI76_RS27755 at 5759.534 kb on - strand, within BWI76_RS27755 at 5759.534 kb on - strand, within BWI76_RS27755 at 5759.534 kb on - strand, within BWI76_RS27755 at 5759.541 kb on + strand, within BWI76_RS27755 at 5759.549 kb on - strand, within BWI76_RS27755 at 5759.561 kb on + strand, within BWI76_RS27755 at 5759.584 kb on + strand, within BWI76_RS27755 at 5759.585 kb on - strand, within BWI76_RS27755 at 5759.586 kb on - strand, within BWI76_RS27755 at 5759.592 kb on - strand, within BWI76_RS27755 at 5759.592 kb on - strand at 5759.592 kb on - strand, within BWI76_RS27755 at 5759.592 kb on - strand, within BWI76_RS27755 at 5759.592 kb on - strand, within BWI76_RS27755 at 5759.602 kb on + strand, within BWI76_RS27755 at 5759.602 kb on + strand, within BWI76_RS27755 at 5759.602 kb on + strand, within BWI76_RS27755 at 5759.602 kb on + strand, within BWI76_RS27755 at 5759.610 kb on - strand, within BWI76_RS27755 at 5759.610 kb on - strand, within BWI76_RS27755 at 5759.613 kb on + strand, within BWI76_RS27755 at 5759.621 kb on - strand, within BWI76_RS27755 at 5759.621 kb on - strand, within BWI76_RS27755 at 5759.660 kb on - strand, within BWI76_RS27755 at 5759.668 kb on - strand, within BWI76_RS27755 at 5759.685 kb on - strand, within BWI76_RS27755 at 5759.685 kb on - strand, within BWI76_RS27755 at 5759.690 kb on - strand, within BWI76_RS27755 at 5759.690 kb on - strand, within BWI76_RS27755 at 5759.704 kb on - strand, within BWI76_RS27755 at 5759.761 kb on + strand at 5759.785 kb on + strand at 5759.794 kb on + strand at 5759.794 kb on + strand at 5759.794 kb on + strand at 5759.794 kb on + strand at 5759.794 kb on + strand at 5759.802 kb on - strand at 5759.874 kb on + strand at 5759.882 kb on - strand at 5759.894 kb on + strand at 5759.894 kb on + strand at 5759.894 kb on + strand at 5759.894 kb on + strand at 5759.894 kb on + strand at 5759.896 kb on + strand at 5759.904 kb on + strand at 5759.904 kb on + strand at 5759.930 kb on + strand at 5759.938 kb on - strand at 5759.955 kb on - strand at 5759.962 kb on - strand at 5759.969 kb on - strand at 5759.970 kb on - strand at 5759.976 kb on - strand at 5759.983 kb on - strand at 5759.983 kb on - strand at 5760.270 kb on - strand, within BWI76_RS27760
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB22 MOI 1 remove 5,757,066 + BWI76_RS27740 0.65 +1.1 5,757,092 - BWI76_RS27740 0.67 -0.3 5,757,105 - BWI76_RS27740 0.69 +0.4 5,757,187 + BWI76_RS27740 0.77 -0.2 5,757,338 - -0.0 5,757,437 - -0.1 5,757,598 + -0.7 5,757,604 - +0.3 5,757,700 + -0.0 5,757,713 - +0.5 5,757,776 - BWI76_RS27745 0.27 +0.4 5,757,799 - BWI76_RS27745 0.34 +0.2 5,757,903 + BWI76_RS27745 0.67 +1.4 5,757,931 + BWI76_RS27745 0.76 -0.8 5,757,938 + BWI76_RS27745 0.79 +0.2 5,757,999 + +1.8 5,758,015 + -0.2 5,758,035 - +0.2 5,758,067 - +0.1 5,758,080 + -0.0 5,758,238 - BWI76_RS27750 0.17 +0.8 5,758,252 + BWI76_RS27750 0.18 -0.4 5,758,310 - BWI76_RS27750 0.22 +0.3 5,758,344 + BWI76_RS27750 0.25 +0.3 5,758,353 + BWI76_RS27750 0.25 +0.2 5,758,389 + BWI76_RS27750 0.28 +0.3 5,758,434 - BWI76_RS27750 0.32 -0.3 5,758,491 - BWI76_RS27750 0.36 -0.4 5,758,553 - BWI76_RS27750 0.40 +0.4 5,758,623 - BWI76_RS27750 0.46 +0.8 5,758,630 + BWI76_RS27750 0.46 +1.0 5,758,666 + BWI76_RS27750 0.49 +0.8 5,758,707 - BWI76_RS27750 0.52 +0.8 5,758,797 - BWI76_RS27750 0.59 +0.2 5,758,854 - BWI76_RS27750 0.63 +0.6 5,759,017 - BWI76_RS27750 0.75 +0.2 5,759,017 - BWI76_RS27750 0.75 -0.1 5,759,054 - BWI76_RS27750 0.78 +0.2 5,759,069 - BWI76_RS27750 0.79 +0.1 5,759,069 - BWI76_RS27750 0.79 +1.6 5,759,074 + BWI76_RS27750 0.80 -0.3 5,759,082 - BWI76_RS27750 0.80 +0.0 5,759,089 + -0.8 5,759,097 - BWI76_RS27750 0.82 +1.1 5,759,097 - BWI76_RS27750 0.82 -0.2 5,759,121 + BWI76_RS27750 0.83 +0.1 5,759,129 - BWI76_RS27750 0.84 +0.9 5,759,153 - BWI76_RS27750 0.86 -0.2 5,759,209 - +0.0 5,759,234 + -0.1 5,759,234 + +0.2 5,759,234 + +0.2 5,759,239 + -0.5 5,759,242 - +0.7 5,759,242 - +0.2 5,759,242 - -0.3 5,759,262 + -1.8 5,759,262 + +0.4 5,759,262 + +0.1 5,759,262 + +0.3 5,759,262 + +0.2 5,759,262 + -0.1 5,759,262 + +0.1 5,759,262 + -0.2 5,759,262 + +0.3 5,759,262 + +0.1 5,759,262 + -0.7 5,759,262 - +0.4 5,759,267 + -0.5 5,759,270 - +0.0 5,759,270 - +0.3 5,759,270 - +0.4 5,759,270 - -0.9 5,759,270 - +0.5 5,759,270 - -0.5 5,759,270 - -2.0 5,759,312 + +1.0 5,759,318 - -1.4 5,759,320 - -0.9 5,759,320 - -0.1 5,759,330 - +0.5 5,759,342 - +0.2 5,759,342 - -0.8 5,759,435 + -1.4 5,759,435 + -0.3 5,759,443 - -0.2 5,759,443 - -0.4 5,759,489 + BWI76_RS27755 0.13 +0.4 5,759,494 + BWI76_RS27755 0.15 -0.4 5,759,503 + BWI76_RS27755 0.18 -0.5 5,759,513 + BWI76_RS27755 0.21 -1.3 5,759,513 + BWI76_RS27755 0.21 -0.3 5,759,513 + BWI76_RS27755 0.21 +1.6 5,759,513 - BWI76_RS27755 0.21 -0.4 5,759,521 - BWI76_RS27755 0.24 +0.5 5,759,521 - BWI76_RS27755 0.24 +1.1 5,759,521 - BWI76_RS27755 0.24 +0.9 5,759,526 + BWI76_RS27755 0.25 -0.5 5,759,526 + BWI76_RS27755 0.25 -0.2 5,759,526 + BWI76_RS27755 0.25 -1.2 5,759,526 + BWI76_RS27755 0.25 +0.2 5,759,526 + BWI76_RS27755 0.25 +0.1 5,759,531 - BWI76_RS27755 0.27 -0.3 5,759,534 - BWI76_RS27755 0.28 -0.0 5,759,534 - BWI76_RS27755 0.28 +0.8 5,759,534 - BWI76_RS27755 0.28 +1.1 5,759,534 - BWI76_RS27755 0.28 -1.6 5,759,541 + BWI76_RS27755 0.30 +0.9 5,759,549 - BWI76_RS27755 0.33 +0.4 5,759,561 + BWI76_RS27755 0.37 -0.3 5,759,584 + BWI76_RS27755 0.45 -0.5 5,759,585 - BWI76_RS27755 0.45 -0.7 5,759,586 - BWI76_RS27755 0.45 -0.3 5,759,592 - BWI76_RS27755 0.47 +0.7 5,759,592 - +1.4 5,759,592 - BWI76_RS27755 0.47 +0.4 5,759,592 - BWI76_RS27755 0.47 -0.8 5,759,592 - BWI76_RS27755 0.47 -0.1 5,759,602 + BWI76_RS27755 0.50 +0.6 5,759,602 + BWI76_RS27755 0.50 -0.2 5,759,602 + BWI76_RS27755 0.50 -0.1 5,759,602 + BWI76_RS27755 0.50 +1.0 5,759,610 - BWI76_RS27755 0.53 +0.7 5,759,610 - BWI76_RS27755 0.53 +1.4 5,759,613 + BWI76_RS27755 0.54 -1.0 5,759,621 - BWI76_RS27755 0.57 +1.2 5,759,621 - BWI76_RS27755 0.57 +0.6 5,759,660 - BWI76_RS27755 0.70 +0.4 5,759,668 - BWI76_RS27755 0.72 -1.5 5,759,685 - BWI76_RS27755 0.78 -0.0 5,759,685 - BWI76_RS27755 0.78 -0.0 5,759,690 - BWI76_RS27755 0.80 -0.2 5,759,690 - BWI76_RS27755 0.80 +0.2 5,759,704 - BWI76_RS27755 0.84 +1.4 5,759,761 + -1.2 5,759,785 + +0.1 5,759,794 + +1.3 5,759,794 + +0.4 5,759,794 + -0.0 5,759,794 + +0.2 5,759,794 + +0.5 5,759,802 - +0.2 5,759,874 + -0.3 5,759,882 - +2.1 5,759,894 + +0.2 5,759,894 + -0.0 5,759,894 + -0.1 5,759,894 + -2.0 5,759,894 + +0.2 5,759,896 + +0.1 5,759,904 + -0.3 5,759,904 + -0.4 5,759,930 + -0.4 5,759,938 - +0.3 5,759,955 - -0.7 5,759,962 - -0.9 5,759,969 - -0.4 5,759,970 - -0.2 5,759,976 - +0.0 5,759,983 - -0.1 5,759,983 - +0.6 5,760,270 - BWI76_RS27760 0.30 +0.8
Or see this region's nucleotide sequence